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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Vegetable Science × Author: Jammihal, Shivanand N. × End date: 2007 × Start date: 2007 × Type: Thesis ×
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    ThesisItemOpen Access
    Effect of explants and media combinations on plant regeneration in different genotypes of tomato
    (CCSHAU, 2007) Jammihal, Shivanand N.; Rana, M.K.
    The investigation was carried out on “Effect of explants and media combinations on plant regeneration in different genotypes of tomato” in the Department of Biotechnology and Molecular Biology, Chaudhary Charan Singh Haryana Agricultural University, Hisar during the year 2006 and 2007. Development of protocols for in vitro selection would provide new advances for the production of stress tolerant cultivars in tomato. The seedling of tomato cultivars Hisar Lalima (Sel.18) and Hisar Anmol (H-24) were used as planting material in the present studies. Hypocotyls and cotyledons were used as the source of explants, which were obtained from 25 to 30 days old seedlings grown under aseptic conditions. These explants were cultured on MS medium supplemented with different concentrations of BAP and kinetin in combination with IAA and IBA 0.2 mg/l. Two media, i.e., MS basal fortified with BAP 2.5 mg/l + IAA 0.2 mg/l and kinetin 2.5 mg/l + IAA 0.2 mg/l showed the maximum shoot formation and number of shoots per explant among all 24 different media combinations. The cotyledon explants were found to be more efficient in producing shoot formation. Among 24 treatments, the MS basal containing BAP 2.5 mg/l + IAA 0.2 mg/l for hypocotyls and MS basal supplemented with kinetin 2.5 mg/l + IAA 0.2 mg/l for cotyledons was found the best for days to shoot formation, percent shoot formation and number of shoots per explant. The regenerated shoots produced roots (100%) in both the cultivars Hisar Lalima and Hisar Anmol on MS medium containing IAA 0.2 mg/l, and were successfully hardened and transferred to the screen-house conditions.