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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2004 - 200945
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Subject: Microbiology × End date: 2009 × Start date: 2004 × Type: Thesis ×
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Now showing 1 - 9 of 45
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    ThesisItemOpen Access
    A Study of Outliers and Influential Observations in Linear Regression
    (College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 2004) Tonk, Devinder Singh; Singh, Umed
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    ThesisItemOpen Access
    Indole acetic acid production by Pseudomonas species and its effect on plant growth of green gram and black gram
    (CCSHAU, 2004) Om Prakash; Sindhu, S.S.
    In the present studies, IAA producing Pseudomonas strains CPS59 and MPS90 were mutagenized with transposon Tn5 using E. coli strain S17-1. The frequency of mutants with low amount of IAA production varied from 35.14 to 46.75% from Pseudomonas strains CPS59 and MPS90, respectively and only 3.43 to 3.75% mutants produced higher levels of IAA in comparison to respective parent strains in LB medium supplemented with tryptophan. Inoculation of mutants derived from Pseudomonas strain CPS59 showed stunting effect on root development of green gram seedlings at both 5 and 10 days of observation whereas mutants CPS59-138, CPS59-162 and CPS59-231 showed stimulatory effect on root growth at 5 days in black gram. Majority of the mutants caused stimulation of shoot growth at both 5 and 10 days of observation in comparison to uninoculated treatment. MPS90-derived mutants i.e. MPS90-39, MPS90-157, MPS90-102 and MPS90-106 showed slight stimulation of root growth whereas most of the mutants showed stunting effect on shoot growth at 10 days in black gram. Coinoculation studies of Pseudomonas mutants with Bradyrhizobium sp. strain S24 resulted in increased nodule number, nodule fresh weight and shoot dry weight in green gram and black gram under chillum jar conditions. Shoot dry weight gains in green gram after coinoculation with CPS59-derived mutants varied from 107-149 per cent and from 110-137 per cent with MPS90-derived mutants in comparison to Bradyrhizobium-inoculated plants at 60 days of growth. Similarly, shoot dry weight gains in black gram varied from 102-178 per cent and 105-198 after inoculation with mutants derived from CPS59 and MPS90, respectively. The stimulation effect on shoot dry weight in comparison to uninoculated control treatment varied from 280-390 per cent in green gram and 179-357 per cent in black gram. Four mutants CPS59- 138, CPS59-321, MPS90-133 and MPS90-51 showed more enhancements in nodule formation by Bradyrhizobium strain S24 in green gram whereas mutants CPS59-162, CPS59-64, MPS90- 102 and MPS90-280 caused more stimulation for nodule formation in black gram at 60 days of plant growth. Significant enhancements in shoot dry weights of green gram were observed by coinoculation with Pseudomonas mutants CPS59-138, CPS59-231, CPS59-321, MPS90-280, MPS90-14 and MPS90-145. Similarly, coinoculation of Bradyrhizobium strain with Pseudomonas mutants CPS59-162, CPS59-321, CPS59-214, MPS90-102, MPS90-280 and MPS90-51 showed significant gains in shoot biomass of black gram at 60 days of plant growth. The better performance of IAA over producing mutants in relation to nodulation and plant biomass indicated that IAA production by Pseudomonas strains is a beneficial trait for selection of rhizobacterial strains having plant growth promotion ability.
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    ThesisItemOpen Access
    Development of quality assurance method for biofertilizers using LACz marker
    (CCSHAU, 2004) Manoj kumar; Gera, Rajesh
    Biofertilizers are living microorganisms used to supplement nutritional requirement of plants. Strains of Azotobacter, Rhizobium, Azospirillum, Pseudomonas, Bacillus and Acetobacter have been developed as biofertilizers for various crops and are suppled as carrier based inoculants. The responses of biofertilizers are inconsistent and generally related to the poor quality and poor competitive ability of the inoculants. The quality control methods include standard plate count and most probable number (MPN) by plant infection. Both these methods are not very specific and require at least 10-30 days. Thus, the quality control methods are not strictly followed because producers are not willing to hold their product for 30 days and thus substandard biofertilizers are being supplied to the farmers. Thus, there is an urgent need to develop simple method for quality assurance of biofertilizers, which could be completed with in 6-12 hours. lacZ marked strains of Azotobacter (A-2), Rhizobium (T-19CL and PP1021L), Pseudomonas (P-35) and Bacillus (BPL-2) showed relationship between -galactosidase activity with viable cell number at different stages of growth in liquid medium. The Z buffer with 0.1% Triton X-100 was found to be the best diluent for enumeration of viable cell population in charcoal based inoculants. There was relationship in -galactosidase activity and viable cell number of all these above strains in charcoal based inoculants, which remained stable up to three months. Time for development of yellow colour of ONPG filter paper discs was correlated with viable cell number, thus providing the qualitative method for enumeration of viable cell number in the bioinoculants.
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    ThesisItemOpen Access
    Impact Of Sewage Sludge Application On Soil Microbiological Properties And Plant Growth
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2007) Walia, Meenu; Goyal, Sneh
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    ThesisItemOpen Access
    Biochemical And Molecular Diversity Among Azotobacter Spp. Under Cotton-Wheat Cropping System
    (Chaudhary Charan Singh Haryana Agricultural University;Hisar, 2005) Bhatia, Ranjana; Narula, Neeru
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    ThesisItemOpen Access
    Effect Of Genetically Marked Mesorhizobium Ciceri On Soil Bacterial Community Structure
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2009) Anju Kumari; Kapoor, K K
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    ThesisItemOpen Access
    Molecular Diversity Among Diazotrophs In Arid Zone Soils Of Haryana
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2005) Sarita; Sharma, P. K.
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    ThesisItemOpen Access
    Development Of Liquid Incoculant Technology For Supply Of Biofertilizers And To Assess Its Efficacy On Crop Productivity
    (Chaudhary Charan Singh Haryana Agricultural University;Hisar, 2004) Jitender Kumar; Anand, R. C.
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    ThesisItemOpen Access
    Characterization And Genetic Improvement Of Bacteria For Alkaline Protease Production
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2009) Putatunda, Chayanika; Kundu, B. S.