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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Microbiology × Author: Sachin × End date: 2019 × Start date: 2019 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Characterization of thermotolerant rhizobacteria to control root rot disease in clusterbean (Cyamopsis tetragonoloba L.)
    (CCSHAU, 2019) Sachin; Pathak, D.V.
    Cluster bean [Cyamopsis tetragonoloba (L.) Taub.] is an under exploited leguminous crop which is commonly known as guar, chavli kayi, guari, khutti etc. It is grown in India, especially in South Haryana, Rajasthan, Andhra Pradesh and Maharastra in the summer season. India contributes around 80% of cluster bean to the world. Rhizoctonia solani Kuhn, a basidomycetes fungus, is the main causal organism of this disease. The control of fungus is difficult because of wide range of its host, large sclerotia are insensitive to chemical fungicides. A total of fifty eight bacterial isolates were retrieved from rhizosphere of mainly two crops (Wheat and congress grass). All the bacterial isolates were screened for Gram’s staining, spore staining, IAA production, NH3 excretion, growth on chitin as carbon source, antifungal activity against R. solani, Germination index, pre emergence mortality and Relative root elongation index (RREI). On the basis of Gram’s staining 45 isolates were found to be Gram’s +ve, remaining were Gram nagetive. Endospore staining showed 45 isolates as spore formers. A total of 56 isolates were capable of IAA production and maximum IAA producer was NAA4 (25.03 μg ml-1). Out of 58 isolates 53 isolates were capable of ammonia excretion and maximum NH3+ excretion was 4.16 μg ml- 1 by isolate no. KMI1. Only five isolates (KMI1, NAB1, NAE2, NAD1 and NAC2) were showed chitinase activity using chitin as carbon source. Ten bacterial isolates were showed antifungal activity against R. solani under in vitro conditions. Impact of 20 best cultures selected on the basis of biochemical characterization, was studied for germination in cluster bean under in vitro conditions. The maximum root length was observed with isolate NAG6 (7.86 cm) and maximum shoot length was 8.82 cm with NAG6. Maximum RREI % was observed with NAG6 which was 242.59 %. Minimum pre emergence mortality was observed with KME2. It was only 3 %. Germination index varied from 50 to 90 % and maximum GI was 90% in five cultures KMB7, KMH3, KML2, NAB1, NAD1. The best five cultures on the basis of antifungal activity, chitinase activity and other biochemical characterization were selected to evaluate under pot house conditions. Maximum root length was observed with NAE2 along with RDF (18.11cm) and shoot length was observed with NAE2 along with RDF (20.76 cm). Maximum GI was observed in case of NAE2 along with RDF (82.96 %). Minimum pre emergence mortality % observed was 8.00 % in NAE2 along with RDF. Minimum post emergence mortality % observed was 9.04 % in NAE2 along with RDF. The maximum dry shoot weight was observed in case of NAE2 along with RDF (5.075 mg). The maximum dry root weight was 0.403 mg in NAE2 along with RDF followed by 0.392 mg in NAD1 along with RDF. The maximum N uptake was observed with NAE2 along with RDF (64.75 mg/pl.). NAE2 culture performed better for root rot control and most of plant growth parameter under pot house conditions. This culture was identified as Bacillus subtilis subtilis using 16sRNA RFLP.