Thumbnail Image

Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

Browse

20171
Reset filters
Subject: Biochemistry × Author: Naresh Kumar × Start date: 2003 × Thesis Type: Ph.D ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    Biochemical changes in tomato (Solanum lycopersicum L.) under the influence of salicylic acid
    (CCSHAU, 2017) Naresh Kumar; Singal, H.R.
    The present investigation was aimed to study the effect of exogenous application of salicylic acid on two tomato varieties Hisar Arun (Short shelf life) and BSS-488 (Long shelf life). Tomato fruits of both varieties were harvested at mature green and turning stage and treated with the salicylic acid at different concentrations (0.5 mM, 0.75 mM, 1.0 mM, 1.25 mM and 1.5 mM). The fruit sample was analysed for various morpho-physiological and biochemical parameters at every 3rd day after salicylic acid treatment. The molecular parameters were analysed only at optimized concentration of salicylic acid (i.e. 0.75 mM) afterwards. The morpho-physiological and biochemical parameters like PLW, TSS, color change, lycopene, β-carotene, PG and cellulase increased progressively while the parameters like cellulose, hemicellulose, lignin and pectin decreased gradually throughout the storage period which leads to the ripening of tomato. The exogenous application of salicylic acid mainly at concentrations 0.75 mM improved the shelf life of tomato by delaying the change in the above mentioned morpho-physiological and biochemical parameters. Moreover, during ripening the parameters such as total sugars, reducing sugars, non-reducing sugars, ascorbic acid, ethylene, PPO and PME activity increased initially and declined thereafter with advanced ripening in control as well as in all treated fruits. However, the treated fruits at SA concentration 0.75 and 1.0 mM expressed the delay in the initial increase in above parameters. Similarly, the expression of ripening related genes (ACS and ACO) of tomatoes of both varieties at both maturation stages increased initially and decreased thereafter which is delayed by the exogenous application of salicylic acid. In addition, the quantitative analysis (via PD Quest) of 2-DE gels of tomato proteins demonstrated the overlapping gel coordinates between control and treated fruits. But the treated fruits expressed the differentially expressed protein spots that showed >1.5 fold expression change as compared to control. So it can be concluded that the treatment at 0.75 mM concentration of salicylic acid was most effective in enhancement of shelf life of tomato fruits.