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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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20192
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Subject: Plant Pathology × End date: 2019 × Start date: 2019 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Investigations on endophytic bacteria for management of downy mildew caused by Sclerospora graminicola in pearl millet
    (CCSHAU, Hisar, 2019-12) Sangwan, Pooja; Kushal Raj
    Pearl millet is a valuable staple food in semi-arid regions of the world. Pearl millet is affected by many diseases among them, downy mildew incited by oomycetes pathogen Sclerospora graminicola (Sacc.) Schroet is the most widespread and destructive disease of pearl millet. Endophytes play an important role in metabolism and physiology of the host plants. They provide multifarious benefits such as growth promotion, suppression of pathogens and nitrogen fixation. In the present study, roots and stubbles were collected from sick plot area of Bajra Pathology after the harvest of the pearl millet crop during Kharif 2016 and 2017. A total of 30 pearl millet root bacterial endophytes (PMRBE1- PMRBE 30) were retrieved from crushed roots of pearl millet streaked on Nutrient agar plates. Ten endophytic bacterial isolates were retrieved from roots of kharif 2016 crop and other twenty isolates from roots of kharif 2017 crop. All endophytic bacterial isolates were assessed for biocontrol activities. Amongst 30 pearl millet root bacterial endophytes, sixteen isolates were positive for siderophore production, three isolates viz. PMRBE6, PMRBE25 and PMRBE28 were found positive for HCN production, 30% of the bacterial endophytes showed good growth on ACC supplemented plates and cellulolytic activity was found in bacterial endophyte PMRBE18. The isolates PMRBE1, PMRBE4, PMRBE5, PMRBE6, PMRBE7 PMRBE17, PMRBE18, PMRBE20, PMRBE23 and PMRBE24 which showed maximum germination, seedling vigour index I & II and germination speed of three pearl millet cultivars HHB226, HHB67 Imp and 7042 S under in vitro conditions were selected for seed treatment as well as for foliar spray under screen house and field conditions. Amongst various pearl millet root bacterial endophytes, maximum germination, minimum downy mildew incidence at 30 and 60 DAS, better growth and yield of pearl millet cultivars were found on treatment with isolate PMRBE6, PMRBE18 and PMRBE23. On the basis of biochemical characterization and partial 16S rRNA sequencing pearl millet root bacterial endophytic isolates PMRBE6, PMRBE18 and PMRBE23 were identified as Bacillus subtilis strain PD4 (Accession no. MN400209), Serratia sp. and Alcaligenes faecalis strain HX2016003 (Accession no. MN400208). Promising root endophytes may be used for IPM of downy mildew in pearl millet after field trials.
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    ThesisItemOpen Access
    Variability, epidemiology and management of Tilletia indica Mitra, the incitant of Karnal bunt of wheat
    (CCSHAU, Hisar, 2019-11) Mamta; Rajender Singh
    Karnal bunt disease of wheat continues to be a potential threat to production and trade of wheat. The present study was planned to study cultural, morphological, molecular and pathogenic variability in Tilletia indica, epidemiology and management of this disease. On the basis of cultural and morphological characteristic, the largest teliospore size was observed in Bhiwani isolate, whereas Kapurthala isolate was having the smallest size. Different Karnal bunt isolates colony colour were white, yellowish white, creamy, creamish yellow and creamish white whereas, culture colony growth pattern of isolates were erect, compact, clustering, crustose, appressed, spreading and leathery. Maximum teliospore germination, primary sporidia formation and secondary sporidia production was recorded at 18°C in Pantnagar isolate. Best teliospore germination occurred at pH 6.0 followed by pH 5.0 Maximum coefficient of infection was expressed in HD2967 followed by DPW 621-50. Pantnagar isolate exhibited maximum mean coefficient of infection followed by Bhatinda and the least was observed in Mahendergarh isolate. Pantnagar isolate was most virulent as well as had maximum sporulation. Karnal bunt resistant reaction in three differentials namely HD 29, HD 30 and Aldan were noted. In molecular variability twelve molecular markers were tested against 10 Karnal bunt isolates and characterized into 3 major groups. Karnal bunt incidence had significant correlation with maximum temperature, rainy day and total rainfall during earhead emerging stage Two hundred seventy nine genotypes showed less than 5 coefficient of infection against Karnal bunt thereby expressing resistant reaction and 221 genotypes were susceptible having more than 5 per cent coefficient of infection under artificial inoculation condition. Maximum disease control was obtained by propiconazole 25% EC (0.1%) application, followed by combination of propiconazole 25% EC (0.05%), neem seed kernel and Trichoderma harzianum.