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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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20201
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Subject: Microbiology × Author: Rana, Barkha × End date: 2020 × Start date: 2020 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Studies on molecular mechanisms underlying rhizobia promiscuity in different Khariflegumes
    (CCSHAU, Hisar, 2020) Rana, Barkha; Gera, Rajesh
    Mungbean, mothbean, cowpea, clusterbean, urdbean and pigeonpea are economically important kharif legumes commonly grown in rain-fed areas particularly South-West Haryana and Rajasthan state. It is possible to improve its symbiotic property for different kharif legumes through identification and manipulation of genetic factors that controls the promiscuity of Rhizobium. So the present investigation was undertaken to study molecular mechanism underlying rhizobia promiscuity in different kharif legumes. A total of 10 rhizobial isolates along with three rhizobial mutants were obtained from Molecular Microbiology laboratory of Microbiology Department that were retrieved from arid and hyper-arid zones of South-West Haryana and Rajasthan state. All selected rhizobial isolates were checked for their authenticity in six different kharif legumes after 45 days of growth under sterilized conditions. Promiscuous rhizobial mutants (PKR177m30, PKR188m85 and PKR194m93) have the ability to nodulate all six kharif legumes with high nodulation efficiency. Evaluation of these isolates for cellulase activity both on CMC agar plates and in liquid broth revealed that promiscuous rhizobial mutants showed highest cellulase activity both on CMC agar plates and in liquid broth as compared to other rhizobia. Molecular mechanism of rhizobial promiscuity was studied by amplifying and detecting relevant nodulation genes such as nodD, nodT, nodXandnolA in different rhizobia. PCR conditions were optimized to amplify a particular gene. All the 13 selected rhizobial isolates showed the presence of nodD and nodX genes, whereas amplification of nodT was observed in the isolates capable of nodulating 6, 5 and 4 different legumes. However, the expression of nolA gene was fairly good with single amplified PCR product in the rhizobia capable of nodulating 6, 5 and 4 different kharif legumes, amplification of nolA with single PCR amplified product of 672 bp was observed in isolates nodulating 6, 5 and 4 different while rest of the isolates showed poor expression of nolA gene with multiple bands 672 & 323bp. The mutant PKR188m85 performed better in terms of nodulation efficiency and plant growth parameters in pigeonpea, mungbean, clusterbean and mothbean whereas PKR177m30 performed better in cowpea and urdbean followed by PKR188m85 than their respective parent and reference strains under sterilized conditions.