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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Genetics × Author: Chauhan, Sapna × End date: 2009 × Type: Thesis ×
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    ThesisItemOpen Access
    Rhizobacterial strains as biocontrol agents against various soil pathogens
    (CCSHAU, 2008) Chauhan, Sapna; Vasudeva, Manjula
    A total of fifty three isolates/mutant were screened for their antagonistic potential against three selected fungal soil pathogens; Rhizoctonia solani cotton, R. solani rice and Fusarium oxysporum. Out of these 10, 6 and 12 isolates/mutant of Azotobacter chroococcum were selected against R. solani cotton, R. solani rice and Fusaraium oxysporum tomato respectively. 19.61% showed zone of inhibition against R. solani cotton, 11.76% against R. solani rice and 23.53% against Fusarium oxysporum tomato respectively. Further quantitative studies were made and these zones of inhibition were measured. In R. solani cotton, the zone was maximum (2276.5 mm2) with AH2 and minimum (141.3 mm2) with AM1. Similarly, in R. solani rice, the zone of inhibition was maximum (1243.44 mm2) with AVK42 and minimum (120.5 mm2) with AR4 and in Fusarium oxysporum, maximum (1004.8 mm2) with HT54 ii and minimum (164.05 mm2) with 103. Further, various studies were done to isolate, evaluate and characterize the antimicrobial substance responsible for this activity. The antimicrobial substance responsible for the antagonistic activity was found to be extracellularly produced and it was not thermostable. TLC assays were done to confirm the production of antifungal and antibiotic compounds taking Pseudomonas as standard. The invitro results were further checked in pothouse conditions on three crops, cotton, guar and tomato respectively. Increase in percent germination was observed. Maximum percentage of disease index (80.36 % and 93.75% on 60th day) was observed with R. solani cotton and R. solani rice check respectively while minimum disease index was seen in case of 103DS (16.67 %) in R. solani cotton and (2.5%) with 103DB in R. solani rice in cotton crop. Similarly in guar crop, maximum percentage of disease index (95.24% and 85.93% on 60th day) was observed with R. solani cotton and R. solani rice check respectively while minimum disease index was seen in case of AH2 (2.5%) in R. solani cotton and (13.29%) with AR4 in R. solani rice. Further in case of tomato, maximum disease index (85 % on 60th day) was observed in control Fusarium oxysporum check and was negligible in strains HT57, AP4, AVK42, AVK51, 103, AH2 and 103DS. The disease was completely controlled by these selected isolates/mutants.