Thumbnail Image

Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

Browse

2006 - 20092
Reset filters
Subject: Zoology × Author: Sharma, Parvati × End date: 2009 × Start date: 2000 ×
Reset filters

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    ThesisItemOpen Access
    Role Of Probiotics In Controlling Epizootic Ulcerative Syndrome Disease In Carps
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2009) Sharma, Parvati; Sihag, R.C.
  • Thumbnail Image
    ThesisItemOpen Access
    Sequencing of Mx gene in an indian major carp
    (CCSHAU, 2006) Sharma, Parvati; Sihag, R.C.
    Mx gene is inducible by type I interferon and is involved in antiviral defenses. Interferons are pleiotropic glycoproteins belonging to a large family of Cytokines. The type I interferon constitute the first line of defence against viral infection in the vertebrate innate immune system. Mx gene inducible by type I interferon, natural and synthetic double stranded RNA (ds RNA) and unmethylated bacterial CpG motif, both in fish and mammalian hosts. Mx expression was observed in fish after viral infection (i.e. IPNV, VHSV, ISAV), although the antiviral role of Mx proteins in fish remains unclear. Mx gene has been cloned and sequenced in many fishes including gold fish and grass carp of family Cyprinidae. The aim of the present study was to standardize the RT-PCR protocol for the expression of Mx gene in an Indian major carp, rohu (Labeo rohita). Mx response was measured in rohu injected with the synthetic double stranded polyribonucleotide Poly I: C (500 μg/fish) and the control fish was injected with PBS alone. Total RNA was extracted from liver of acclimatized fish after 3 days of post-injection. It was transcribed to cDNA and amplification of cDNA was done by RT-PCR. The sequence was obtained from purified gel product. The blasts search was done for confirmation of Mx gene in rohu. It was found 99% matching with grass carp Mx and 98% matching with gold fish Mx3 belonging to family Cyprinidae. These results confirm the presence of Mx gene expression in an Indian major carp, rohu. The obtained partial sequence can be used for taxonomic and phylogenetic purposes.