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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2008 - 200912010 - 20161
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Subject: Plant Physiology × Author: Mahesh Kumar × Start date: 1986 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Morpho-physiological and biochemical traits for cold tolerance of chickpea (Cicer arietinum L.) genotypes
    (CCSHAU, 2016) Mahesh Kumar; Neeraj Kumar
    The present investigation was carried out with six chickpea genotypes viz. ICCV 88506 (Tolerant, National check), HC-1, H03-56, H07-120, H08-71 and H09- 96 differing in their cold sensitivity, to study the low temperature (<5°C) induced changes in morphological, physiological, biochemical, yield and its attributing character under field conditions. Sampling was done at 30, 60, 90 and 120 days after sowing (DAS) for growth parameters and 4 day exposure to below (stress) and above (control) 5 °C temperature for physiological and biochemical parameters. The water potential (Ψw) and osmotic potential (Ψs) of leaves decreased in all the genotypes after 4 days exposure (DAE) to low temperature (<5 °C). H03-56 showed more negative values Ψw of leaves i.e. from -0.70 to -0.85 MPa as compared to -0.45 to -0.65 MPa in HC-1, respectively in control (>5 °C) and stress (<5 °C) conditions. Likewise, the Ψs of leaves decreased from -0.84 to -1.04 MPa in H03-56 and -0.59 MPa to -0.81 MPa in HC-1. With exposure to low temperature (<5 °C), RWC (%) of leaves also declined in all the six genotypes. RWC (%) was higher in HC-1, followed by H07-120 and lowest in H03-56 in stress (<5 °C) conditions. The proline content of leaves increased significantly from 0.781 to 0.913 and 0.605 to 0.798 mg g-1 dry weight, respectively in HC-1 and H03-56 genotypes in stress (<5°C) conditions. Likewise, the total soluble carbohydrate (TSC) content of leaves also increased from 18.35 to 22.74 and 16.83 to 19.69 mg g-1 dry weight in HC-1 and H03-56, respectively. A marked increase in hydrogen peroxide H2O2, lipid peroxidation (MDA content) and relative stress injury (RSI %) was noticed in leaves which was much highest in H03-56 than all other six chickpea genotypes in stress (<5 °C) condition. More negative values of Ψw of leaves, Ψs of leaves and better accumulation of osmotically active solutes, i.e. proline, TSC of HC-1 and H07-120, helped in maintaining the higher RWC % of these organs than noticed in other genotypes in stress conditions. The specific activities of ROS scavenging enzymes such as SOD, CAT and POX also increased in leaves of all the six chickpea genotypes, in stress (<5 °C) condition. The increase was more in HC-1 and H07-120 as compared to other chickpea genotypes. Higher activities of antioxidant enzymes, lower accumulation H2O2 and MDA content in HC-1 and H07-120 than all others indicated that these enzymes play a key role in removal of ROS better in these genotypes than all other six chickpea genotypes, thus minimizing the cellular damage caused by ROS under chilling stress. Pollen viability (%) was also adversely affected in stress (<5 °C) condition; the effect being most pronounced in H03-56. The yield parameters like number of branches plant-1, number of pods plant-1, number of seeds plant-1, 100 seed weight and seed yield plant-1 were more in HC-1 and H07-120 than others in early sown condition.Seed yield had significantly positive correlation with RWC, pollen viability, proline content, total soluble carbohydrates, SOD, CAT, POX and negative with water potential, osmotic potential, H2O2 and RSI. Hence, the mechanism of cold tolerance was better in HC-1 and H07-120 than other six chickpea genotypes as found from physiological, biochemical, reproductive behavior, yield and its attributing traits studied.
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    ThesisItemOpen Access
    Transpiration traits and biodrainage potential of some tree species
    (CCSHAU, 2008) Mahesh Kumar; Datta, K.S.
    The present investigation was carried out on 10 different tree species i.e. Eucalyptus Clone-10, Eucalyptus Clone-130, Eucalyptus Clone-3, Eucalyptus hybrid, Tamarix aphylla, Prosopis juliflora, Callistemon viminalis, Melia azedarach, Terminalia arjuna and Pongamia pinnata to study various transpirational traits and correlate these with their biodrainage potential. For this, their growth parameters (height, diameter at breast height (DBH) and girth) and physiological traits (leaf area index (LAI), stomatal conductance, stomata density, relative water content (RWC), excised leaf water loss (ELWL), potometric water loss (PWL), sub stomatal CO2, transpiration rate, and photosynthesis rate) were recorded. Eucalyptus C-10, Eucalyptus C-3, Eucalyptus C-130 and Eucalyptus hybrid showed maximum plant height and DBH. LAI was highest in Eucalyptus hybrid. Stomatal density was found in the range of 197-335/mm2 on adaxial side, 215-451/mm2 on abaxial side. Minimal stomata density of 167 /mm2 was found in Tamarix aphylla and maximum of 592/ mm2 in Callistemon viminalis. Overall range of stomatal conductance, photosynthetic rate, sub-stomatal CO2 and transpiration rate were in range of 0.08-0.40 mol m2 S-1, 3.52 to 12.0 μ mol m2 S-1, 325.3- 269.48 μ mol/ mol, 4.22-7.39 m mol m2 S-1 respectively. PWL, RWC, ELWL were on the higher side in Eucalyptus species (varying amongst different species). Melia azedarach showed longer and wider vessels length than other species but Tamarix aphylla had shortest vessels and Eucalyptus Clone -130 had narrowest vessels. Tracheids were longer in Eucalyptus C-10 and shortest in Melia azedarach with wide variation among the different tree species. However, width of tracheids in all the species was in a very narrow range i.e. 20-29 μm. It was seen that water table started declining beneath the plantation after 08.00 hrs, and towards 16.00 hrs it reached a minimum level during the diurnal cycle. Amongst the different tree species biodrainage potential, as determined by decline in water table beneath the plantation, was in order of: Eucalyptus C-10 ≈ Eucalyptus hybrid > Eucalyptus C-130 ≈ Tamarix aphylla > Prosopis juliflora > Eucalyptus C-3 > Callistemon viminalis ≈ Melia azedarach > Terminalia arjuna ≈ Pongamia pinnata. Correlation analysis showed that by and large biodrainage potential had a significant positive correlation with LAI, height, DBH, stomata density and transpiration rate. Trends of significant correlation with other physiological traits like PWL, RWC, ELWL and length and width of tracheary elements were not significantly consistent in all the species.