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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Zoology × Author: Arvind × Start date: 2004 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Molecular characterization and management of Tyrophagus putrescentiae (Schrank) in button mushroom, Agaricus bisporus (Lange)
    (CCSHAU, 2018) Arvind; Gulati, Rachna
    Molecular characterization of acaricide susceptible, resistant and untreated Tyrophagus putrescentiae on mushroom clusters having a similarity coefficient of 0.45. Cluster one was represented by untreated (control) and resistant population sharing a similarity coefficient of 0.68 where as the cluster 2 was represented by susceptible population alone. Preliminary primers screening produced 22 polymorphic bands (70.96% polymorphism) and 9 monomorphic bands (29.03%). Methanolic seed and leaf extracts of P. pinnata showed acaricidal activity against T. putrescentiae. In Direct spray and Treated arena bioassay method, maximum mortality of 82, 76 % and 75.33, 63.33% was observed at 1.0 % concentration of seed and leaf extract, respectively. Both the extracts were equally effective against T. putrescentiae when direct spray on mites was done. The mortality was 59.63 and 58.15 percent in seed and leaf extract spray, respectively. Under Treated arena bioassay, seed extract was statistically more effective against T. putrescentiae (52.22 % mortality) as compared to leaf extract (43.56 % mortality). The LC50 value in Direct spray Treated arena bioassay method was 0.77, 0.63 and 0.85 and 0.64% in leaf and seed extract of P. pinnata. Among the solvents, hexane was most potent as 8, 5.66 and 0.33 mites were recovered from 0.5, 1 and 1.5 percent concentration after 36 h as compared to 38.66 mites in control. Bioefficacy studies conducted in compost bags under direct spray showed that among the treatments, Propargite (0.05%) significantly was more effective in reducing T. putrescentiae number (0.78 mites) than P. pinnata leaf extract at 0.752% (30 mites), 0.852% (18.89 mites) and 0.952% (12.22 mites) concentrations. P. pinnata seed extract at 0. 738% reduced the population to 5.88 mites which was at par with Propargite treatment.The role of T. putrescentiae as vector of dry bubble and green mould disease caused by fungus Verticillium fungicola and Trichoderma harzianum, respectively was also ascertained during the present study. V. fungicola reared T. putrescentiae population on Agaricus bisporus spread the disease within 8 days culture plates and fruiting bodies. Similarly, T. harzianum reared T. putrescentiae population on Agaricus bisporus spread the green mould disease to culture plates and fruiting bodies.A peak in population (47.40, and 60.80 mites) on V. fungicola and T. harzianum was recorded after 12 and 14 days, respectively which was statistically higher than the mites recorded at other observation periods.