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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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  • ThesisItemOpen Access
    Persistence, mitigation and leaching of atrazine and its metabolites in soil
    (CCSHAU, Hisar, 2023-01) Priyanka; Duhan, Anil
    Persistence, mitigation and leaching potential of atrazine and its metabolites in sandy and clay loam soils was studied. Soil samples were collected periodically for dissipation and mitigation studies. Leaching experiment was performed at 50 μg (single dose) and 100 μg (double dose) in both soils taken in plexi glass columns. Enzymatic activities were studied as per the explained method in available literature. Sample preparation techniques i.e. Matrix solid phase dispersion (MSPD), was optimized using different solvents for both soils at fortification level of 0.001 μg/g for sample preparation. The limits of detection and quantification were ranged 0.0005-0.001 and 0.001-0.005μg/g, respectively for AT and metabolites. In clay loam and sandy loam soil, average recoveries of atrazine and metabolites ranged from 81.3-83.1% and 84.2-86.3%, respectively in methanol. Dissipation followed first-order kinetics. The residues reached below detectable limit after 30 and 60 days in sandy loam and clay loam soils, respectively. Presence of organic manures stimulated while BGC inhibited the dissipation of atrazine in both soils. Urease activity decreased up to 30 days and boosted thereafter up to 60 days in all treatments of clay loam soil. AT exhibited deleterious effect on urease activity up to 30 DAT followed by DEA and DIA in clay loam soil. AT and DEA stimulated while DIA inhibited DHG activity on 0 DAT in comparison to control. The residues of atrazine and its metabolites found in leachate fractions suggested about its highly mobile nature, and possible threat of surface and ground water contamination. The presence of a notable collection of residues in leachate fractions suggests the potential for surface and groundwater contamination. DEA and DIA metabolites, in particular, caused springtail Folsomia candida and earthworm Eisenia fetida to have longer and more unacceptable risks. If residues similar to those found in leachate fractions reach water bodies, they may be toxic to a variety of freshwater fish, aquatic arthropods, amphibians, and aquatic invertebrates. Future research should take a more comprehensive approach to assessing ecological health and potential threats to non-target species.
  • ThesisItemOpen Access
    Social Isolation and Cognitive Functioning in Later Life
    (Chaudhary Charan Singh Haryana Agricultural University hisar, 2022-07) Priyanka; Sangwan, Sheela
    Social isolation is a state of complete or near-complete lack of contact between an individual and society. It differs from loneliness, which reflects temporary and involuntary lack of contact with other humans in the world. Social isolation can be an issue for individuals of any age, though symptoms may differ by age group. Cognitive functioning refers to a person's ability to process thoughts. It is defined as "the ability of an individual to perform the various mental activities most closely associated with learning and problem-solving. Researchers like Cacioppo and Hawkley (2009) have highlighted the strong association between social isolation and cognitive functioning. This paper investigates the social isolation and cognitive functioning in later life. The effects of depression, anxiety and stress were explored. An intervention programme to improve the cognitive functioning of low performing elderly was imparted. The study was conducted in the rural and urban areas of Hisar, Rohtak and Karnal district of Haryana state. The results revealed that age, education and occupation, marital status and family type significantly influences the respondent’s performance. The socio-personal variables and supporting variables are the strong predictors which influence the social isolation and cognitive functioning of elderly. Depression was found to be significantly correlated with both social isolation and cognitive functioning of elderly. The findings suggest that the imparting the intervention programme may enhance the cognitive functioning in elderly.
