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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Aquaculture × Start date: 2011 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Optimum utilization of Delonix regia leaf meal in the diet of Labeo rohita (Hamilton, 1822)
    (CCSHAU, Hisar, 2023-05) Lowanshi, Ashutosh; Gulati, Rachna
    During present investigation, groundnut oil cake and deoiled rice bran were replaced by Delonix regia leaf meal under varying proportions to evaluate their effect on survival, growth, hematological parameters and enzymatic activity of Labeo rohita. On dry matter basis, groundnut oil cake, deoiled rice bran and D. regia leaves contained 42, 13 and 22.12 percent crude protein, respectively. Inclusion of D. regia leaf meal at 60 percent as deoiled rice bran replacement significantly increased the average weight (3.42g), average daily weight gain (0.22g), total weight (123.28g), total weight gain (34.26g), biomass (123.29g), percent increase in length (12.56%) of L. rohita as compared to control (2.61g, 0.17g, 89.07g, 26.13g, 89.07g, 10.26%, respectively). Percent increase in weight, specific growth rate, length of L. rohita was significantly higher at (62.21%, 1.25%,7.11cm) at 100 percent replacement of deoiled rice bran with D. regia leaf meal than at 0 (50.91%,1.14%, 6.06cm) percent replacement diet. The mean values of the average weight, total weight, weight gain of L. rohita were significantly higher in treatment fed with 20 percent inclusion of D. regia as replacement of groundnut oil cake as compared to other treatments and control with 0 percent inclusion of D. regia, respectively. Biomass and average daily weight gain of L. rohita were recorded highest in treatment fed with 50 percent inclusion of D. regia (114.25g, 0.30g) and lowest in the control group (99.42g, 0.16g). D. regia leaf meal diets did not show any adverse effect as 100 percent survival of L. rohita was recorded in all the treatments. Minimum FCR (1.56) was observed in D. regia leaf meal (30%) as replacement of groundnut -oil cake diet and maximum (1.94) in control diet. The protein efficiency ratio (PER) of the diet fed to L. rohita was found to be statistically higher (0.64) in 30 and 20 percent (0.60) D. regia leaf meal as replacement of groundnut -oil cake diet. Replacement of deoiled rice bran with 60 percent D. regia leaf meal gave better results in terms of percent increase in length (12.56%) and specific growth rate (1.25%) as compared to 30 percent D. regia leaf meal replacement of groundnut oil cake (4.47%, 1.08%, respectively). Feed conversion ratio (1.47) and protein efficiency ratio (0.68) were also better in 30 percent D. regia leaf meal replacement of deoiled rice bran than at 30 percent D. regia leaf meal replacement of groundnut oil cake (1.56, 0.64, respectively). Apparent digestibility of D. regia leaf meal diets for 40 and 100 percent replacement of groundnut oil cake and deoiled rice bran was 77.5 and 77.97 percent, respectively. The pH, dissolved oxygen, and temperature remained in the range of 7.03 to 7.40, 6.02 to 6.13 ppm, and 24.66 to 25.56oC during the course of study showing no effect of D. regia diets.Free ammonia in different treatments remained under permissible level (0.05 to 0.09 ppm). Significantly higher total dissolved solids (79.50 to 91.52 ppm) were recorded in D. regia inclusion diets as compared to control diet (75.07 ppm). Haemoglobin and Haematocrit value of L. rohita was lowest in control diet (8.28g/dl, 22.8%) which significantly increased to 9.24 g/dl and 27.8 percent in 50 percent D. regia inclusion as replacement of groundnut oil cake. No significant effect of treatment diets was recorded on red blood cells and white blood cells. Red blood cell and white blood cell counts remained within the range of 2.39 to 3.20 × 106 μl and 17.49 to 17.52×103/μl, respectively. Effect of D. regia leaf meal on enzyme activity of L. rohita showed significantly lower Lipase activity (1.41 μM/mg protein/min) at 50 percent D. regia inclusion. D. regia leaf meal treatment diets did not influence the amylase and protease activity significantly. Amylase activity ranged between 18 to 25.50 μM/mg protein/min whereas, protease activity ranged from 24.21 to 28.59 Fluorescence change/unit. The anti-nutritional factors viz., total phenolic content, total oxalate content, tannin content, phytic acid and total alkaloids in the ethanolic extract of Delonix regia leaf were 31.24 mg gallic equivalents (GAE)/g sample in dry weight, 1.28mg/l, 17.80mg/l, 2.43mg/100g and 26.97 mg/100g, respectively.