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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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  • ThesisItemOpen Access
    Isolation and identification of associated proteins/allergens in mite, Tyrophagus putrescentiae (Schrank) and its management
    (CCSHAU, 2016) Anita; Gulati, Rachna
    Investigations were carried out to assess the incidence of Tyrophagus putrescentiae in granaries, flour mills and domestic storage. In granaries, first peak in the month of October (12.33 mites/50g residual dust) and in flour mills and domestic storage, it was recorded in the month of November (30.06, 18.06 mites/50g wheat flour). At all the sites, second peak was recorded in the month of May (23.40, 33.26 and25.13 mites/50 g sample, respectively). Significantly more number of mites (19.00, 17.00 mites/50g grains) were recorded in open type storage (wheat grains stored on the floor of a room) than in metallic bins. Mite incidence showed significant positive correlation with maximum temperature, sunshine hours and negative correlation with evening relative humidity in granaries, flour mills and domestic storage structures. In the latter two survey sites, significant negative correlation between mite incidence and morning relative humidity was also observed. Significantly higher number of mites were recorded in flour mills (24.83 mites/ 50g sample) followed by domestic storage structures (15.84 mites/50g sample) and granaries (9.56 mites/50g sample). Maximum number of mites (64.72 mites/50g sample) were recorded during summer season (April- June), followed by winter (60.26 mites/50g sample; October- January), rainy (44.93 mites/50g sample; July- September) and spring (30.98 mites/50g sample; February-March) season. Seven unknown protein of 90 kDa, 87 kDa, 68 kDa, 50 kDa, 36 kDa, 26 kDa and 16 kDa molecular weight were isolated from T. putrescentiae body. wheat flour infested with T. putrescentiae showed a significant higher count of protein, fat acidity, free fatty acids, peroxide value whereas total antioxidant activity decreased in infested flour. The pathogenicity of Beauveria bassiana against T. putrescentiae was evaluated. Under in vitro 20 to 80.5 and 17 to 74.5 percent mortality of T. putrescentiae was recorded in Direct Spray and Treated Observation arena Bioassay, respectively with 1 × 105 to 1 × 1012 conidia ml-1 concentrations of B. bassiana. LC50 values of B. bassiana were in the range of 1.83×109 to 4.45× 1011 conidia ml-1 and LT50 values varied from 1.05 to 48.17 h for eggs, larvae, nymphs and adults in both the bioassay methods. Direct Spray Bioassay was more effective as compared to Treated Food Bioassay.
  • ThesisItemOpen Access
    Feeding potential of Tyrophagus putrescentiae Schrank (Acari: Acaridae) and its management
    (CCSHAU, 2010) Anita; Gulati, Rachna
    The studies on mean population of T. putrescentiae, percent weight loss and damage caused to grains qualitatively revealed that none of the grains was completely immune to pest infestation, however their susceptibility varied significantly (p=0.05).Susceptibility of grains/ flakes increased significantly with increase in initial infestation level and duration of infestation. Among the four foods, oat flakes was found significantly more susceptible (CD = 7.87; p = 0.05) as more number of mites (240.73 mites/5g grain) was recorded than on green gram flakes (207.26 mites/5g grain) and whole grains of oats (186 mites/5g grain). Least number of mites (173.26 mites/5g grain) was recorded from whole grains of green gram. The weight loss in oat flakes was 148.49mg/ 5g followed by loss in green gram flakes (121.40 mg/ 5g), oat grains (99.65mg/ 5g) and green gram grains (92.02 mg/ 5g) at pre count of 60 mites. Highly significant positive correlation (r= 0.93 to 0.99) was recorded at 15, 30 and 45 days between T. putrescentiae population and weight loss in oat grains. Qualitative estimation of oat flakes revealed the losses in protein contents (0.4 to 1.22%), total soluble sugars (0.5 to 1.35%), starch (0.24 to 1.38%) and non reducing sugars (3.49 to 9.97%) after 45 days of infestation. However, the reducing sugars exhibited a marked increase from 1.9 g/100 g (control) to 2.72 g/100 g flakes at 45 days of mite exposure when subjected to initial count of 20 mites/ 5g flakes. Aqueous extracts of Ocimum sanctum leaves and Glycyrrhiza glabra stems showed concentration dependent activity against T. putrescentiae in oat flakes. Among the extracts, G. glabra was effective as it provided 71.53 to 94.75 percent relative protection against T. putrescentiae whereas, O. sanctum extract caused 66 to 92 percent reductions in number at different durations.