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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Nematology × End date: 2019 × Start date: 2019 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Efficacy of rhizospheric bacteria for the management of rice root-knot nematode, Meloidogyne graminicola in rice nursery
    (CCSHAU, 2019) Shweta; Anil Kumar
    Research work was carried out on the efficacy of rhizosphecric bacteria against rice root-knot nematode, Meloidogyne graminicola in rice nursery using in vitro and screen house conditions. In vitro effect of native rhizobacterial strains for the nematoxicity against hatching and juvenile mortality of Meloidogyne graminicola at different concentration level i.e. S/2 and S/4. In intact bacterial culture, maximum and significantly highest larval mortality was observed, where M. graminicola larvae was exposed to Pseudomonas putida followed by Providencia rettgeri, Vibrio sp., Pseudomonas fluorescens and Bacillus sp.in S/2 and S/4 concentration. Among all the bacterial cultures, P. putida showed highest mortality at both concentrations and more mortality rate was found atS/2 concentration. All the other intact bacterial culture and CFCs significantly increased the larval mortality at both the concentrations as compared to untreated check.Effects of the different intact bacterial culture and CFCs on hatching behavior of rice root-knot nematodeat different concentration level i.e. S/2 and S/4 showed that all bacterial culture were observed to inhibit the egg hatching as compared to the untreated check. Maximum inhibition was observed in Bacillus sp.followed by to P. putida and P.fluorescens at S/2 concentration.The rate of hatching was inversely proportional to concentration of the strains at exposure period as it decreased with the increase in the concentration. The interaction between exposure period and isolates was found significant. Under screen house condition, plant growth parameters (plant height, fresh plant weight and dry plant weight) maximum and significantly high plant growth in P. putida treated plants as compared to untreated check (infested soil). The minimum plant growth parameters were observed in untreated check in soil drenching and seed soaking modes of application of rhizobacterial strains. Minimum and significantly lowest number of nematode reproduction parameters (number of galls/10 seedlings, number of eggs/10 seedlings and FNP/200cc soil) were obtained in P. putida as compared to untreated check in both methods of application. Present study revealed that management of M. graminicolaby using rhizobacteria is feasible, economical and environmentally safe in rice nursery and also proved effective component of nematode management in rice nursery.
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    ThesisItemOpen Access
    Studies on interaction between Heterodera avenae and Urocystis agropyri causing flag smut in wheat
    (CCSHAU, 2019) Sharma, Lochan; Kanwar, R.S.
    Present study was conducted to investigate the effect of different soil types on interaction of Heterodera avenae and Urocystis agropyri, the effect of sowing times on interaction between H. avenae and U. agropyri and the effect of different nematode inoculum levels on interaction of H. avenae and U. agropyri. The experiments were conducted in screen house of Department of Nematology, CCSHAU, Hisar during Rabi-2018. For evaluating the effect of different soil types on interaction between H. avenae and U. agropyri wheat cultivar HD2967 was sown in 1 kg earthen pots and observations of plant growth parameters, disease incidence and nematode population were recorded at crop maturity. Chlorophyll was measured by SPAD meter. Plant growth parameters and chlorophyll was minimum in H. avenae and U. agropyri simultaneous inoculation in loamy sand soil. There was no effect on germination and tillering by either of the pathogen. Final cyst population was more in loamy sand soil and in presence of fungus nematode population was decreased. For comparing the effect of different sowing times, wheat was sown in first week of November, last week of November and second week of December. Maximum reduction in plant growth parameters and chlorophyll was observed in simultaneous inoculation of nematode and fungus in last week of November. Maximum reduction of chlorophyll was observed 60 days after sowing in N+F as compared to uninoculated control. There was no effect of either pathogens alone or in combination on germination and tillering. Maximum cyst population was recorded in last week of November sown plants. In the experiment on effect of different inoculum levels viz. 5, 10 and 15 eggs and J2/g soil of H. avenae and constant level of U. agropyri 20g/kg seed on plant growth parameters and chlorophyll content, plant height, weight and chlorophyll were minimum in simultaneous inoculation of at 15 eggs and J2 of nematode with fungus. Maximum cysts were obtained at highest inoculum of H. avenae i.e. 15 eggs and J2/ g soil. In presence of fungus, cyst population was decreased.
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    ThesisItemOpen Access
    Effect of growing seasons on the development and pathogenicity of Meloidogyne javanica in okra (Abelmoschus esculentus)
    (CCSHAU, 2019) Deepak Kumar; Verma, K.K.
    Investigation were carried out to study the effect of different growing seasons on the development and pathogenicity of Meloidogyne javanica in okra (Abelmoschus esculentus) under screen house conditions. In development and life cycle experiments, the penetration of M. javanica was observed at initial 24 hrs of inoculation in summer and monsoon season as compared to three days of spring season. The total life cycle of M. javanica was completed in 35 days during spring season at mean temperature range between 15.4 to 33.20C and mean relative humidity 34.0 to 69.7 per cent. During summer season, it was completed in 27 days with mean temperature range of 25 to 410C and relative humidity of 39 to 67 per cent. whereas during monsoon season, M. javanica took 31 days to complete its one generation in okra crop under mean temperature range of 26 to 330C and relative humidity of 73 to 90 per cent. The experiments of pathogenicity were also carried out to study the effect of different growing seasons on the plant growth parameters as well as nematode reproduction and multiplication. With increase in inoculum levels from 10 to 10000 J2/kg soil, there was significant reduction in all growth parameters. But the inoculum level of 1000 J2/kg soil was observed pathogenic during spring and summer season while, during monsoon season, 100 J2/kg soil was found pathogenic level to okra crop. The nematode reproduction and multiplication factors was observed in increasing trend with increase in inoculum levels of nematode under all the three seasons. The reproduction factor was observed negatively correlated with increasing inoculum level of nematodes. At 10000 J2/kg soil, minimum reproduction factor was observed. The prevailing temperature during all seasons and soil moisture during monsoon season due to higher rainfall were crucial factors for development, reproduction and multiplication.