Thumbnail Image

Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

Browse

20231
Reset filters
Subject: Bioinformatics × Author: Gahalyan, Dushyant × Start date: 2008 × Type: Thesis ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    In-silico identification of phytochemicals as potential agents to inhibit the growth of pests
    (CCSHAU, Hisar, 2023-05) Gahalyan, Dushyant; Panwar, Anil
    Compounds known as phytochemicals have a specific biological effect against pests. Insect molting and metamorphosis are controlled by ecdysteroids and the juvenile hormone. The natural hormone responsible for molting in the majority of insects, 20-hydroxyecdysone (20E), binds to and activates the ecdysone receptor (EcR), a heterodimer made up of two nuclear receptor proteins. EcR is an attractive target for safer and more targeted pesticides since compounds that act on it exclusively harm insects and other arthropods. Farmers mainly rely on quick pest management strategies to defend crops from pest attacks, primarily synthetic insecticides. Overuse and abuse of synthetic pesticides can have harmful effects on people, the environment, and non-target animals, which reduces biodiversity. Some toxic pesticides that could be extremely detrimental to mammals or the environment are aldicarb, malathion, parathion, etc. Many crop pests and human ailments have been successfully controlled by plants that produce bioactive compounds. High-throughput screening technologies have been created to effectively look for novel chemicals that interfere with the insect endocrine system. These methods will support the creation of ecdysone agonists that are particular for insect orders like Lepidoptera, Diptera, or Coleoptera's EcRs. The two receptors that were complexed with ligands were employed in this work to estimate binding affinity and predict binding mode. The potential orientation of a ligand with respect to a protein target can be determined extremely effectively using molecular docking. In this study, 93 phytochemical agents are docked against the main receptor of Heliothis virescens, and Bemisia tabaci. Five-five candidates are selected according to their high binding affinity for each insect, i.e. Helitohis Virescens (1R1K) and Bemisia tabaci (1Z5X). It is observed that for 1Z5X (Uscharin, Meliantriol, Campesterol, Daturaolone, and Epoxyazadiradione, which had binding affinities of -13.61, -13.28, -13.26, -12.29 and -12.24, respectively) were selected and for 1R1K (Campesterol, Azadirone, Uzarigenin, Meliantriol, and Pyrethrin-2 which had binding affinities of -13.12, -11.48, -9.94, -9.84 and -9.37, respectively) were selected. According to molecular dynamics simulation (20ns) investigations of the selected compounds, the top five docked 1R1K and 1Z5X compounds appear to have strong stability, flexibility, and binding affinity for ecdysone receptors.