  • ThesisItemOpen Access
    Assessment of nutritional status of anaemic pregnant women of Hisar and Fatehabad districts of Haryana
    (CCSHAU, 2017) Priyanka; Boora, Pinky
    Eight hundred fifty pregnant women were selected from the 6 villages (Bhojraj, Dahima, Kaimiri, Mirkan, Gunjar, Ladwa) of Hisar-I block and 6 villages (Chodhrywas, Kalwas, Balasmand, Bheria, Aryanagar, Dobhi) of Hisar-II block of Hisar district and 6 villages (Badopal, Chinder, Bhodia Khera, Kumharia, Dhanger, Kharakheri) of Fatehabad block and 6 villages (Kirdhan, Pilimandori, Bhattu Kalan, Bhattu Mandi, Thulan, Dhand) of Bhattu block of Fatehabad district of Haryana. Out of 850 pregnant women, screened for Hb level, 602 (70.82%) were found to be anaemic (<11g/100ml) 46.70 per cent from Hisar district and 53.29 per cent from Fatehabad district. Thus overall 70 per cent of the pregnant women were anaemic in four blocks of Hisar and Fatehabad districts. Results indicated significantly high prevalence of anaemia among pregnant women in rural areas of Haryana. Nutritional status of anaemic pregnant women was assessed using dietary survey (24h recall method), anthropometric measurements and clinical assessment which revealed that the food and nutrient intake was lower than the SDI and RDA among rural areas. Intake of cereals, pulses, fats and edible oils, green leafy vegetables, roots and tubers, other vegetables and fruits by respondents belonging to nuclear families was higher than those of respondents of joint families. The intake of cereals, milk and milk products was found to be increased with increased income of the family. Education of respondent influences the intake of food groups. Intake of energy and protein was higher by respondents of nuclear families. Intake of energy, fat, calcium, iron, β-carotene, thiamine, niacin, folic acid and vitamin B12 was found to be higher in the respondents whose average family income was Rs 18,000-27.000/month as the intake of pulses and milk products was higher by the respondents of nuclear families as compared to the respondents of joint families. The intake of food groups was higher among the respondents whose husband was engaged in service. Mean daily food and nutrient intake by respondents of Hisar district were found significantly (p≤0.01) lower as compared to Fatehabad district. Out of 300 hundred respondents, 200 respondents, 100 from Hisar and 100 from Fatehabad district were selected for imparting nutrition education and to assess food and nutrient intake. Nutrition education was imparted for a period of three months and gain in nutritional knowledge was assesed. The foods and nutrients intake of the respondents was increased significantly (p≤0.01) after imparting nutrition education. There is an urgent need to impart nutrition education to the pregnant mothers so that they can take balanced diet and improve health and nutritional status of future generation of country.
  • ThesisItemOpen Access
    Identification of molecular markers for Karnal bunt resistance in wheat (Triticum aestivum L. em. Thell)
    (CCSHAU, 2014) Priyanka; Redhu, A.S.
    Present study was carried out to screen 104 recombinant inbred lines (RILs) of cross H 567.71 (resistant) x WH 542 (susceptible) for Karnal bunt resistance and to identify SRAP and RGAP markers associated with Karnal bunt resistance in wheat. There was significant variation among all the recombinant inbred lines for percentage and coefficient of infection to Karnal bunt. Most of the RILs (68) and (71) showed up to 5% infection during the year 2011-12 and 2012-13 and thus were resistant. The percentage of infected grains or percent infection (PI) in the susceptible RILs was up to 37.14 % in the year 2011-12 and up to 31.57 % in the year 2012-13 respectively, whereas, range of coefficient of infection (CI) in the RILs was 0 to 14.21 % in the year 2011-12 and 0 to 13.81 % in the year 2012-13, respectively. Significant variations were also observed for different morphological traits such as, plant height (cm), days to flowering, days to maturity, number of tillers/meter length, spike length (cm), no. of grains/spike, 1000 grain weight, grain yield/meter length (g) and biological yield (g). A total of 80 SRAP and 121 RGAP primer combinations were used to screen parental genotypes. In SRAP, a total of 287 unambiguous bands were amplified by the 20 SRAP of 80 SRAP primer combinations, of which 123 bands were polymorphic (42.85 %). The cluster analysis led to distribution of parents and their RILs in to two major groups at the similarity coefficient value of 0.55. Major cluster I, included resistant parent H 567.71 with resistant lines and major cluster II had susceptible parent WH 542 and susceptible lines, these two groups further subdivided at similarity coefficient of 0.67 into four different clusters. In RGAP, a total of 89 unambiguous bands were amplified by the 9 of 121 RGAP primer combinations, of which 46 bands were polymorphic (51.68 %). Genetic similarity by RGAP analysis showed that parental genotypes were quite distinct from each other. The cluster analysis led to distribution of parents and their RILs in to two groups at the similarity coefficient value of 0.42. Major cluster I, included resistant parent H 567.71 with resistant lines and major cluster II had susceptible parent WH 542 and susceptible lines. These two groups further subdivided into six different clusters at similarity coefficient of 0.56. By single marker analysis, RGAP 7, RGAP 8 and RGAP 9 were directly linked with resistance genes which provide resistance against Karnal bunt. Hence, selection of Karnal bunt resistant genotypes on the basis of these three markers in early generations can be done through marker assisted selection and could be useful in future development of Karnal bunt resistant varieties.
