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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2004 - 200912020 - 20231
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Subject: Genetics and Plant Breeding × Author: Meenakshi × Start date: 1997 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Graphical and conventional Line x Tester analyses for seed yield and attributes in fieldpea (Pisum sativum L.)
    (CCSHAU, Hisar, 2023-06) Meenakshi; Yadav, Rajesh
    In self-pollinated crop like fieldpea, recombinant breeding is the most desirable approach to combine various desirable attributes. In the present study, genetic variation, combining ability and heterosis were studied for days to flowering and maturity, primary and secondary branches per plant, nodes per plant, height of first pod, plant height, pods per plant, seeds per pod, 100-seed weight, biological and seed yield per plant and harvest index in fieldpea (Pisum sativum L.) using line x tester design involving ten lines, three testers and their 30 crosses and comparisons were made between the conventional and graphical approaches. The presence of adequate variability was revealed by significant differences among the parents and hybrids for almost all the traits studied. The meansquares due to parents vs crosses emerged significant for most of the traits revealing adequate amountof heterosis. Narrowdifferences between PCVand GCVof different traits indicated lesser influence of environment on the expression of these traits. Low heritability coupled with low genetic advance were observed for most of traits suggesting that these traits are genetically controlled by non-additive gene action. Among lines, HFP 715, RFP 2009-2 and GP02/1108 and among testers HFP 1711 exhibited good general combining ability and among the crosses Indira Matar 1 x HFP 1428, DDR23 x HFP 1711, GP 02/1108 x HFP 1428 expressed significant SCA for seed yield and its attributing traits according to conventional analysis. The lines and crosses exhibited superiorityin the present study can be exploited for isolating desirable transgressive segregants in advanced generations in future fieldpea improvement programmes. The σ2GCA/σ2SCA and the degree of dominance indicated predominant roleofdominancegeneaction.ThecrossesIndiraMatar1xHFP1711,AmanxHFP1711,GP02/1108 x HFP 1711, IPF 14-13 x HFP 1428 expressed significant desirable heterobeltiosis for seed yield andits attributing traits These may be utilised in crop improvement programme to obtain transgressive segregants in later generations. Graphical analysis results were almost same to those of traditional analysis with some variations. These methodologies provided easily interpretable output and it can be inferred that PCA biplot method can be used alone or together with GGE biplot method for evaluating parents and crosses in a line × tester design.
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    ThesisItemOpen Access
    Studies for genetic diversity for herbicide resistance in Phalaris minor Retz.
    (CCSHAU, 2004) Meenakshi; Saharan, R.P.
    Phalaris minor is most abundant grassy weed infesting wheat crop. Due to continuous use of herbicides the plants become resistant to these herbicides. The present study was conducted on 40 biotypes taken from different regions of Haryana state to standardize DNA extraction method and PCR amplification conditions and to analyze genetic diversity among various biotypes of P. minor. DNA was isolated using CTAB method with slight modifications. Biotype from Mundhal yielded highest amount of DNA (1404 ng/μl) and from Pirthala (lead treated) yielded lowest amount of DNA (110 ng/μl). Quality of DNA was tested on agarose gel electrophoresis and spectrophotometer. A single discrete band of high molecular weight showed that DNA was pure, free from contaminant, intact and of high quality. The ratio of absorbance ranged from 1.74 to 1.84. Clear and reproducible bands were generated by PCR amplification conditions of 50 ng genomic DNA, 1.6 mM MgCl2, 1 unit Taq DNA polymerase, 100 μM of each dNTP’s, 1 μl of 10X reaction buffer of Taq DNA polymerase 0.2 μM of primer and 40°C of annealing temperature. Of the 21 primers screened, 15 primers showed amplification while 6 primers did not show any amplification with any of the biotype of P. minor. All 15 primers were polymorphic showing 97 per cent polymorphism. In total, 102 bands were obtained of which 99 bands were polymorphic while 3 bands were monomorphic, generated by 15 primers. For the biotypes tested, 2 to 15 bands were obtained with an average of 4.85 per primer. Eight primers amplified 40 biotypes followed by one primer, which amplified 38 biotypes. All the biotypes were distinguishable with the combinations of polymorphic bands generated by various primers. Estimates of genetic similarity ranged from 0.489 to 0.885 indicating a high genetic variability among the biotypes. Based on the tree cluster analysis using NTSYS, the genetic variation among biotypes was high enough to group the biotypes in three clusters. First cluster comprising five biotypes (four susceptible and one resistant), second cluster has 18 biotypes (12 susceptible and six resistant) and third cluster has 17 biotypes (four susceptible and 13 resistant). The results indicate that RAPDs are efficient for grouping the resistant and susceptible biotypes i.e. most of resistant biotypes grouped into separate cluster showing that these are genetically different from susceptible biotypes. This information can be used further for identification of molecular marker for herbicide resistance gene(s) in Phalaris minor Retz.