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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Microbiology × Author: Anu × End date: 2005 × Start date: 2005 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Rhizosphere colonization of phosphate solubilizing bacteria and their effect on chickpea (Cicer arietinum L.) growth and nutrient uptake
    (CCSHAU, 2005) Anu; Kundu, B.S.
    Seventy six isolates of phosphate solubilizing bacteria from rhizosphere of chickpea were evaluated for solubilization of tricalcium phosphate (TCP) and Udaipur rock phosphate (URP). Solubilization efficiency (PSE) of various isolates varied from 6.0 to 118.3 per cent on solid medium. Sixty nine isolates showed < 50 per cent PSE and only 2 isolates showed PSE more than 100 per cent. P solubilization by all the isolates varied from 22.7 to 247.7 g/ml. Seven isolate showed >200 g/ml solubilization in liquid medium. Phosphate from rock phosphate was poorly solubilized which varied from 0.001 to 0.258 g/ml. No relationship between P solubilization and change in pH was observed. Only fifteen isolates have complementary antibiotic resistance with donor strain of E. coli. Morphological and biochemical characteristics of selected isolates based on P solubilization efficiency (18C, 42C, 43C and 76C) showed that they belong to genus Pseudomonas. The various transconjugants showed -galactosidase activity varying from 0.96 to 2.02 Miller’s Unit and showed blue colour on X-gal medium plates. The transconjugants (42CT and 76CT) were able to survive in chickpea rhizosphere under pot house conditions, which appeared as blue colonies on X-gal plates with a population of ~103/gram soil, upto 120 DAS. The PSB and their transconjugants showed an increase in dry shoot weight and nutrients uptake by chickpea. Dry matter yield of 61.81, 82.91 and 110.75 g/pot was recorded by the application of 30 kg SSP with PSB at 60, 90 and 120 DAS respectively. N and P uptake increased with the inoculation of PSB and their transconjugants. Significantly higher N uptake 31.54 and 66.31 per cent was recorded by the inoculation of SSP with 42C and SSP (60 kg/ha) at 90 and 120 DAS, respectively than control. At 90 and 120 DAS, maximum P uptake 92.56 and 114.0 per cent, respectively, was recorded by the inoculation of SSP with 42C than control.