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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2010 - 20142
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Author: Suraj Kala × Start date: 2010 × Type: Thesis ×
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    ThesisItemOpen Access
    Effect of salt stress on the morphophysiological, yield and quality parameters in isabgol (Plantago ovata Forsk.) genotypes
    (CCSHAU, 2014) Suraj Kala; Varshney, U.K.
    Effect of NaCl salt stress on the morpho-physiological, yield and quality parameters in four isabgol (Plantago ovata Forsk.) genotypes viz. GI-2, HI-96, PB-80 and HI-5 was studied under screen house conditions. All the genotypes were grown in the dune sand filled polythene bags with control (without salt), 5 and 10 dSm-1 EC level of NaCl salt solution along with nutrients. Sampling was done at vegetative (58 DAS), flowering (115 DAS) and maturity (150 DAS) stage of growth. Results revealed a significant reduction in various plant growth parameters at every growth stages with increasing salt stress. Root/shoot ratio remained indifferent with the increasing salt stress level up to flowering stage but an increase in the ratio was observed at the maturity stage. Both reproductive effort as well as reproductive capacity of all the isabgol genotypes decreased with increasing salt stress. Reduction in plant growth, in general was maximum in the genotype PB-80 and minimum in GI-2. Increasing salt stress caused reduction in various yield attributes in all the isabgol genotypes. Mucilage (%) as well as seed swelling factor of all the isabgol genotypes also significantly declined under salt stress. Decline in yield as well as quality parameters was highest in the genotype PB-80 and lowest in GI-2. On the other hand increasing EC level caused enhancement of RMI, lipid peroxidation and ROS content in all the isabgol genotypes. The increase was maximum in the genotype PB-80 and minimum in GI-2. Chlotrophyll, carotenoid, ascorbic acid and total soluble protein content in leaves of all the isabgol genotypes decreased with increasing salt stress level and maximum decrease was found in the genotype PB-80 and minimum in GI-2. An accumulation of proline and total soluble carbohydrate content in leaves of all the isabgol genotypes was detected with the increase of salt stress; increase being maximum in the genotype GI-2 and minimum in PB-80. The enhancement in the activity of antioxidant enzymes viz., CAT, SOD and POD with the increase of salt stress was also highest in GI-2 and lowest in PB-80 among all the genotypes. In the Protein profile of leaves of the genotype GI-2 three new protein bands of the MW 29.59, 54.95 and 55.72 kDa and in the genotype HI-96 two new protein bands of the MW 29.59 and 55.72 kDa were detected under salt stress. While in the genotype PB-80 one protein band of MW 30.06 kDa was disappeared and one new protein band of MW 43.65 kDa was appeared under salt stress. Genotype HI-5 showed three new protein bands of MW 43.65, 55.72 and 66.07 kDa and disappearance of one protein band of MW 32.36 kDa under salt stress. The de novo synthesis of new protein bands under salt stress could be responsible for induction of salt tolerance in isabgol genotypes. While in the genotype PB-80 and HI-5 disappearance of protein bands may be viewed as marker of their salt sensitivity. Sodium and chloride content in different plant parts of isabgol genotypes increased while potassium content decreased with increasing EC level. Maximum sodium and chloride content was found in the genotype PB-80 and minimum in GI-2. Potassium content on the other hand was highest in the genotype GI-2 and lowest in PB-80. Based on the overall performance of all the genotypes under present study with respect to various morpho-physiological, yield and quality parameters, the genotype GI-2 was found to be most tolerant and the genotype PB-80 least tolerant to salt stress. Relative order of salt tolerance of different isabgol genotypes was GI-2>HI-96>HI-5>PB-80.
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    ThesisItemOpen Access
    Studies on salinity tolerance in clusterbean [Cyamopsis tetragonoloba (L.) Taub.] genotypes
    (CCSHAU, 2010) Suraj Kala; Goyal, S.C.
    The present investigation was aimed to find out the salinity tolerant genotype of clusterbean [Cyamopsis tetragonoloba (L.) Taub.] using various physiological parameters and to detect the polypeptide pattern of radicle and plumule of tolerant genotype. Thirteen genotypes of guar (HG-75, HFG-119, HG-182, HG-258, FS-277, HG-365. HG-563, HG-867, HG-870, HG-884, HG-2-20, PNB, HG-2-30) were grown in Petri plates containing five salinity levels viz. 0 (control), 4, 8, 12 and 16 dSm-1. Results revealed that progressive increase of salinity levels not only decreased the imbibition rate at 2 and 12 h of imbibition but also declined the per cent seed germination as well as the speed of germination (maguire index). Effective reduction in seed germination was detected in genotype HG-258 at higher (16 dSm-1) level of salinity. However, genotype HG-2-20 showed 100 per cent germination at all the levels of salinity. A progressive increase in salinity levels caused the decrease in plumule length, vigour index, dry weight and relative water content of radicle as well as plumule while on the other hand radicle length and dry weight of cotyledonary leaf was enhenced. The maximum enhancement in radicle length and cotyledonary leaf dry weight was observed in genotype HG-258 and HG-2-20 respectively at higher level of salinity over control. An accumulation of metabolites such as proline and total soluble sugar (TSS) in the radicle and plumule of all the guar genotypes observed with increasing salinity levels. Accumulation of proline content in radicle and plumule was maximum in genotype HG-563 and HG-2-30 respectively at higher level (16 dSm-1) of salinity. Highest accumulation of TSS in radicle and plumule was found in genotype FS-277 and PNB respectively at higher level (16 dSm-1) of salinity over control. Sodium content of radicle as well as plumule of all the genotypes of guar increased with increasing salinity level; increase being found to highest in radicle of genotype HG-365 and plumule of genotype HG-75. Potassium content of radicle as well as plumule showed decreasing trend with increasing salinity levels. K+/Na+ ratio of radicle and plumule also decreased with increasing salinity level; decrease being highest in radicle of genotype HG-870 and plumule of genotype HFG-119. On the basis of the observations taken in the score-card i.e. germination and early seedling growth, it was found that genotype HG-884 was tolerant to salinity. SDS-PAGE of radicle and plumule of genotype HG-884 showed new band of 29.86, 72.46, 79.45 and 60.96, 79.45, 92.28 kDa respectively at all the levels of salinity.