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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2007 - 200912010 - 20111
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Subject: Zoology × Author: Geroh, Monika × End date: 2018 × Type: Thesis ×
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    ThesisItemOpen Access
    Molecular characterization of Beauveria bassiana (Balsamo) vuillemin and its bioefficacy against Tetranychus urticae Koch (Acari: Tetranychidae)
    (CCSHAU, 2011) Geroh, Monika; Gulati, Rachna
    Investigations were carried out to assess the pathogenicity of Beauveria bassiana against Tetranychus urticae on okra. Molecular characterization of six strains of B. bassiana was done by RAPD- PCR to know the genetic similarity among them. The yield of genomic DNA of B. bassiana was higher in Lysis Buffer method (2.10 to 10.50μg/ ml) as compared to CTAB method (2.34 to 7.10μg/ ml). Absorbance ratio of all strains varied from 1.60 to 2.20. Eleven random primers generated 19 (82.16%) polymorphic and 4 (17.39%) monomorphic bands. The size of PCR amplified products ranged from 500 to 4000 bp. The genetic similarity of the strains varied from 50 to 70 percent. Under in vitro 42 to 94 and 34.1 to 66.25 percent mortality of T. urticae was recorded in Direct Spray and Treated Food Bioassay, respectively with 1 × 105 to 1 × 1012 conidia ml-1 conecntrations of B. bassiana. LC50 values of B. bassiana were in the range of 3 × 105 to 2.6 × 1011 conidia ml-1 and LT50 values varied from 52.12 to 141.53 h for eggs, larvae, nymphs and adults in both the bioassay methods. Direct Spray Bioassay was more effective as compared to Treated Food Bioassay. Under field conditions, the percent reduction in T. urticae was from 51.03 to 65.18 percent with all the strains. Hb- Hyderabad (65.18%), was the most potent in reducing the mite population followed by ITCC- 4668 (62.36%), ITCC- 5408 (58.55%), ITCC- 6063 (54.61%), ITCC- 5549 (52.48%) and ITCC- 4513 (51.03%). There was no effect of B. bassiana spray S. punctillum population as their numbers in B. bassiana (strain Hb- Hyderabad) treatments (1.43 beetles/ leaf) and control (1.17 beetles/ leaf) were statistically comparable. Higher yield of okra fruits was recorded in B. bassiana treated plots (581.43 g/ plot) as compared to untreated plots (577.66 g/ plot). The Chlorophyll content was also higher in B. bassiana treated leaves (0.289 to 0.492 μg/ ml) as compared to untreated plots (0.166 μg/ ml).
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    ThesisItemOpen Access
    Ecology and management of tetranychus urticae koch on okra, abelmoschus esculentus L.
    (CCSHAU, 2007) Geroh, Monika; Gulati, Rachna
    Studies on seasonal incidence of T. urticae revealed two peaks in its population on okra during first fortnight of June (25.69 mites/ sq. cm leaf) and during first fortnight of August (22.50 mites/ sq. cm leaf). Predatory beetle S. punctillum also showed two peaks; in the first fortnight of July (0.56 beetle/leaf) and second peak coincided with peak in T. urticae population, in the first fortnight of August (0.48 beetle/leaf). Strata wise, middle strata harboured significantly more number of mites and beetles followed by top and bottom strata. A significant positive correlation(r =0.88) was observed between mite and beetle population during the present study. With abiotic factors, T. urticae and S. punctillum population showed significant positive correlation with maximum and minimum temperature. Under in vitro conditions, direct spray bioassay showed smaller LC50 and LT50 values as compared to treated food bioassay. Under in vivo, P. pinnata oil (6.24%) was most effective which caused 82.3 percent reduction in T. urticae population followed by E. globulus oil @ 4.60% (76.1%), P. pinnata seed extract @ 9.68% (60.5%) and E. globulus leaf extract @ 3.92% (36.68%). All extracts/ oil were found safe to mite predator, S. punctillum.