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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Bioinformatics × Author: Bishnoi, Stela × Start date: 1981 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    In-silico Prediction of microRNAs and their targets in Wheat (Triticum aestivum L.)
    (CCSHAU, 2019) Bishnoi, Stela; Sudhir Kumar
    Wheat (Triticum aestivum L.), a staple crop which constitutes major part of human diet, belongs to family Poaceae or Gramineae family. As a major constituent of human diet it is therefore one of the most widely cultivated food grain in not only India but the world as well. In India it is the grain with second largest consumption after rice. The miRNAs play a major biological role by modifying the expression levels of a diverse repertoire of genes in a sequence in a dependent manner which is attained at either transcriptional or post-transcriptional level. The Nucleic acids sequences of the 21- chromosomes of Triticum aestivum were downloaded from the International Wheat Genome Sequences Consortium. After downloading wheat genome sequence from IWGSC, the DNA sequence is transcribed into RNA sequence and later split through split.pl which gave an output of 138 fragments. Then after finding out palindromes of fragments using palindrome finding perl script (palindrome.pl). The fragments with at most four mismatches were stored in a file. Secondary structure was predicted using RNAfold server. The sequences are sorted out which have MFE of secondary structure less than -40kcal, and considered as putative miRNAs. From BLAST there identified 699 new miRNAs having 111175 targets. These target genes have regulatory function in certain biological processes.