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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2000 - 20062
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Subject: Biotechnology × End date: 2006 × Start date: 2000 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Molecular analysis of anthracnose resistance gene(s) in sorghum [Sorghum bicolor (L.) Moench)
    (College of Basic Sciences and Humanities Chaudhary Charan Singh Haryana Agricultural University Hisar, 2000) Pandey, Sunita; Boora, K. S
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    ThesisItemOpen Access
    Agrobacterium mediated transformation of indica rice variety HKR46 with amaranthus seed albumin gene, AmA1
    (CCSHAU, 2006) Parveen; Kharb, Pushpa
    Experiments were conducted to transfer the Amarantus seed albumin gene, AmA1, driven by CaMV35S promoter in indica rice variety HKR46 by Agrobacterium-mediated transformation. The japonica rice variety TNG67 was also used as control for transformation. During the present investigation, 15-18 days old calli initiated from mature seed scutella and immature embryos on modified MS medium were used. The calli were co-cultivated for 10 minutes in liquid co-cultivation medium with Agrobacterium strain EHA105 (pSB8) and then transferred onto the solidified co-cultivation medium for two days. After two days the co-cultivated calli were transferred onto selection medium containing kanamycin/paromomycin (50 mg/l) for 2-3 cycles of selection of 15 days each. A total of 13/1717 and 103/278 calli of HKR46 and TNG67 respectively showed sustained proliferation in the selection medium. The putatively transformed calli were then transferred onto regeneration medium. No shoot regeneration was obtained in case of indica rice variety HKR46. However, eight of the putatively transformed calli of TNG67 regenerated shoots; but all of these shoots were albino. The study shows an urgent need of developing an efficient protocol for high frequency green plant regeneration/genetic transformation in indica rice varieties.