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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Biotechnology and molecular Biology × Author: Mehla, Sheetal × Start date: 2011 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Characterization and transcriptome analysis of transgenic pigeon pea (Cajanus cajan (L.) Millsp.) plants with LecRLKgene for salt tolerance
    (CCSHAU, Hisar, 2023-01) Mehla, Sheetal; Upendra Kumar
    Receptor kinases are one of the most potent cell signaling molecules that work against different kinds of stresses, however, very few studies are there on the characterization of the Lectin Receptor-like Kinase gene in plants for regulating salt stress. Biochemical analysis of increased concentrations of antioxidant enzymes like catalase, peroxidase and osmolytes like proline and total soluble sugar deciphered the role of OsLecRLK in maintaining the osmotic balance, ion homeostasis, and keeping a check on ROS production. Also, we observed a significant decrease in membrane injury index and an increase in the physiological parameters like Relative water content, chlorophyll content and photosynthetic rate confirming the role of OsLecRLK in providing salt tolerance in transgenic pigeon pea plants. Analysis of phenological and yield attributes confirmed that the mechanism governed by this gene is ultimately maintaining the vigor of the pigeon pea plants. A potential increase of 23-26% in the harvest index of transgenic lines conferred the completion of the pigeon pea life cycle in a normal pattern with very minimal effects of salt stress. OsLecRLK expressing pigeon pea lines were found to perform much better than wild types starting from vegetative till the completion of the reproductive phase. Through comparative transcriptome analysis, the intrinsic mechanism responsible for providing salt tolerance in transgenic pigeon pea plants with the OsLecRLK gene was unveiled. The relative expression analysis of histone deacetylase1, acyl CoA, ascorbate peroxidase, peroxidase, glutathione reductase and catalase genes were found higher and the expression of indole acetic acid synthetase, calmodulin and ketoacyl genes were found much lower in transgenic in comparison to wildtype pigeon pea plants under salt stress.