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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2009 - 200912010 - 20161
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Subject: Biotechnology and Molecular Biology × Author: Pardeep Kumar × End date: 2020 ×
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    ThesisItemOpen Access
    Identification of QTL(s) for terminal drought tolerance in chickpea (Cicer arietinum L.)
    (CCSHAU, 2016) Pardeep Kumar; Kharb, Pushpa
    Drought stress, particularly at the end of the growing season i.e. terminal drought stress is a major constrain production and yield stability in chickpea (Cicer arietinum L.). Identification of genomic regions contributing to drought stress tolerance can help to develop better chickpea varieties through pyramiding of favorable alleles into adapted genotypes by molecular marker-assisted breeding. In the present investigation, experiments were conducted to evaluate two F2 populations derived from crosses between (HC-1 and ICC 4958) and (HC-1 and RSG 931), for genotyping by SSR markers and F2:3 populations for phenotyping of phenological, growth-related; yield and yield-related; and physiological traits. F2:3 progeny lines of cross HC-1 × ICC 4958 displayed large variation for phenological, growth-related; yield and yield-related; and physiological traits. Root depth and root dry weight was found to be positively correlated with seed yield. Similarly relative water content, osmotic potential and photochemical efficiency of photosystem II was positively correlated with seed yield while water potential, relative stress injury and CTD was positively correlated with seed yield. A DNA fingerprint database of 128 F2 progeny lines of cross, HC-1× ICC 4958 was prepared using 27 polymorphic SSR markers. F2:3 design was used to map the QTLs for drought stress tolerance using WinQTL cartographer 2.5 and a total of 16 novel QTLs were identified for growth-related traits (3); yield and yield-related traits (6); and physiological traits (7) on linkage group 7. Out of 16 identified QTLs, five were major QTLs, one each for root depth, 100 seeds weight, biological yield, osmotic potential of leaf and relative stress injury of leaf, which were contributing more than 15% in phenotypic variance. In another cross, F2:3 progeny lines of cross HC-1 × RSG 931 displayed variation for phenological, growth-related; yield and yield-related; and physiological traits. Root length was found to be negatively correlated with yield in 18 F2:3 progeny lines of cross HC-1 × RSG 931. DNA fingerprint database of 18 F2 progeny lines of cross HC-1 × RSG 931 was prepared using 25 polymorphic SSR markers, NTSYS-pc analysis and two-dimensional PCA scaling exhibited that 18 F2 progeny lines were interspersed between the two parental chickpea genotypes. In conclusion, after validation, QTLs identified in the present study can be used to introgress in elite chickpea genotypes by marker assisted selection to develop drought tolerant varieties.
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    ThesisItemOpen Access
    Expression of myb gene in brassica tournefortii L.under drought stress
    (CCSHAU, 2009) Pardeep Kumar; Yadav, Neelam R.
    Brassica tournefortii, a highly drought tolerant Brassica species was used to study myb gene expression under drought stress. Seeds of Brassica tournefortii were grown on MS medium. Then 14 days seedlings were uprooted and given drought stress by subjecting them to air drying, PEG (-2 to -8 bar) and mannitol (100 mM to 400 mM) treatments. Total RNA isolation from stressed seedlings was carried out using Trizol reagent yielding 17.52-36.8 Fg/ml of total RNA. One- step RT-PCR was carried out using total RNA as template with BjMyb-1 primer designed from conserved domain of AtMyb2 and its homologous. BjActin primers were used in RT-PCR which served as control, as actin gene is constitutively expressed in all tissues. Exposure to drought stress for 15 minutes and 30 minutes (air drying) gave no amplification showing air drying upto 30 minutes does not induce any myb expression. An amplified product of 250 bp was obtained on exposure to drought stress for 60 minutes with air drying, PEG (-2 to -8 bar) and mannitol (100 mM to 400 mM) treatments. The transcript level was found similar in all treatments irrespective of drought treatments. cDNA was eluted out from the gel and purified cDNA was transformed using pDrive cloning vector (Quigen) in XL-blue strain of E.coli using blue- white selection. Transformed clones were characterized by plasmid DNA isolation, PCR amplification of plasmid DNA with gene specific primers. Plasmid DNA from transformed clones showed higher molecular weight than untransformed plasmid DNA on agarose gel electrophoresis confirmed the insertion of DNA fragment into the plasmid. PCR amplification of plasmid DNA also confirmed the successful cloning.