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Chaudhary Charan Singh Haryana Agricultural University, Hisar
Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.
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ThesisItem Open Access Development, characterization and efficacy of bionanocluster of indole acetic acid and cobalt chloride on seed germination and yield in Cotton (Gossypium hirsutum L.)(CCSHAU, Hisar, 2023-09) Vikram Singh; Mandhania, ShiwaniIn the present investigation, Co2+ and indole-3-acetic acid were combined to create chitosan- Co2+-IAA BNCs. The mean hydrodynamic diameter, PDI value and zeta-potential of BNCs were recorded as 481.9 nm, 0.536 and +22.00 mV, respectively. Effect of seed priming with 100 ppm BNCs and four time foliar application of 100 ppm BNCs on seed germination, seedling growth, and boll drop was studied in H 1098i genotype of G. hirsutum L. Cotton seeds subjected to BNCs priming displayed higher percent germination and seed vigor index-I (92.14 %) compared to hydroprimed seeds due to elevated activities of glyoxylate cycle enzymes i.e. isocitrate lyase and malate synthase, which increased the conversion of fats to carbohydrates. Four times foliar application of 100 ppm BNCs increased leaf area, plant height, total chlorophyll content, photosynthetic rate, ACC synthase activity, indole-3-acetic acid content, boll number, boll weight ultimately increasing seed cotton yield by 20.37 %. However, ACC oxidase, cellulase, pectinase activities and boll drop significantly decreased compared to control. It is concluded that application of BNCs comprising of chitosan, IAA and Co2+ via seed priming and foliar mode is novel, economical, and environment friendly.ThesisItem Open Access Studies on epidemiology, variability and management of bakanae disease (Fusarium moniliforme Sheldon) of aromatic rice (Oryzae sativa L.)(CCSHAU, Hisar, 2023-08-04) Vikram Singh; Ashwani KumarBakanae of aromatic rice is one of the most destructive disease which appear in Haryana and adjoining states especially in early transplanted rice crop. The present studies were undertaken on “epidemiology, variability and management of bakanae disease during kharif 2021 to kharif 2022”. A total of 64 isolates were collected during roveing survey in kharif 2020 and 2021 from commonly aromatic rice grown area of Haryana state. Isolates were characterized on the basis of cultural and morphological characteristics on potato dextrose agar (PDA), showed white, milky white to creamy white colour on surface and cottony to cottony fluffy texture. Three isolates showed macro-conidia whereas, rest of isolates showed micro-conidia. The macro-conidia size ranged between (22.36-28.09) × (3.01-4.71) μm in length and breadth, respectively. The size of micro-conidia ranges between (4.80- 9.92) × (1.42-2.71) μm. The growth rate of isolates varied between 12.95-25 mm/48 hrs. Molecular variability among 26 selected isolates was assessed by using different molecular primers viz. β-tubulin, Tef 1 α, Vertf and rp 32 & rp 33, SSR, ISSR, URP and RAPD. Out of forty one tested primers, 18 showed amplification and 100 % polymorphism. Among SSR, URP and RAPD, ISSR primers, polymorphic information content (PIC) values ranged between 0.28-0.99, 0.35-0.39 and 0.30-0.39, respectively. Cluster analysis divides the 26 isolates into nine groups with the genetic similarity coefficient of 43.5. Among the biochemical constituents (mg/g of dry mycelium) total sugars, phenols, free amino acid and protein content varied between 3.77 (FM 53) - 12.08 (FM 63), 3.21 (FM 59) - 11.98 (FM 20), 26.31 (FM 25) - 58.83 (FM 53), 70 (FM 36) - 112 (FM 40), respectively. Amongst weather parameters during kharif 2021 maximum temperature (Tmax) and relative humidity morning (RHM) among all seasons showed positive correlation and minimum temperature and wind velocity showed negative correlation with bakanae per cent disease incidence. Coefficient of determination (R²) values ranged between 0.85 to 0.99. Among fungicides, carbendazim 50 WP was found most effective at 50 ppm under lab conditions. Among bioagents evaluated under nursery and field conditions, Trichoderma viride (WP) showed better results. Out of 46 genotypes screened under artificial inoculation field conditions, 22 were resistant, 17 were moderately resistant, 4 were susceptible and 3 were highly susceptible. Under nursery conditions, out of 61 genotypes, 35 were resistant, 23 were moderately resistant, two were susceptible and one was highly susceptible based on two year data.ThesisItem Open Access Performance of wheat under different irrigation and nitrogen sources(CCSHAU, Hisar, 2021-10) Vikram Singh; Parveen KumarThe present investigation was carried out at Vegetable Research Farm, CCS HAU, Haryana to evaluate the performance of wheat under various irrigation and nitrogen sources. The trial was conducted in