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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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  • ThesisItemOpen Access
    Biochemical and physiological changes in seed quality of Wheat [Triticum aestivum (L.) em. Thell] under different salinity levels
    (CCSHAU,HiSAR, 2019) Hemender; Mor, V. S
    The present study was planned to standardize the hydropriming protocol and evaluate the effect of different seed priming treatments on seed quality and plant growth of three wheat varieties under different salinity levels. Results revealed that mean sum of squares were significant for all the factors studied indicating sufficient magnitude of variations. The work on standardization of hydropriming indicated that volume of water and duration of soaking significantly influenced the seed performance and highest germination and vigour was observed at 12 hours soaking duration in equal volume closely followed by 8 hours with double volume and a decline was observed at 16 and 20 hours. The seedling vigour at 25°C increased as compared to 20°C level but, germination percentage showed a minor reduction at 25°C. Seed priming with saline water was found to produce a negative effect on seed quality as it showed a reduction in all the parameters with increase in salinity of the priming solution as compared to simple water. Low (2 and 4 dSm-1) and moderate (6 and 8 dSm-1) salinity adversely affect the speed of germination and early seedling growth but not showing much effect on the germination percentage, but, salinity above 8dSm-1 also decreased the percent germination. The salinity also produced detrimental effects on plant morphological development and yield. All the plant growth and yield attributing parameters reduced with rise in salinity and highest effect was shown on tillering at high salinity levels which resulted in 90% decrease in grain yield at 12 dSm-1 as compared to control. Among the genotypes, KRL 213 showed its salt tolerance potential and performed better than WH 1105 and WH 1124 at high salinity whereas, WH 1124 showed some sort of tolerance at moderate level of salt stress. Seed priming with different chemical agents proved to be beneficial under saline conditions as it improved the germination and seed vigour as compared to unprimed seed. Priming with CaCl2, salicylic acid and GA3 showed the salinity mitigating effect and highest values for most of the seed vigour parameters and plant growth and yield traits
  • ThesisItemOpen Access
    Studies on Seed Quality Enhancement and Storability in Fennel (Foeniculum vulgare)
    (CCSHAU,HiSAR, 2019-09) Suryapal Singh; Sangwan, V.P.S
    The present study entitled “Studies on Seed Quality Enhancement and Storability in Fennel (Foeniculum vulgare)” was carried out in the Laboratory of Department of Seed Science and Technology, CCS Haryana Agricultural University, Hisar for a period of 18 months during 2017-2019 under ambient conditions on cultivar Hiasr Swarup (HF 33) of fennel to ascertain the influence of seed pelleting and priming on seed quality and also the combined effect of both on the storability of fennel seeds. The present research comprised of the three different experiments, which were laid out in Complete Randomized Design (CRD) and consisting of 7 pelleting and 15 priming treatments with three replications to assess the different physiological and biochemical parameters in fennel. In the first experiment, fennel seeds responded well to different pelleting treatments. The physiological parameters (standard germination, speed of emergence, seedling length, seedling dry weight and vigour indices) and biochemical parameters (catalase, peroxidase, dehydrogenase and superoxide dismutase activity) increased on pelleting with Captan @3 g/kg + Imidacloprid@ 2g/kg followed by neem leaf powder (100 g/kg seeds), whereas, electrical conductivity was recorded minimum in above pelleting treatments. In the second experiment, priming with Trichoderma viride (8g/kg seed) or KNO3 (1%) significantly improved the physiological and biochemical parameters, which increased significantly over the control, apart from electrical conductivity. In the third experiment, the seed quality relating to physiological and biochemical traits decreased with advancement in storability except for electrical conductivity. Better retention of seed quality characters viz., standard germination, speed of emergence, seedling dry weight, vigour indices along with biochemical characters was observed in the seeds pelleted with Captan (3 g/kg) + Imidacloprid (2 g/kg) followed by KNO3 (1%) and neem leaf powder (100 g/kg seeds) after 18 months of storage (except seedling length and seed vigour index-I). The minimum EC was recorded in seeds primed with Trichoderma viride (8g/kg seed), followed by KNO3 (1%) and seeds pelleted with Captan @3 g/kg + Imidacloprid @2 g/kg. Hence, it can be concluded that in order to maintain better seed quality of fennel during storage, seed pelleting proved superior over seed priming that ought to be given as pre-sowing treatment not as pre-storage treatment.
