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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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20223
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Subject: Plant Pathology × End date: 2022 × Start date: 2022 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Isolation, screening and evaluation of arbuscular mycorrhiza for the management of tomato wilt caused by Fusarium oxysporum f. sp. lycopersici
    (Chaudhary Charan Singh Haryana Agricultural University hisar, 2022-11) Sarita; Narender Singh
    Investigations were carried out on isolation and screening of arbuscular mycorrhiza on tomato wilt cause by Fusarium oxysporum f.sp.lycopersici.Samples for the investigation were collected from the tomato fields of COA, Bawal and its adjoining area and the vegetable farm of Hisar (also adjoining area) for collection of rhizosphere soils of tomato for isolation of mycorrhizal spores. Among all these samples total of 20 isolates (HM-1 to BM-10) were selected for further study. Mycorrhizal fungi were maintained on wheat (Triticum aestivum) and pearl millet (Pennisetum typhoides). In soil, the sporocarp population was highest in Glomus fasciculatum (8774), while mycorrhizal colonization was more in Glomus mosseae (96.1 %) among all the treatments. Effect of the mycorrhizal species (Glomus fasciculatum, Glomus mosseae, Glomus intraradices and Glomus hoi) and isolates (HM-1 to BM-10) were observed on growth parameters (plant height, fresh shoot weight and fresh root weight), mycorrhizal per cent colonization and sporocarp number and NPK content of tomato plants and found that all the treatments were significantly superior as compared to uninoculated control. Maximum plant height (48.4cm), shoot weight (14.63g) and root weight (4.53g) were found in isolate BM9. Hence, isolate was selected for further experiments. A negative effect was observed on the viability of different mycorrhizal species (Glomus mosseae, Glomus fasciculatum, Glomus hoi and Glomus intraradices) uncoupled from the host. After 7 months of shoot removal minimum and significant lowest viability was observed in Glomus intraradices (35.4 %) and the maximum was in Glomus mosseae (40.1 %) (2020). During 2021, minimum and significant lowest viability was observed in Glomus intraradices (32.9 %) and the highest in Glomus hoi (39.2 %). Maximum mycorrhizal population (Mycorrhizal colonization and sporocarp number), plant growth parameters (plant height, fresh weight of shoot and fresh weight of root and dry weight of shoot and dry weight of root), Mycorrhizal inoculation effect and mycorrhizal dependency was found in onion which was sequential crop after tomato as compared to without sequential crop rotation. Effect of different treatments on wilt intensity of tomato was observed in screen house conditions and maximum wilt intensity was found in Fusarium oxysporum (75.27 %) inoculation followed by Fusarium oxysporum→ Mycorrhiza (52.47 %). Wilt disease of tomato was most effectively managed (61.30 %) by combinational application of mycorrhiza, vermicompost, FYM and spent mushroom substrate mushroom substrate recession of decreased total soluble proteins and sugars and boost of total phenol was noted in F. oxysporum inoculated plants.
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    ThesisItemOpen Access
    Studies on plant parasitic nematodes associated with Kinnow in Haryana and their management
    (Chaudhary Charan Singh Haryana Agricultural University hisar, 2022-05) Sujata; Kanwar, R. S.
