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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Human Development and Family Studies × Author: Shanker, Shardul × End date: 2007 × Start date: 2007 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Evaluation of insecticidal activity of bacillus thuringiensis isolates S6 and cloning of cry1C gene in escherichia coli
    (CCSHAU, 2007) Shanker, Shardul; Kamla Chaudhary
    The increased public concerns of the potential adverse environmental effects of the extensive use of chemical insecticides, together with slow momentum of discovering second and third generation insecticides, has stimulated research worldover to screen native Bt population its bioefficacy against target insect pests. During the present investigation, studies were under taken to optimize conditions for production of biomass of Bt S6 using low cost substrates. Experiments were conducted to evaluate its bioefficacy against Helicoverpa armigera under field conditions. Attempts were also made to clone cry 1C of isolate S6 in E.coil. To reduce the cost of production medium, agro-byproducts can be used as the major ingredients for large scale production of B. thuringiensis biomass. Maximum biomass of Bt S6 (cfu) was produced when potato extract at a concentration of 13 % was used as carbon source. Although tryptone and peptone were found to be best nitrogen sources but because of high cost other low cost nitrogen sources were evaluated for biomass production. It was observed that cotton seed meal at 0.75 % level could be used for Bt S6 biomass production (7x109). The Bt S6 biomass produced in OPM was comparable to standard LB medium. B. thuringiensis isolate S6 at 1x109 cells/ml showed maximum insect mortality of 95-100 % against H .armigera under laboratory conditions after 96 h of treatment. However, under field conditions, insect mortality of about 76 % was observed in cotton when Bt culture was used at a concentration of 1x109 cells/ml. At lower cell concentration (1x108 cells/ml), insect mortality of about 73% was observed in cotton. The Optimum temperature for biomass production was found to be 350c. There was a significant decrease in the biomass production with increase or decrease in temp. The optimum pH of the medium for biomass production was found to be 6.00. To detect the presence of cry1C gene in Bt isolate S6, total genomic DNA was amplified by PCR using cry 1C gene specific primers. The PCR product of 1.5 kb size was amplified by using S6 DNA as template and cloned in E.coli. Four plasmid DNA samples out of 8 showed amplification with cry1C specific primers. However, the size of cry 1C gene is about 3.8 kb, suggesting that the cry 1C insert is a partial clone only.