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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Analysis of economic traits and expression profile of different growth related genes in IGF-1 SNP genotypes of Indian colored broilers
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-06) Pandey, Nitin Kumar; Sharma, R.K.
    IGFs are involved in metabolism and body growth by regulating the synthesis and degradation of glycogen and proteins in animals. In chicken IGF-1 gene, an A570C substitution has been reported to invariably associate with growth, body composition, skeletal integrity, hormonal and metabolic factors. The present study aimed to detect polymorphism in the respective region (promoter and 5’ UTR) in CSML individuals in two generations using Hinf I PCR-RFLP. An array of economic traits viz. Juvenile body weights, Body conformation traits, Carcass traits and crude protein% in breast muscle were recorded on birds of different genotypes of IGF-1. Expression analysis of various growth related genes i.e. IGF-1, IGF-2, IGF-1 receptor, Growth hormone receptor, IGFBP-2, Ghrelin, Myostatin and TGF-β2 were also carried out using real time PCR in liver and breast muscle samples of birds of each of IGF-1 genotype at 45 days of age. Genomic DNA has been isolated from blood using phenol choloroform extraction method. A total of 100 and 96 birds were screened for estimating gene and genotypic frequencies in two successive generations. PCR amplified fragment of 813bp was digested with Hinf I. The Hinf I digestion revealed patterns viz. AA (378, 244 and 191bp), BB (622 and 191bp) and AB (622, 378, 244 and 191bp) in both the generations. The overall frequency of AA, AB and BB genotypes were 0.74, 0.23 and 0.03; in generation I these were 0.70, 0.27 and 0.03; and in generation II 0.78, 0.18 and 0.04, respectively. The frequency of allele A and B in generation I was 0.83 and 0.17 and in II generation 0.87 and 0.13, respectively, with an overall frequency of A and B alleles as 0.85 and 0.15, respectively. Significant differences among IGF-1 genotypes were observed on body weights at III, IV and V weeks of age in first generation and on body weight at V and VI weeks of age in II generation. IGF-1 AA and IGF-1 AB genotype exhibited higher body weights than IGF-1 BB genotype birds. Significant effect of IGF-1 genotypes on conformation traits was observed in both the generations. IGF-1 AA and IGF-AB genotypes had significant effect on all the carcass traits except EY%, heart, gizzard, giblet and abdominal fat in first generation and EY%, spleen and bursa in II generation. For cutup parts IGF-1 genotype had significant effect on back, thigh, drumstick and wing in first generation and on all the cut-up parts except back weight in II generation. IGF-1 AA genotype birds exhibited significantly higher CP% in breast muscle as compared to that IGF-1 AB genotype birds. Expression profile of all the genes studied showed pattern of higher expression in hepatic tissue of AB and AA genotypes as compare to BB genotype. However, except myostatin, the picture was reverse in PM muscle, where expression was higher in IGF-1 BB genotype.
  • ThesisItemOpen Access
    Analysis of economic traits and expression profile of different growth related genes in IGF-1 SNP genotypes of Indian colored broilers
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-06) Pandey, Nitin Kumar; Sharma, R.K.
    IGFs are involved in metabolism and body growth by regulating the synthesis and degradation of glycogen and proteins in animals. In chicken IGF-1 gene, an A570C substitution has been reported to invariably associate with growth, body composition, skeletal integrity, hormonal and metabolic factors. The present study aimed to detect polymorphism in the respective region (promoter and 5’ UTR) in CSML individuals in two generations using Hinf I PCR-RFLP. An array of economic traits viz. Juvenile body weights, Body conformation traits, Carcass traits and crude protein% in breast muscle were recorded on birds of different genotypes of IGF-1. Expression analysis of various growth related genes i.e. IGF-1, IGF-2, IGF-1 receptor, Growth hormone receptor, IGFBP-2, Ghrelin, Myostatin and TGF-β2 were also carried out using real time PCR in liver and breast muscle samples of birds of each of IGF-1 genotype at 45 days of age. Genomic DNA has been isolated from blood using phenol choloroform extraction method. A total of 100 and 96 birds were screened for estimating gene and genotypic frequencies in two successive generations. PCR amplified fragment of 813bp was digested with Hinf I. The Hinf I digestion revealed patterns viz. AA (378, 244 and 191bp), BB (622 and 191bp) and AB (622, 378, 244 and 191bp) in both the generations. The overall frequency of AA, AB and BB genotypes were 0.74, 0.23 and 0.03; in generation I these were 0.70, 0.27 and 0.03; and in generation II 0.78, 0.18 and 0.04, respectively. The frequency of allele A and B in generation I was 0.83 and 0.17 and in II generation 0.87 and 0.13, respectively, with an overall frequency of A and B alleles as 0.85 and 0.15, respectively. Significant differences among IGF-1 genotypes were observed on body weights at III, IV and V weeks of age in first generation and on body weight at V and VI weeks of age in II generation. IGF-1 AA and IGF-1 AB genotype exhibited higher body weights than IGF-1 BB genotype birds. Significant effect of IGF-1 genotypes on conformation traits was observed in both the generations. IGF-1 AA and IGF-AB genotypes had significant effect on all the carcass traits except EY%, heart, gizzard, giblet and abdominal fat in first generation and EY%, spleen and bursa in II generation. For cutup parts IGF-1 genotype had significant effect on back, thigh, drumstick and wing in first generation and on all the cut-up parts except back weight in II generation. IGF-1 AA genotype birds exhibited significantly higher CP% in breast muscle as compared to that IGF-1 AB genotype birds. Expression profile of all the genes studied showed pattern of higher expression in hepatic tissue of AB and AA genotypes as compare to BB genotype. However, except myostatin, the picture was reverse in PM muscle, where expression was higher in IGF-1 BB genotype.