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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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20192
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Subject: Biotechnology and molecular Biology × End date: 2019 × Start date: 2019 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Development of micro-RNA and EST-SSR markers for diversity analysis in clusterbean [Cyamopsis tetragonoloba (L.) Taub]
    (CCSHAU, Hisar, 2019-09) Chaudhary, Vrantika; Yadav, Neelam R
    Cluster bean [Cyamopsis tetragonoloba (L.) Taub] is an important industrial crop due to the presence of soluble dietary fibre, high protein content and high value gum. MicroRNA are tiny non-coding small RNAs master regulators that play important role in multiple biological processes by degrading targeted mRNAs or repressing mRNA translation. In silico computational analysis based on Cluster bean ESTs led to identification of 57 miRNA families along with 37 targets. The length of most of the mature miRNAs was found to be 21nt long and average MFE value of 25.4 (-kcal/mol). The gene ontology study revealed the genes encoding transcription factors or enzymes involved in the regulation of development, growth, metabolism, physiological processes and stress response. Identification of transcription factors and miRNA resulted into model prediction. Most abundant miRNA families predicted in this study were miR156, miR172 and miR2606. The miRNA related to gum metabolism were also identified i.e. miR5658, miR1533 and miR414. A total of 40 unigene SSRs were screened among hundred germplasm lines including selected cultivars. The molecular analysis divided the 100 cluster bean genotypes into two major clusters at similarity coefficient of 0.49. Genic SSRs related to galactomannan metabolism were also used for diversity analysis of low and high gum clusterbean genotypes. A new class of functional and regulatory markers i.e., miRNA-SSRs have been identified in Cluster bean with mononucleotides repeats in highest proportion (76%) followed by di- (10%), tri- (10%) and tetranucleotides (4%) in the pre-miRNA sequences. Cross transferability of Medicago truncatula miRNA SSRs showed 34% cross transferability among Cluster bean genotypes. A key enzyme in galactomannan metabolism, galactosyltransferase was used for structure prediction using in silico approaches. In silico molecular dynamics revealed its stability and structure was dominated by random coil and α-helix followed by β sheet region. Cluster bean galactosyltransferase sequence showed highest similarity with fenugreek and least similarity with Medicago truncatula
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    ThesisItemOpen Access
    Development of transgenic pigeon pea (Cajanus cajan L.) plants containing Lectine receptor like kinase gene for improving abiotic stress tolerance
    (CCSHAU, Hisar, 2019-05) Pratibha; Kharb, Pushpa
    Pigeon pea (Cajnus cajan L.) is a nutrient rich, sixth most important and second most important legume pulse crop of the world and India respectively after chickpea. Production and productivity of pigeon pea is affected by various abiotic stresses but salinity is the most deleterious stress which causes considerable loss in crop’s productivity. In the present study, transgenic pigeon pea plants (cv.. Manak) carrying OsLecRLK gene, were developed for salinity tolerance using Agrobacterium tumefaciens strain LBA4404 with pCAMBIA1300- OsLecRLK gene (Kharb et al., 2018 Patent Application No. 201811012099). Putative transformants were screened through PCR amplification using gene specific primers and out of 86 plants 16 plants were found positive giving a transformation efficiency of 18.6%. Six higher yielding lines were selected and checked for stable transgene integration. Southern hybridization and Real-time PCR analysis was done to find out the copy no. of transgene in selected transgenic lines. Seeds from T0 pigeon pea plants were sown to raise T1 generation and a total of 52 plants (out of 120) showed the presence of OsLecRLK gene. Physiobiochemical analysis was done to assess the efficacy of transgene via subjecting wild type and selected T1 transgenic plants to 75 mM salt stress. The results showed that transgenic line performed better in terms of maintaining higher chlorophyll content, relative water content, proline content, total soluble sugar content, peroxidase and catalase activity in comparison to the wild type plants. Moreover, MDA content and membrane injury index were significantly reduced in transgenic lines when compared to the wild type plants indicating that the transgenic lines were less affected by salt stress. Among all transgenic lines, L-19 performed well with respect to all the parameters studied and can be taken further for the development of transgenic pigeon pea plants for salt stress tolerance.