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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Plant Pathology × Author: Abhishek Kumar × End date: 2023 × Start date: 2020 ×
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    ThesisItemOpen Access
    Studies on management of ashwagandha wilt and host-microbiome interaction with its incitant (Fusarium spp.)
    (CCSHAU, Hisar, 2023-01) Abhishek Kumar; Kushal Raj
    Wilt caused by Fusarium solani is major disease of ashwagandha in India leading to significant losses. Management of disease using bacterial endophytes and arbuscular mycorrhizal fungi is considered an eco-friendly and viable alternative to synthetic fungicides. The present study was carried out to explore the efficacy of bacterial endophytes retrieved from ashwagandha roots and arbuscular mycorrhizal fungal (AMF) cultures for Fusarium wilt management. Amongst 32 bacterial endophytic isolates screened against Fusarium solani under in vitro conditions, three isolates identified as Pseudomonas putida, Pseudomonas aeruginosa and Pseudomonas sp. Strain HH170 exhibited significantly higher antagonistic activity i.e. 89.06%, 86.76% and 83.09% respectively against F. solani. Under screen house and field conditions all three promising endophytic bacteria were also found promising for growth attributes as well as managing wilt of ashwagandha. Amongst different treatments, seed treatment with combination of Carboxin 37.5% + Thiram 37.5 WS @ 4 g/kg. seed followed by endophytic bacterial culture suspension (108 cells/ml) @5 ml/kg seed exhibited maximum plant disease management under both screen house and field trials. Amongst four AMF cultures, i.e., Glomus mosseae, Glomus fasciculatum, Glomus intraradices and Glomus hoi inoculation with Glomus mosseae @ 600 spores/kg soil was found best for wilt incidence reduction, plant growth enhancement as well as boosting the nutrient content in ashwagandha roots. On the basis of various experiments, it was found that the combination of Carboxin 37.5% + Thiram 37.5 WS @ 4 g/kg. seed followed by application of endophytic bacterial culture suspension (108 cells/ml) @5 ml/kg seed and Glomus mosseae @600 spores/kg soil could be used as eco-friendly and viable option for wilt management and growth promotion in ashwagandha.