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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2012 - 20162
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Subject: Genetics and Plant Breeding × Author: Bhusal, Nabin × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Characterization of elite forage sorghum {sorghum bicolor (L.) moench} genotypes
    (CCSHAU, 2012) Bhusal, Nabin; Pahuja, S.K.
    The objective of present investigation was to for characterize 20 genotypes of sorghum {Sorghum bicolor (L.) moench} on the basis of 33 morphological characters provided by PPV & FRA for DUS testing in sorghum. Results revealed that maximum variation was found among genotypes on the basis of glume colour in which genotypes G 46, HC 308, HJ 513 had green white, IS 3237, SSG 9, HC 171 had yellow white, SSG 59-3, COFS 29 had grayed purple, S 437-1, SGL-87, S 540-S, SSG (PSSG) had grayed yellow and remaining seven genotypes had grayed orange glume colour. Whereas, glume length varied from very short (three genotypes), short (six genotypes), medium (six genotypes), long (four genotypes) to very long genotype (COFS 29). On the basis of seedling anthocyanin colouration of coleoptiles and leaf sheath anthocyanin colouration of coleoptiles two groups were made i.e. yellow green (four genotypes) and grayed purple (sixteen genotypes). All genotypes showed simlar expression in both traits. In case of width of blade, genotype COFS 29 had Short (<4.1 cm), four genotypes had medium (4.1-6.0 cm), 14 genotypes had long (6.0-8.0 cm) and genotype IS 18551 had very long (>8 cm) width of blade. Genotypes were grouped into four groups viz., short (six genotypes), medium (eight genotypes), long (three genotypes) and very long (three genotypes) on the basis of panicle length without peduncle. Whereas, panicle density at maturity (ear head compactness) varied from very short (four genotypes), loose (three genotypes), semi loose (four genotypes), semi compact (eight genotypes) to compact in COFS 29. On the basis of panicle length of branches, genotypes were divided into four groups, two genotypes IS 3237 and HC 136 were short (<5.1 cm), ten genotypes were medium (5.1-10 cm), six genotypes were long (10.1-15 cm) and two genotypes COFS 29 and SSG 59-3 were very long (>15.1 cm). In case of neck of panicle visible above sheath, genotypes were divided into five groups viz., absent or very short (eight genotypes), short (four genotypes), medium (three genotypes), long (two genotypes) and very long (three genotypes). On the basis of grain texture of endosperm, genotypes were divided into four categories, genotypes SSG 9 and COFS 29 were vitreous, seven genotypes were ¾ vitreous, eight genotypes were ½ vitreous, and three genotypes G 46, SSG 5(22) and HJ 513 were ¾ farinaceous. Classification of genotypes on the basis of DUS traits provided identification of key characteristics of various genotypes. Broad sense heritability, GCV, PCV, correlations and path analysis were also carried out in these 20 genotypes.
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    ThesisItemOpen Access
    Genetic and molecular marker analysis for heat tolerance in wheat (Triticum aestivum L.em.Thell)
    (CCSHAU, 2016) Bhusal, Nabin; Sarial, A.K.
    Genetics of heat tolerance is quantitative and a complex phenomenon controlled by expression of numerous interacting quantitative trait loci (QTLs). To find out genomic regions associated with terminal heat tolerance a study entitled “Genetics and molecular marker analysis for heat tolerance in wheat (Triticum aestivum L. em. Thell)” was conducted. A mapping population of F8:9 of 251 recombinant inbred lines (RILs) of wheat derived from a cross between HD2808 (tolerant) x HUW510 (susceptible) was genotyped and phenotyped along with their parents under normal and stress (terminal heat) conditions during 2013-14 and 2014-15 Rabi season in a RBD. Data were recorded for various morpho-physiological traits including grain yield, 1000-grain weight, grain filling duration, grain filling rate, grain numbers/main spike, grain weight/main spike and harvest index. HSI of each trait was used to find out the effect of heat stress. Analysis of variance revealed significant differences for all traits studied over sowing conditions and years. DNA was isolated following CTAB method for genotyping. Total 380 SSR markers representing approximately 18 on each chromosome were used to detect parental polymorphism. Parental polymorphism was obtained 21.5%. Linkage map was constructed using 52 polymorphic primers representing 8 linkage groups. Composite interval mapping over conditions and years identified 86 QTLs with maximum 36 QTLs under timely sown (non stress), 31 QTLs under late sown (heat stress) and 19 for HSI of traits. Favorable alleles for QTLs associated with heat tolerance were associated with HD2808. QTLs were detected for all morpho-physiological traits studied except for effective number of tillers. Maximum QTLs were detected for days to heading (9). Grain yield related trait for which parents genetically differed under heat stress like, grain weight/main spike and grain number/spike had (8) QTLs each while, 1000-grain weight (7). Of these, three QTLs were found consistent and stable over the conditions and years. They were located between gwm448 and wmc296 markers, with a marker gwm122 embedded onto QTL region. 1000-grain weight recorded maximum (30.1%) phenotypic variance, was flanked with markers gwm448 at 6.35 cM and wmc296 at 8.6 cM distance followed by grain number (201.1%) at 5.7 and 9.7 cM and grain weight (19.8%) flanked at 4.5 and 7.4 cM. These markers could be utilized for marker assisted selection for screening germplasm and breeding for heat tolerant cultivars.