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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2011 - 20142
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Author: Kharb, Anju × End date: 2014 × Start date: 2011 ×
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    ThesisItemOpen Access
    Linkage mapping of quantitative trait loci for root morphology and selection of desirable genotypes from aerobic x lowland indica crosses in rice (Oryza sativa L.)
    (CCSHAU, 2014) Kharb, Anju; Jain, Rajinder Kumar
    Identification of stable QTL for traits promoting aerobic adaptation using molecular markers can greatly enhance the efficacy of breeding programs to develop water-efficient, high yielding, aerobic rice varieties. Filial (F2 and F3) and backcross ( BC2F2 and BC1F2) populations derived from the crosses between high-yielding low-land (HKR47) and aerobic (MAS26 and MASARB25) indica rice varieties, displayed large variation for various physio-morphological traits including grain yield per plant and root traits (in case of net house evaluation). Phenotypic correlation analysis showed positive correlation (r = 0.279 and 0.351) between grain yield per plant and root length in both F2 and F3 population and between grain yield per plant and fresh & dry root weight (r = 0.232 and 0.269) in F2 population. Under aerobic field conditions, Grain yield per plant showed significant positive correlation with plant height, effective number of tillers/plant and panicle length in all the four populations. A total of 803 SSR markers, distributed on 12 rice chromosomes, were analyzed for polymorphism in two parental rice genotypes (HKR 47 and MAS 26); of these 125 (about 16%) displayed polymorphism. SSR fingerprint databases of three HKR47 x MAS26 F2 and F3 populations (94 F2 in net house, 42 F3 in net house and 52 F3 plants in field) were prepared using 125, 88 and 88 polymorphic SSR markers, respectively. NTSYS-pc UPGMA tree cluster analysis and two-dimensional PCA scaling showed scattering of the F2 and F3 populations between the two distinct parental genotypes; the populations were inclined towards MAS26. Composite interval mapping (CIM) analysis by Win QTL cartographer 2.5 revealed a total of six QTLs (qPN8.1, qPN8.2, qPN8.3, qTGW8.1, qYPP8.1 and qRL8.1) in HKR47 x MAS26 F2 population, grown in the net house on chromosome 8 (within a region of 24.9 cM) which individually explained 13.7-27.3% of the phenotypic variation. In case of HKR47 x MAS26 F3 population, based on field data and net house data, two (qTGW6.1 and qLB8.1) and six QTL (qPH3.1, qLB8.1, qTGW6.1, qTGW11.1, qRT8.1 and qRT8.2) were mapped, respectively. As many as 20 F3, 6 BC2F2 and 5 BC1F2 promising plants having higher grain yield and/or better root length/biomass were selected. Data on monitoring of putative QTL identified showed that out of twenty selected plants, four plants had 13 QTL, three plants had 12 QTL and two plants had 11 QTL in homozygous or heterozygous condition.
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    ThesisItemOpen Access
    Variation in root morphology and molecular profile in aerobic × Basmati F2 population(s)
    (CCSHAU, 2011) Kharb, Anju; Jain, R.K.
    The present investigation has been undertaken to: (i) analyze the allelic profile of 60 F2 plants obtained from a cross between Pusa1121 (Basmati) and MAS25 (aerobic) rice varieties for five SSR markers and BAD2 aroma gene, and (ii) carry out greenhouse evaluation F2 plants for root length & biomass, shoot length & biomass, yield per plant, 1000 grain weight, length-breadth ratio, aroma, relative water content and photosynthetic efficiency. Polymorphism for five microsatellite markers (RM144, RM234, RM302, RM339 and RM440) and BAD2 aroma gene specific (Bradbury et al. 2005) markers with a base difference of >30 bp in the amplified products, could be clearly visualized on 2.5% w/v agarose gels. Of the 60 F2 plants, 20, 18 and 22 plants, respectively had Pusa1121, MAS25 and Pusa1121 + MAS25 specific alleles at BAD2 locus. The NTSYS-PC UPGMA tree cluster as well as PCA analysis using six marker dataset grouped the 60 F2 plants and parental rice genotypes into two major groups: major group I with Pusa1121 and 18 F2 plants and major group II with MAS25 and 42 F2 plants. Cluster as well as PCA analysis clearly indicated that the F2 population was scattered between the two parental rice genotypes, Pusa1121 and MAS25, with an inclination towards MAS25. F2 population also showed wide variation for various traits including root length and/or dry biomass, yield per plant and length-breadth ratio. Yield per plant showed a significant positive correlation with fresh root weight (0.370; p=0.01) and dry root weight (0.328; p=0.01). A total of 9 – 12 plants had root length and/or biomass greater than MAS25 and nine plants had yield per plant higher than Pusa1121. A number of F2 plants were identified with higher root length and/or dry biomass, yield per plant, lengthbreadth ratio and with Pusa1121 specific alleles in homozygous or heterozygous condition at BAD2 locus; these lines shall serve as the novel material for the selection of stable aerobic Basmati rice varieties.