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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2009 - 200912010 - 20141
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Subject: Molecular Biology and Biotechnology × Author: Gupta, Meenu × Start date: 1990 ×
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    ThesisItemOpen Access
    Expression profiling of soluble starch synthase I gene during grain development for thermotolerance in wheat (Triticum aestivum L. em. Thell)
    (CCSHAU, 2014) Gupta, Meenu; Dhillon, Santosh
    The four genotypes of wheat i.e. two thermotolerant (WH 730 and WH 1021) and two thermosensitive (WH 147 and WH 711) were evaluated under two different sowing conditions (normal and late sown) to assess the effect of high temperature (HT) stress on grain yield traits and expression of soluble starch synthase I gene. Gene expression was studied by real time PCR analysis at 7 stages (2, 5, 8, 10, 15, 20 and 25 DAA) during grain development in developing grains and flag leaf. High temperature stress negatively affected all yield traits like number of tillers per plant, grain number per plant, thousand grain weight, biomass and grain yield per plant. Decreased grain filling duration under HT stress was not compensated by increased grain filling rate. The effect was more profound in case of thermosensitive genotypes. The order of thermotolerance based on heat susceptibility index (HSI) is: WH 730 (0.65) > WH1021 (0.79) > WH 711 (1.13) > WH 147 (1.52). In developing grains, temporal pattern of SSSI gene expression was altered and expression peaked earlier under LS conditions (15 DAA) than under NS conditions (at 20 DAA) in all genotypes studied. In flag leaf, peak expression under NS conditions was observed at 15 DAA in all genotypes while under LS conditions, expression peaked at 15 DAA in thermotolerant genotypes and at 10 DAA in thermosensitive genotypes. At late stages during grain development, SSSI gene expression fell more abruptly and to a higher level in case of thermosensitive genotypes in grains as well as flag leaf. Partial SSSI cDNA was sequenced from WH 730 (1916 bp), WH 1021 (1924 bp), WH 147 (1925 bp) and WH 711 (1924 bp) genotypes. Phylogram generated using different starch synthases isoforms clustered SSSI, SSSII, SSSIII and SSSIV into separate groups. WH 730 showed maximum tolerance to HT stress while WH 147 was least thermotolerant, based on the overall picture of physiological traits, yield traits and gene expression analysis studies.
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    ThesisItemOpen Access
    Polymorphism among thermotolerant and sensitive genotypes of wheat (Triticum aestivum L.) using DNA markers
    (CCSHAU, 2009) Gupta, Meenu; Dhillon, Santosh
    Wheat is one of the most important staple food crops and is cultivated throughout the world. High-temperature stress is one of the major constrains to wheat production world wide. This study was undertaken with the objective to assess polymorphism among 10 thermosensitive and 10 thermotolerant genotypes of wheat. DNA extracted from young leaves of 20 wheat genotypes was amplified by using 20 ISSR and 25 RAPD primers. Out of these primers, 11 ISSR and 18 RAPD primers were showing amplification and were selected for present investigation. For ISSR and RAPD assays, data was analyzed to calculate various parameters such as the number of total bands, number of polymorphic bands, per cent polymorphism, bands per primer, polymorphic bands per primer, similarity matrices and dendrogram. Both RAPD and ISSR generated a moderate level of average percentage of polymorphism i.e. 60.3% and 48.4% respectively. The ISSR primers yielded average 8.64 bands per primer while RAPD primers amplified average 7 bands per primer. The average number of polymorphic bands was higher in case of RAPDs (4.22) as compared to that in ISSRs (4.18). Overall size of PCR amplified products ranged between 220 bp and 3500 bp for ISSR primers and between 280 bp and 4000 bp for RAPD primers. Based on ISSR similarity matrix data, the value of similarity coefficient ranged from 0.69 to 0.94 with an average genetic similarity of 0.81. RAPD similarity matrices between different genotypes ranged from 0.63 to 0.89 with average similarity coefficient of 0.78. Dendrograms generated using RAPD and ISSR markers separated genotypes into two major clusters which were further divided into sub clusters. However, dendrogram based on RAPD markers was not in accord with dendrogram based on ISSR markers.ISSR-41 primers amplified a ≈2800 bp band which was present in all the 10 thermotolerant genotypes and absent in all thermosensitive genotypes except one (genotype S5). The marker identified using ISSR-41 primer may probably be thermotolerance specific and may have potential for use in marker assisted selection programs for wheat production improvement.