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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Molecular Biology and Biotechnology × Author: Gupta, Meenu × End date: 2009 × Start date: 2009 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Polymorphism among thermotolerant and sensitive genotypes of wheat (Triticum aestivum L.) using DNA markers
    (CCSHAU, 2009) Gupta, Meenu; Dhillon, Santosh
    Wheat is one of the most important staple food crops and is cultivated throughout the world. High-temperature stress is one of the major constrains to wheat production world wide. This study was undertaken with the objective to assess polymorphism among 10 thermosensitive and 10 thermotolerant genotypes of wheat. DNA extracted from young leaves of 20 wheat genotypes was amplified by using 20 ISSR and 25 RAPD primers. Out of these primers, 11 ISSR and 18 RAPD primers were showing amplification and were selected for present investigation. For ISSR and RAPD assays, data was analyzed to calculate various parameters such as the number of total bands, number of polymorphic bands, per cent polymorphism, bands per primer, polymorphic bands per primer, similarity matrices and dendrogram. Both RAPD and ISSR generated a moderate level of average percentage of polymorphism i.e. 60.3% and 48.4% respectively. The ISSR primers yielded average 8.64 bands per primer while RAPD primers amplified average 7 bands per primer. The average number of polymorphic bands was higher in case of RAPDs (4.22) as compared to that in ISSRs (4.18). Overall size of PCR amplified products ranged between 220 bp and 3500 bp for ISSR primers and between 280 bp and 4000 bp for RAPD primers. Based on ISSR similarity matrix data, the value of similarity coefficient ranged from 0.69 to 0.94 with an average genetic similarity of 0.81. RAPD similarity matrices between different genotypes ranged from 0.63 to 0.89 with average similarity coefficient of 0.78. Dendrograms generated using RAPD and ISSR markers separated genotypes into two major clusters which were further divided into sub clusters. However, dendrogram based on RAPD markers was not in accord with dendrogram based on ISSR markers.ISSR-41 primers amplified a ≈2800 bp band which was present in all the 10 thermotolerant genotypes and absent in all thermosensitive genotypes except one (genotype S5). The marker identified using ISSR-41 primer may probably be thermotolerance specific and may have potential for use in marker assisted selection programs for wheat production improvement.