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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Biotechnology and Molecular Biology × Author: Saha, Abhigyan × Start date: 1980 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Identification of gene for ADP-Glucose pyrophosphorylase from developing grains of wheat
    (CCSHAU, 2006) Saha, Abhigyan; Sikka, Virendera K.
    A step towards isolation of gene(s) has been undertaken in the proposed study. In order to achieve the target of AGPase cDNA gene library preparation, first of all total RNA was isolated from developing grains (14 DAA), of wheat varieties C306 and WH711. Modified RNA extraction protocol was employed, which gave fairly high quality RNA. Good quality mRNA was fractionated from total RNA using poly AT tract magnetic beads mRNA isolation system (Promega). Complementary DNA (cDNA) library was generated from the mRNA of C306 and WH711 using Lambda zap cDNA kit (Stratagene). Plaque lifts were hybridized with rice AGPase gene probe to identify clones containing wheat AGPase gene. However, exact clone could not be isolated. AGPase enzyme assay was carried out in wheat varieties C306, WH711, WH147M, SG70, MLU-2, at 14DAA and 28DAA. The enzyme activity showed an association with rate of starch accumulation and grain size and yield.
  • Thumbnail Image
    ThesisItemOpen Access
    Identification of gene for ADP-Glucose pyrophosphorylase from developing grains of wheat
    (CCSHAU, 2006) Saha, Abhigyan; Sikka, Virendera K.
    A step towards isolation of gene(s) has been undertaken in the proposed study. In order to achieve the target of AGPase cDNA gene library preparation, first of all total RNA was isolated from developing grains (14 DAA), of wheat varieties C306 and WH711. Modified RNA extraction protocol was employed, which gave fairly high quality RNA. Good quality mRNA was fractionated from total RNA using poly AT tract magnetic beads mRNA isolation system (Promega). Complementary DNA (cDNA) library was generated from the mRNA of C306 and WH711 using Lambda zap cDNA kit (Stratagene). Plaque lifts were hybridized with rice AGPase gene probe to identify clones containing wheat AGPase gene. However, exact clone could not be isolated. AGPase enzyme assay was carried out in wheat varieties C306, WH711, WH147M, SG70, MLU-2, at 14DAA and 28DAA. The enzyme activity showed an association with rate of starch accumulation and grain size and yield.