  • ThesisItemOpen Access
    Isolation and evaluation of nodule endophytes for growth promotion in chickpea (Cicer arietinum L.)
    (CCSHAU, 2016) Priyanka; Leela Wati
    Legumes are among the World's most important crops. The family Fabaceae comprises of around 20,000 plant species in 650 different genera. Chickpea belongs to the Papilionoideae family, order Leguminosae and botanically recognized as Cicer arietinum L. As a leguminous plant, chickpea could be nodulated by rhizobial and nonrhizobial endophytic bacteria that exert several beneficial effects on host plant such as stimulation of plant growth, nitrogen fixation and induction of resistance to plant pathogen. The use of endophytes offers an attractive way to replace chemical fertilizers, pesticides and supplements. In the present investigation, a total of 107 bacterial endophytes including mesorhizobial and nonrhizobial, were isolated from healthy pink nodules of chickpea plants, collected from different districts of Haryana. Out of 107 endophytic isolates, 75% showed indole acetic acid production activity, which ranged between 0.33-19.56 µg/ml. P-solubilization activity of endophytic isolates on solid as well as liquid medium was restricted to 52% of the isolates only and their Psolubilization index (P-SI) varied from 1.0 to 3.4. Siderophore production was observed in 14% of the endophytic isolates. Twenty one of mesorhizobial endophytes out of 24, showed positive nodulation of chickpea variety HC5 under sterilized conditions. Six endophytic isolates including five nonrhizobial BE13, RE6, HE5, HE7 and ME3 and one mesorhizobial HM2, showing all the three plant growth promoting traits were further assessed for more plant growth promoting traits viz. A CC utilization, salt tolerance and potassium solubilisation. All the six endophytes showed ACC utilization activity and salt tolerance upto 4 % concentration while only endophyte, RE-6 showed potassium solubilisation activity.Three promising endophytes including two nonrhizobial RE6 and BE13 and one mesorhizobial HM2 were selected for evaluation of nodulation efficiency and plant growth parameters of chickpea under pot house conditions. Nodulation efficiency of endophyte RE-6 when coinoculated with rhizobial endophytes HM2 and phosphate solubiliser P-36 was better on the basis of nodule number, fresh wt. and dry wt. at 75 days of sowing. More seed weight, shoot dry weight and N and P content were also observed in coinoculation treatment of RE6 with HM2 and P36 while the isolate BE-13 when coinoculated with standard Rhizobium MSD-26 and phosphate solubiliser P-36 showed higher root dry weight at 75 days as well as at harvesting. Sequence analysis of 16S rDNA of endophyte BE13 showed 98% similarity with Bacillus boroniphillus and isolate RE-6 showed 99% similarity with Pseudomonas fluorescence.