  • ThesisItemOpen Access
    Effect of priming on seed quality and storability of maize (Zea mays L.) Hybrids
    (CCSHAU, Hisar, 2019-11) Kibaraza, Areth; Bhuker, Axay
    Seed priming is reported as a beneficial technique by researchers in many crops, in which seeds are soaked in water for limited imbibition time to allow reversible stage of germination but no protrusion of radical. Seed deterioration is a continuous process which can‘t be stopped but it can be slow down by using suitable packaging materials (containers). Hence, the study was conducted to assess the effect of priming on seed quality and storability of five maize hybrids collected from public and private seeds sectors viz., PMH-1, IIMRNH 2015-3, IIMRNH 2015-4, P3396 and Dekalb 9144 during 2018-19. The seeds were primed with double quantity of distilled water, KH2PO4 (2.0%), KNO3 (1.0%) and GA3 (50 ppm) for 17 hours at room temperature and then dried to original moisture content for 24 hours at 34.5o C temperature (average). Then the seeds were stored in polythene & cloth bags and seed quality was assessed at 3 months interval till 9 months. The results revealed that priming reduced the viability of the seed considerably but the rate at which the priming reduced the viability of seed varied with the hybrids and treatments. Reduced viability of the seed was associated with priming duration, temperature, quantity of solution and method of priming. Likewise primed seed had shorter storability, average germination of primed seed dropped below IMSCS in both containers after 3 months of storage. Dekalb 9144 and IIMRNH-2015-4 maintained average germination above IMSCS (Indian Minimum Seed Certification Standards) for 6 months in polythene bag. Moreover, KH2PO4 (2.0%), KNO3 (1.0%) maintained germination above IMSCS in Dekalb 9144 even after 9 months storage in both containers. Reduction in seed quality of primed seed might be associated with increased sensitivity of primed seeds to deteriorative factors such as temperature, oxygen and moisture content due to weakening of protective structures of the seed and reduced antioxidant enzyme activities. It is concluded from the study that priming of fresh maize hybrid seed for 17h at high temperature (Day 40oC and Night 29oC) reduces the quality of the seed. Priming technique must be standardized before its application for its beneficial effects; otherwise it may adversely affect the seed quality. Priming should be used as pre-sowing, not as pre-storage treatment. Polythene bags (with thickness >700gauge) are more suitable to maintain the seed quality as compared to cloth bags.
  • ThesisItemOpen Access
    Effect of organic seed coating on quality and storability of Mungbean (Vigna radiata L.)
    (CCSHAU, 2019) Rahul Kumar; Mor, V.S.
    The quality seed is the most important input for any crop establishment and contribute a lot for production & productivity. The availability of viable and vigorous seed at the time of planting is essential to achieve the target of agricultural production. The good quality seed act as a catalyst for recognizing the potential of other inputs. The organic agriculture boost the utility of botanicals products instead of synthetic products for sustainable growth of agriculture. Keeping in view the experiment was carried in Department of Seed Science and Technology, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana to study the effect of organic seed coating on quality and storability of mungbean (Vigna radiata L.). The seeds were treated with botanicals leaf powder @ 10gm/100gm seed and gum used as adhesive @ 2ml/ 100gm of seed. The experiments consists of eleven treatments viz., control, gum, ajwain leaf powder, neem leaf powder, sheesham leaf powder, curry leaf powder, ram tulsi leaf powder, sham tulsi leaf powder, turmeric rhizome powder, woodash, turmeric leaf powder, marua leaf powder on two varieties of mungbean i.e, MH 318 and MH 421. The present investigation was carried out in two different experiments to meet out the objectives of research. In the first experiment, both the varieties were treated just after threshing and at an interval of 6, 12 and 18 months of ageing. The six month old seed of both the varieties resulted better performance in seed quality parameters such as germination, speed of germination, seedling length, seedling dry weight, vigor index, emergence index and seedling establishment as compared to freshly treated seed. After 6 months of ageing a continuous declining trend was observed with advancement of storage period. Turmeric rhizome powder showed significant higher values for all the seed quality parameters. The earliest treated seed after threshing with various botanical powders can be maintained well in respect of better seed quality parameters and minimum insect damage during storage.
  • ThesisItemOpen Access
    Effect of organic seed coating on quality and storability of sesame (Sesamum indicum L.)
    (CCSHAU, 2019) Gagandeep Singh; Mor, V.S.