split-plot design with three replication. It consisted of three irrigation sources i.e. treated sewage water (TSW), canal water (CW) and tube well water (TW); and seven nitrogen sources i.e. control (T1), 100 % RDN through chemical fertilizers (T2), 100 % RDN through vermicompost (T3), 50 % RDN through chemical fertilizers + 50 % RDN through vermicompost (T4), 100 % RDN through FYM (T5), 50 % RDN through chemical fertilizers + 50 % RDN through FYM (T6), 50 % RDN through chemical fertilizers +25 % RDN through FYM +25 % RDN through vermicompost (T7). The organic manures was applied before sowing of crop. The results revealed that the plant population at 15 DAS did not differ significantly among various irrigation and nitrogen sources during both the kharif seasons. Maximum plant height (cm), dry matter accumulation (g/plant) and growth indices viz. leaf area index, leaf area duration, crop growth rate and chlorophyll content; and yield attributing characters i.e. number of effective tillers/m2, spike length, number of grains per spike and test weight, and yields i.e. grain, straw and biological yield were recorded with treated sewage water over canal and tubewell water. However, among nitrogen sources, the significantly highest growth parameters and indices, and yields were obtained under application of 100 % RDN through chemical fertilizer (T2) was at par with 50 % RDN through fertilizers + 50 % RDN through vermicompost (T4), 50 % RDN through fertilizers + 25 % RDN through FYM + 25 % RDN through vermicompost (T7) and 50 % RDN through fertilizers + 50 % RDN through FYM (T6).The application of various irrigation and nitrogen sources gave non-significantly relative growth rate (mg/g/day) of wheat during both the years (2017-19). Different irrigation treatments were failed to produce any significant effect on harvest index during both the growing seasons, while the treatments receiving chemical fertilizer application alone or in combination with organic manures (vermicompost and FYM) gave at par harvest and attraction index over control. The significantly improved wheat grain quality in terms of protein content, protein yield, sedimentation value and hectolitre weight was observed under application of treated sewage water which was statistically at par with canal water. However, among nitrogen sources, the application of 100% RDN through chemical fertilizers improved the grain quality but it was at par with 50 % RDN through fertilizers + 50 % RDN through vermicompost (T4), 50 % RDN through fertilizers + 25 % RDN through FYM + 25 % RDN through vermicompost (T7) and 50 % RDN through fertilizers + 50 % RDN through FYM (T6).The treatments receiving treated sewage water gave higher nutrients (N, P, and K) concentration and their uptake in grain and straws of wheat crop during both the years. While among the nitrogen sources, treatment T2 exhibited higher nutrient accumulation over rest of the treatments. However, T2 remained at par with T4, T7 and T6. The effects of irrigation sources were non-significant on water consumptive use during both the years. It was observed that plants irrigated with treated sewage water used the water more efficiently compared to canal and tube well water. However, the effect of irrigation sources was non-significant during year 2017-18. Among nitrogen sources, the application of organic manures alone or in combination with chemical fertilizers gave comparable water use efficiency over control due to increased grain yield. During both years of the experimentation, the highest economic return regards net return and B: C ratio were attained with application of treated sewage water. Among fertilizer treatments, the highest B: C was recorded under treatments where sole application of chemical fertilizers were applied. However, integrated application of chemical fertilizers and organic manures gave higher return over sole application of manures and control. Compared to the initial soil pH values, it was reduced under use of all type of irrigation water. However, the application of treated sewage water significantly reduced soil pH as compared to canal and tubewell water. As the EC of tubewell water is high, so after two years of wheat cultivation, the soil EC was significantly increased from 0.84 to 0.91 dS/m. The build-up of soil fertility status regards was organic carbon, available N, P and K was noticed under treated sewage water irrigated plots. However, among different nitrogen sources, the significant improvement in organic carbon, available N, P and K in the soil was brought be the addition of organic manures i.e. 50 % RDN through vermicompost and farm yard manures over sole application of RDN through mineral fertilizers and control treatments. Hence, use of sewage water under wheat cultivation with integrated nitrogen application of chemical fertilizers and organic manures proved economically beneficial.