    Present investigations were carried out for studying plant parasitic nematodes associated with Kinnow in Haryana and their management. Survey of Hisar, Fatehabad and Sirsa districts conducted during 2019-20 and 2020-21 revealed the occurrence of four major plant parasitic nematodes viz., Tylenchulus semipenetrans, Helictylenchus dihystera and Hoplolaimus indicus in Kinnow orchards. Among them, H. dihystera had highest frequency of occurrence (90.1%) but T. semipenetrans was the most dominant species. It had 45.6, 57.1, 97.7 per cent frequency of occurrence, relative density and absolute density, respectively. In all the three districts, H. dihystera had the highest frequency i.e., 72.4, 80, 70.3 per cent in Hisar, Fatehabad and Sirsa, respectively. Citrus nematode had maximum frequency (62.9 %) form Sirsa district. Correlation of T. semipenetrans with electrical conductivity (r = 0.07NS), soil pH (r = 0.22 NS), soil organic matter (r = 0.21 NS) and orchard age (r = 0.30 NS) were non-significant. Correlation between Pratylenchus coffeae and ecological factors were also non singnificant i.e., for EC (r=0.23), soil pH (r = 0.30), soil organic matter (r = -0.22), orchard age (r = 0.24). Population of H. dihystera showed no correlation with soil organic matter, weak positive correlation with EC (r = 0.17NS) and pH (r = 0.14NS) and moderate positive correlation with orchard age (r = 0.38NS). Similarly, H. indicus correlations with ecological factors were non-significant. Seasonal fluctuation and vertical distribution of plant parasitic nematodes associated with Kinnow was studied at four locations viz., Horticulture research farm, CCS HAU, Hisar, Malapur, Nangthala and Arya nagar. Maximum number of phytonematodes were recorded in November (2020) followed by March (2021) and lowest in July (2021). More number of nematodes were present at 15-20 cm soil depth as compared to 21-30 cm and 31-45 cm depth. Experiments on management were done at Malapur during 2020-21 and at Horticulture research farm, CCS HAU, Hisar during 2021-22. Application of neem cake @ 1.5 kg, T. viride @ 200 g and neem cake @ 1 kg with T. viride @ 100 g per plant significantly reduced the nematode population and increased the yield significantly over control.
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    ThesisItemOpen Access
    Occurrence and variability of Magnaporthe grisea inciting blast disease of pearl millet [Pennisetum glaucum (L.) R. Br.]
    (Chaudhary Charan Singh Haryana Agricultural University hisar, 2022) Pankaj; Vinod Kumar
    The present study entitled, ―Occurrence and variability of Magnaporthe grisea inciting blast disease of pearl millet [Pennisetum glaucum (L.) R. Br.]‖ was carried out in laboratory and screen house Department of Plant Pathology, CCS Haryana Agricultural University, Hisar. The study was aimed to know the occurrence and severity of pearl millet blast disease in Haryana, morphological, molecular and pathogenic variability among M. grisea isolates and chemosensitivity of different isolates of M. grisea. The maximum pearl millet blast incidence and severity was in Bhiwani district followed by Mahendragarh district whereas, minimum was in Nuh district during both the years. All the collected M. grisea isolates showed a considerably variation in cultural and morphological characters and pathogenic behaviour. Among all the isolates the maximum radial growth 42.00 mm was recorded in isolate Mg 12 followed by Mg 10 (41.67mm) and Mg 11 (41.33 mm) whereas, the minimum radial growth of 22.00 mm was recorded for Mg 4 isolate. The maximum average length of the spores (35.21 μm) was also observed in Mg 12 isolate which is significantly higher among all the isolates followed by Mg 10 (33.49 μm) and Mg 11 (31.58 μm). Mg 4 isolate (22.98 μm) showed a minimum average length of spores. Among all the fungal isolates Mg 4 isolate was found to be least pathogenic with 39.75 per cent disease severity followed by isolate Mg 6 (40.00%). The isolates Mg 12 (60.29 %), Mg 11 (57.51 %) and Mg 10 (56.84 %) were recorded most pathogenic. All the isolates showed high level of polymorphism. The cluster analysis based on ISSR primers the isolate Mg 4, Mg 6 and Mg 16 were most diverse and isolates Mg 10 and Mg 12 had the highest similarity coefficient (1.00) among the all 16 M. grisea isolates. The high relative humidity more than 90 %, Temperature 25-30°C and slightly acidic pH (6.0) favours the growth and sporulation of Magnaporthe grisea under in vitro conditions. Among the fungicides evaluated propiconazole reported maximum inhibition of the mycelial growth (91.48 %) of the fungus followed by Tebuconazole as it showed 76.17 per cent growth inhibition. The both the chemicals were significantly superior over carbendazim (68.21 %) which was found to be the least effective in inhibiting mycelial growth of the fungus.