    Seed quality plays crucial role in the crop establishment and overall performance of the crop. The availability of viable and vigorous seed at the time of planting is essential to achieve the target of agricultural production. The good quality seed act as a catalyst for recognizing the potential of other inputs. The organic agriculture boost the utility of botanicals products instead of synthetic products for sustainable growth of agriculture. Keeping in view the experiment was carried in Department of Seed Science and Technology, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana to study the effect of organic seed coating on quality and storability of sesame (Sesamum indicum L.). The seeds were treated with botanicals leaf powder @ 10gm/100gm seed and gum used as adhesive @ 2ml/ 100gm of seed. The experiments consists of eleven treatments viz., control, gum, ajwain leaf powder, neem leaf powder, sheesham leaf powder, curry leaf powder, ram tulsi leaf powder, sham tulsi leaf powder, turmeric rhizome powder, woodash, turmeric leaf powder, marua leaf powder on two varieties of sesame i.e, HT 1 and HT 2. The present investigation was carried out in two different experiments to meet out the objectives of research. In the first experiment, both the varieties were treated just after threshing and at an interval of 6, 12 and 18 months of ageing. The six month old seed of both the varieties resulted better performance in seed quality parameters such as germination, speed of germination, seedling length, seedling dry weight, vigor index, emergence index and seedling establishment as compared to freshly treated seed. After 6 months of ageing a continuous declining trend was observed with advancement of storage period. Turmeric rhizome powder showed significant higher values for all the seed quality parameters which was followed by marua leaf powder shesham leaf powder, curry leaf powder and ajwain leaf powder whereas, gum alone gave the negative effect on seed quality in both varieties.
  • ThesisItemOpen Access
    Seed quality assessment in naturally aged seed of sorghum [Sorghum bicolor (L.) Moench]
    (CCSHAU, 2019) Nisha; Jakhar, S.S.
    Two sorghum variety produced during kharif seasons were evaluated for their performance for different viability and vigour parameters viz. test weight, seed density, standard germination, seedling length, seedling dry weight, vigour Index-I, vigour Index-II, tetrazolium test, electrical conductivity, dehydrogenase activity test, seed mycoflora, speed of emergence index, mean emergence time and seedling establishment (%). Significant amount of variation was observed in both of the varieties and all the seed lots for all characters. In natural aged seed lots, only HJ541 variety of sorghum sustained their germination up to one year showed that sorghum seed can be stored at ambient condition up to one year without loosening its viability and after that the germination falls below standard. HJ541 was found superior variety based on majority of the viability and vigour parameters results whereas HC136 was found inferior. During natural ageing, standard germination, seed density, test weight, seedling length, vigour indices, viability, speed of emergence index and seedling establishment decreased significantly and progressively with the ageing period. The activity of DHA decrease significantly with storage period in both the varieties. Electrical conductivity was found significantly & negatively correlated with standard germination, indicating that the more the EC value lower will be the germination in laboratory as well as in the field and vice- versa. Priming of the seeds with various treatments was found effective to improve the seed quality in fresh as well as marginal seed lot i.e. one year seed lot. GA3 (50ppm for 6 hrs.) was found better priming treatment for enhancing the quality followed by hydration- dehydration (6hrs.) + 0.25% thiram treatments. All the priming treatments showed maximum effect on HJ541 followed by HC136. Therefore, from present investigation it is concluded that the standard germination, vigour indices and EC could be used as reliable predictors of seed quality because of easy, quick and accurate in their execution. Various seed priming treatments can be used for enhancing seed quality of marginal seed lot.
  • ThesisItemOpen Access
    Effect of biostimulants to mitigate the impact of terminal heat stress on growth and yield in wheat (Triticum aestium L.)
    (CCSHAU, 2019) K.T. Uma; Sangwan, V. P. S.
    A field experiment was conducted at Department of Seed Science and Technology, CCS HAU, Hisar, Haryana to study the efficacy of bio-stimulants in mitigating terminal heat stress and also to assess effect of bio-stimulants on growth and yield in wheat. The experiment was carried out in randomized block design and replicated thrice. The experiment consisted of three date of sowing i.e., 15th November (normal sown), 1st December (late sown) and 15th December (very late sown) with two contrasting varieties, WH-711 & WH-1124 and foliar spray with eight biostimulants such as control (water), glyceine Betaine (600ppm), salicylic acid (400 ppm), cytokinin (100 ppm), ascorbic acid (10 ppm), orthosalicylic acid (20 ppm), Citric acid (1.5 %) and KCl (1 %). Biostimulants were sprayed at anthesis stage. Result shows that, grain yield was increased significantly and maximized (4.57 and 5.15 kg plot-11 for WH-711 and WH-1124 respectively) when the crop received foliar spray of glyceine betaine (600 ppm) at anthesis stage over control. The corresponding values with the foliar spray of salicylic acid (400 ppm) showed same trend like glyceine betaine and were found to be statistically at par. Variety WH-711 has performed better when it sown on normal date of sowing whereas it is second date of sowing for variety WH-1124. Application of bio-stimulants showed positive effect on length of ear, number of grains per ear and grain yield whereas plant height, effective number of tillers per plant and number of days to 75% heading remains unaffected. Variety WH-711 had higher germination percentage, shoot length and vigour index-I whereas seedling dry weight was more in late sown variety and thereby vigour index-II too. The study thus reveals that ill effects of high temperature stress on yield & yield attributes of wheat varieties may be improved by treatment with glyceine betaine (600 ppm) under normal as well as late sown conditions.
