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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2005 - 200912010 - 20112
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Subject: Biotechnology and Molecular Biology × Author: Bajaj, Anshu × Start date: 2000 × Type: Thesis ×
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    ThesisItemOpen Access
    Molecular mapping of bacterial blight resistance gene, drought tolerant QTL(s) and genetic diversity analysis in clusterbean {Cyamopsis tetragonoloba(L) Taub}
    (CCSHAU, 2011) Bajaj, Anshu; Boora, K.S.
    The present investigation was carried out to screen genetic diversity using RAPD markers and to identify and map the QTL for resistance to bacterial blight and drought tolerance in clusterbean .The plant material was procured from Forage section . For bacterial blight, the resistant (HG 75) & susceptible(PNB) genotypes were selected & crossed to generate F3 population. The F3 population was used to map QTLs. For drought tolerance studies genotypes HG 563 (tolerant) & PNB (susceptible) were used to generate the mapping population (F 2:3).The genetic diversity studies were carried out in 48 genotypes of clusterbean using 130 RAPD primers and 97 primers produced amplification of 705 alleles ;out of which 644 were polymorphic. The similarity ranged from 0.11 to 0.97.The dendrogram grouped the genotypes into two major clusters at a similarity coefficient of 0.68. A variety FS277 was out grouped which is a forage variety. The genotype GP96 and F5277 were found to be most diverse (89%).The QTLs were identified for bacterial blight resistance using parental genotypes (HG75 & PNB) & their F3 population. The F 3 population was screened artificially for disease resistance along with the parent genotypes. Polymorphic primers were used to screen the F3 population & the major QTLs were identified for disease resistance using parental genotypes, HG75, PNB and their F 3 population. A total of 83 RAPD primers were used to screen the parental genotypes out of which nine primers were polymorphic. These primers were used to screen the F 3 population and the QTLs found were L19 , D1, AB7 and QLTY 3. Parental genotypes HG563 and PNB and their F2-3 genotypes were screened at field and net house for resistance to drought. Out of fifty genotypes, 12 were highly tolerant, 23 were moderately tolerant and 15 were susceptible to drought. The parents were screened with eighty seven primers out of which, sixteen were found polymorphic and nine QTLs were found linked to drought tolerance. Loci OPQ 20,OPD10, OPD14,OPQ 12,OPAC 8 and OPF 9 were major contributing QTLs and OPAC2,OPL3 and OPF17 are minor QTLs The diverse lines may be used for breeding programs to improve the production of agronomically superior genotypes of guar. The major QTLs for bacterial blight and drought tolerance may be used for introgression into agronomically superior genotypes of guar.
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    ThesisItemOpen Access
    Molecular Mapping Of Bacterial Blight Resistance Gene, Drought Tolerant QTL (S) And Genetic Diversity Analysis In Clusterbean {Cyamopis Tetragonoloba (L) Taub}
    (Chaudhary Charan Singh Haryana Agricultural University, Department Of Molecular Niology And Biotechnology : Hisar, 2011) Bajaj, Anshu; Boora, K. S.
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    ThesisItemOpen Access
    Genetic improvement of Saccharomyces spp. for ethanol production from starch
    (CCSHAU, 2005) Bajaj, Anshu; Chaudhary, Kamla
    A study was undertaken to improve ethanol production from starch by yeast Saccharomyces spp. A total of 10 isolates of yeast were isolated. After secondary screening in submerged culture conditions six isolates were selected for further investigation. Based on Maximum (6.4U) amylase and ethanol (8.8g/l) production, the Saccharomyces spp. B6 was selected and mutagenized using UV rays and nitrous acid. The Saccharomyces spp. mutant B6U3 produced maximum amylase (8.9U) & ethanol 16.4g/l (in starch) and 26.8g/l (in dextrose) among various mutants and was selected for further studies. The optimization of culture conditions showed that Saccharomyces spp. B6U3 produced increased level of amylase as compared to parent strain. Ethanol production was found to increase with increase in the level of starch. At high concentration of starch the ethanol production was decreased. Starch at a concentration of 10% was found be the best for production of ethanol by Saccharomyces spp. B6U3. Peptone was found to be best nitrogen source for production of ethanol by Saccharomyces Spp. B6U3 producing a maximum 33.4 g/l of ethanol in 48h. In media containing ammonium sulphate, ammonium nitrate casein & urea less amount of ethanol was produced. Peptone was found to the best nitrogen source at 2% level and 34.4g/l of ethanol was produced in 48h of fermentation. Increase in the conc. of peptone led to decreased ethanol production. On increasing the inoculum size upto 20% the ethanol production also increased from 5.6g/l to 32.8g/l after 48h of fermentation. A little variation was found on using 20% of starch grown and dextrose grown inoculum. There was a slight increase in the ethanol production on using dextrose grown inoculum (34.8g/l) than starch grown (33.2g/l) after 48h of fermentation. Ethanol production was found to be maximum after 48h of fermentation. Out of different starch sources used Soluble starch, Sorghum, spoiled wheat, Potato and Cassava, soluble starch was found to be the best substrate for production of ethanol by Sacchromyces spp. B6U3.