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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Molecular Biology and Biotechnology × Author: Asha Rani × Start date: 2014 ×
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    ThesisItemOpen Access
    Marker assisted selection for drought tolerance in pearl millet (Pennisetum glaucum (L.) R. Br.)
    (CCSHAU, 2014) Asha Rani; Yadav, Ram C.
    Pearl millet (Pennisetum glaucum (L.) R. Br.) is the staple cereal of millions of poor rural families in the hottest and driest areas of the tropics and subtropics. Terminal drought stress is a regular occurrence and serious environmental constraint in these regions, making stress tolerance an essential attribute of new pearl millet cultivars. Marker assisted selection is an important molecular tool for improving yield and yield stability of the pearl millet. A number of genomic regions were found to be associated with drought tolerance in terms of both grain yield and its components. A major QTL associated with the drought tolerance of grain yield mapped on linkage group 2 and explained up to 32% of the phenotypic variation. Another QTL were observed on LG 5 with phenotypic variation of 14.8%. During the present investigation, HHB 226 pearl millet hybrid was improved for drought tolerance by marker assisted selection. For this, two crosses were made i.e. HBL 11 x PRLT 2 and HBL 11 x 863 B. PRLT 2 and 863 B were used as donor parent and HBL 11 (male parent of HHB 226) was used as recurrent parent. Eleven SSR primers specific to drought tolerance from LG2 and 5 were used for polymorphism analysis between recurrent and donor parent. Three SSR primers from LG 2 (PSMP2066, PSMP2077, and PSMP2059) and one from LG 5 (PSMP2078) were found polymorphic in both the crosses which were used for further screening. Recurrent and donor parents were crossed to raise the F1 generation. Selected plants were backcrossed with HBL11 three times and in each generation positive plants were selected. Finally, four plants were selected in BC3F1 generation in both crosses which were then used for background selection. 25 and 32 background primers were found polymorphic out of 64 primers in HBL 11 x PRLT 2 and HBL 11 x 863 B cross respectively. About 70 and 80% of the recurrent parent genome was found to be transferred in most of the selected plants in 1st and 2nd cross respectively till BC3F1 generation. During the drought screening test in BC2F1 generation in 1st cross, RWC and 1000 seed weight were found to be enhanced with 27.84% and 15% respectively, in drought tolerant BC2F1 generation plants than drought sensitive. In 2nd cross, RWC and 1000 seed weight were increased with 33.6% and 28% respectively, in drought tolerant plants as compared to drought sensitive.