  • ThesisItemOpen Access
    Effect of seed treatments and containers on quality and storability of sorghum [Sorghum bicolor (L.) Moench] seed
    (CCSHAU, 2019) Bittu Ram; Jakhar, S.S.
    The present investigation was carried out at the Department of Seed Science and Technology, CCS Haryana Agricultural University, Hisar to assess the effect of various treatments and containers on seed quality and storability in sorghum. The seed material comprised of sorghum (variety: HJ541) were treated with 17 fungicides and eight plant oils stored in different containers viz.cloth bag and polythene bag and metal box. All the treatment combinations were evaluated for seed quality parameters viz. germination, shoot length, root length, seedling dry weight, vigour indices (I and II), electrical conductivity, dehydrogenase activity, seed mycoflora and field parameter viz. speed of emergence index, mean emergence time and seedling establishment. All the treatments showed better performance as compared to untreated control. It was found that use of carboxin + thiram (2g kg-1 seed) proved superior as compare to other fungicides and among the plant oils neem oil (2ml kg-1) perform better than others. Among containers, polythene bag proved better followed by metal box and cloth bag for storability of sorghum seed at ambient storage condition. Seed quality parameters decreased with the passage of time in all the containers and treatments. The seed quality parameter i.e. electrical conductivity and one field parameter i.e. mean emergence time showed increased values with the progress of storage period. The percentage frequency of seed mycoflora also increased with the passage of time.
  • ThesisItemOpen Access
    Seed quality assessment in natural and artificially aged seed of fenugreek (Trigonella foenum-graecum L.)
    (CCSHAU, 2019) Sunil Kumar; Sangwan, V.P.S.
    The present study entitled “Seed quality assessment in natural and artificially aged seed of fenugreek (Trigonella foenum-graecum L.)” was carried out in the Department of Seed Science & Technology at CCS Haryana Agricultural University, Hisar (Haryana). The experiments were laid out in factorial Complete Randomized Design (CRD) and Randomized Block Design (RBD) for laboratory and field parameters, respectively on six genotypes of fenugreek viz., HM-57, HM-103, HM-273, RMT-361, FGK-49, FGK-80. The present investigation was carried out in three different experiments to meet out the objectives of study. In the first experiment, To assess the seed quality during natural ageing at 3 months interval up to 18 months and check physiological and biochemical parameters viz., standard germination, seedling length, seedling dry weight, vigour index-I & II, viability test (Tz %) catalase (CAT) peroxidase (POX), superoxide dismutase (SOD), dehydrogenase enzyme activity parameters were decreased significantly with the advancement of natural ageing of seeds whereas, electrical conductivity of seeds leachates increased with the advancement of time. The field parameters viz., seedling emergence index and seedling establishment were also decreased significantly with the advancement of time whereas, mean emergence time increased with the passage of time. In the second experiment, to study the physiological and biochemical change after accelerated ageing of all six genotypes of fenugreek. Effect of artificial ageing was more as compared to natural ageing to degradation fresh seed. In the third experiment effect of priming on marginal seed of natural and artificial aged seed of all six genotypes of fenugreek. Seed priming treatments viz., T0: Control, T1: hydro-priming followed by dry dressing with thiram @ 0.25%, T2: Hydration with GA3 (50 ppm), T3: Hydration with PEG (6000), T4: Hydration with KNO3 (0.5%), T5: 2% CaCl2 were analyzed to identify the suitable priming treatment. Among various priming treatments „T2‟- hydration with GA3 @ 50 ppm followed by „T1‟-Thiram performed best to enhance all the seed vigour and viability characteristics and to lower down the electrical conductivity of naturally and artificially aged seed of fenugreek. The seed quality improvement through seed priming was noticed more in marginal seed i.e. artificial aged seed. Among the different genotypes, maximum enhancement was observed in FGK-80 followed by FGK-49 whereas, minimum enhancement was observed in genotype HM-57 during the study. In conclusion, the present study revealed that fenugreek seed lose its viability and vigour with the advancement of storage time and seed priming with GA3 @ 50 ppm and thiram can be used as an effective tool to enhance vigour and viability of seed.