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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Evaluation of therapeutic efficacy of Amaranthus hypochondriacus seed extract in fipronil intoxicated rats with special reference to In Silico prediction of squalene-CYP3A1 gene interaction
    (G.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145, 2022-08) Verma, Manish Kumar; Singh, S.P.
    This study was designed to evaluate the protective efficacy of Amaranthus hypochondriacus seed extract (HSEAH) following its oral administration @ 100 mg/kg b. wt. alone and simultaneously with fipronil @ 24.25 mg/kg b. wt. orally for 90 days in rats by measuring haematobiochemical, oxidative, reproductive, histopathological examination, impact on gene expression of CYP3A1 and residue analysis parameters. Hydroethanolic extract of the seed of Amaranthus hypochondriacus was prepared for phytochemical analysis and in vitro evaluation of antioxidant potential was undertaken in-silico prediction of squalene-CYP3A1 gene interaction. Phytochemical analysis revealed the presence of various phytochemical like alkaloid, flavonoids, carbohydrates, proteins, glycosides, oils, phenolic compounds, terpenoids, saponins and tannins whereas the coumarins, resins and steroids were absent. Mineral estimation reveled presence of calcium, iron magnesium, zinc, and copper was present in higher concentration whereas lead, cobalt and nickel in trace amount. In-silico study of cross linking comparison between squalene and fipronil-CYP3A1 gene interaction was found to have high binding affinities for squalene than fipronil. In vitro antioxidant activity assessed by measuring NO radical scavenging, DPPH radical scavenging, reducing power activity, and metal chelating of Fe+2 activities which revealed the excellent antioxidant potential of extract. For evaluation of protective efficacy of HSEAH in fipronil intoxicated rats, twenty four male Wistar rats weighing 90-100 gm of 4-6 weeks of age were divided equally and randomly into four groups. Group I served as control, in group II fipronil @24.25 mg/kg b. wt. po, in group III HSEAH @ 100 mg/kg b wt. po, and in group IV HSEAH 100 mg/kg b. wt. plus fipronil @24.25 mg/kg b. wt. po., were given for 90 days. The reduction in body weight and organ weight significantly decreased in fipronil group. However, reduction in body weight was restored in HSEAH treated group IV. Fipronil exposure caused significant (P<0.05) reduction in Hb, PCV, MCV, MCH, MCHC, TLC and DLC as compared to control groups. A significant (P<0.05) decline in total protein, albumin and globulin was observed in group II as compared to control. Fipronil plus HSEAH treated group IV showed significant (P<0.05) amelioration in the level of total proteins as compared to group II and at par with normal values in control showing ameliorative effect of HSEAH. A significant (P<0.05) increase in AST, ALT, ALP, LDH, glucose, creatinine, BUN, cholesterol, triglycerides and LDL were significant decreased in fipronil treated group II, which were restored by HSEAH towards normalcy indicating amelioration of these parameters by HSEAH. A significant (P<0.05) decline in catalase, GSH and SOD and an increase in LPO in RBCs and tissues was observed in group II which, however, returned to normalcy following simultaneous administration of HSEAH in group IV after 90 days. Administration of HSEAH alone in group III significantly elevated the catalase, GSH and SOD activity as compared to control group I. Histopathological changes were observed in liver, kidney, lungs, spleen, heart, intestine, testis, brain and thyroid fipronil treated rats which were ameliorated by treatment with HSEAH after 90 days in rats. Gene expression study revealed that expression of CYP3A1 gene was upregulated in fipronil treated rats and down regulated in group IV treated with fipronil plus seed extract as compared to animals of control group I. A significantly (P<0.05) high sperm morphological abnormalities and decrease in sperm motility, sperm viability, and sperm density was observed group II rat whereas, treatment with HSEAH restored these parameters towards normal values. A comparison of tissue residue study in between fipronil alone and combination with HSEAH, detected significantly (P<0.05) higher fipronil residues in the tissues of group II in comparison with group IV rats indicating ameliorating efficacy of HSEAH. It is concluded from this study that the extract of HSEAH exhibited the antioxidant property. Administration of fipronil @24.25 mg/kg b.wt. po, produced haemotoxic, hepatotoxic, nephrotoxic, cardiotoxic, reproductive toxic and oxidative, which were ameliorated following simultaneous administration of Amaranthus hypochondriacus seed extract @ 100 mg/kg b. wt. po, for 90 days in rats.