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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2021 - 20222
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Subject: Genetics and Plant Breeding × Author: Kavita ×
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    ThesisItemOpen Access
    Transcriptomics studies in bread wheat under terminal heat stress
    (Chaudhary Charan Singh Haryana Agricultural University hisar, 2022-12) Kavita; Mukesh Kumar
    In the present investigation 50 wheat genotypes were sown with two replications in a randomized complete block design (RBD) at the experimental area of Department of Genetics and Plant Breeding, CCSHAU, Hisar, during Rabi seasons of 2018-2019 and 2019-2020 under normal and late sown conditions. Combined ANOVA showed that all traits had high level of genotypic variations among all the genotypes under both conditions. Highest heritability was shown by days to anthesis under both conditions while high GCV, PCV and genetic advance as percent mean were high for relative stress injury and chlorophyll stability index under normal and late sown conditions, respectively. Biochemical parameters and heat stress indices were used to screen out heat tolerant genotypes. Grain yield per plot exhibited positive and highly significant correlation with membrane stability index, chlorophyll stability index and all yield contributing traits under both conditions. Correlation among heat stress indices exhibited that grain yield per plot under both conditions is positively correlated with stress tolerance index, yield index, mean productivity, geometric mean and harmonic mean. Path analysis showed that harvest index and biological yield per plot had highly positive direct effects on grain yield per plot under both conditions. The principal component analysis for morpho-physiological traits under normal and late sown conditions and heat stress indices depicted that the first four, three and two components reflected more than 78%, 76% and 97% of the total variance, respectively. Cluster analysis grouped genotypes into 7 and 5 clusters, most divergent clusters were IV and VI, II and V under normal and late sown conditions, respectively. UPGMA cluster tree analysis divided genotypes into two clusters and also confirmed that sufficient variability is present among genotypes. Transcriptome analysis revealed that the differentially expressed genes (DEGs) under control and heat stress conditions were identified in both genotypes (WH-730 and WH-1184). These DEGs were utilised to set up a subsequent unigene assembly and GO analysis was done using unigenes to analyze functions of DEGs which classified into three main domains; biological process, cellular component and molecular function. KEGG ontology was used to visualize the physiological processes or to identify KEGG pathways that provide plants their ability to shield in adverse conditions of heat stress. From KEGG ontology it was reported that the gene encoded enzyme (peroxidase) of KEGG pathway was upregulated in WH-730 while downregulated in WH-1184.
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    ThesisItemOpen Access
    Exploring genetic variation for morphological and biochemical traits in pearl millet [Pennisetum glaucum (L.) R. Br.]
    (CCSHAU, Hisar, 2021-07) Kavita; Ramesh Kumar
    In present investigation, 50 pearl millet genotypes were evaluated in randomized block design with two replications at experimental field of Bajra section, CCS HAU, Hisar during kharif (Rainy) 2020. Analysis of variance showed that mean sum of squares due to genotypes were highly significant for all the characters studied, indicated the presence of sufficient genetic variability. Higher estimates of PCV than corresponding GCV, thereby, revealing that there is slight influence of environment on different traits. Estimates of GCV, PCV, heritability and genetic advance as a percentage of mean were high for the traits viz., grain Ca content, grain yield/plant, dry fodder yield/plant, number of productive tillers/plant, grain Mg content revealed importance of additive gene action and these traits could be exploited through simple recurrent selection procedure. Grain yield was significantly and positively correlated with dry fodder yield/plant, plant height, 1000-seed weight and panicle length suggests that direct selection of these characters will be effective for improvement of grain yield in future breeding programmes. Grain Ca and Mg content exhibited negative and non-significant correlation coefficient with grain yield/plant indicates that grain minerals can be improved without compromising on grain yield/plant. Higher magnitude of correlation coefficients between Ca and Mg content, suggest that both these traits can be simultaneously improved. The path coefficient analysis showed the maximum positive direct effect on grain yield by dry fodder yield/plant followed by plant height, panicle length, panicle diameter and grain Ca content. Therefore, in order to increase grain yield, effective selection can be accomplished for the characters having high direct effects. The germplasm lines viz., HMC-94-2, GP-69, GP-80, GP-70 and HMC-283 with high grain yield/plant (>30g) along with moderately high grain Ca (>160 mg/kg) and Mg (>1600 mg/kg) content were identified in present study. These high yielding germplasm lines with moderately high level of minerals confirm the possibility of simultaneous improvement of both type of characters in pearl millet.