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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Studies on the effects of tiletamine-zolazepam, propofol and sevoflurane anaesthesia in dexmedetomidine and butorphanol premedicated dogs
    (G.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145, 2022-07) Tiwari, Kanika; Jadon, Narendra Singh
    Thirty-six adult dogs used in the study were randomly divided into 6 subgroups (A-1, A-2, A-3, B-1, B-2 and B-3). All the dogs were administered with atropine sulphate at the dose rate of 0.04 mg/kg body weight subcutaneously. After five minutes, animals of group A-2 and B-2 were premedicated with dexmedetomidine whereas animals of group A-3 and B-3 were premedicated with dexmedetomidine and butorphanol at the dose rate of 5 μg/kg body weight and 0.1 mg/kg body weight intravenously. The animals of groups A-1, A-2, A-3, B-1, B-2 and B-3 induced with tiletamine-zolazepam and propofol at the dose rate of 6.66±0.06 mg/kg iv, 1.86±0.11 mg/kg iv, 1.26±0.02 mg/kg iv, 5±0.06 mg/kg iv, 2.56±0.01 mg/kg iv and 2.16±0.01 mg/kg iv respectively. Anaesthesia was maintained with sevoflurane and MAC of sevoflurane was determined by tail clamp method in all the animals. The level of anaesthesia was determined by observing various clinical (induction time, duration of anaesthesia, muscle relaxation, pedal reflex, palpebral reflex, analgesia, recovery time, sternal recumbency time, standing time, complete recovery time, required doses of induction agents and reduction in the % MAC of sevoflurane, physiological and haemodynamic (rectal temperature, heart rate, respiration rate, blood pressure, haemoglobin oxygen saturation (SpO2), electrocardiography), haematological (haemoglobin, total erythrocyte count, total leucocyte count, differential leucocyte count) and biochemical (serum glucose, total protein, serum albumin, serum urea nitrogen, serum creatinine, alanine amino transferase, aspartate amino transferase) parameters. Clinical efficacy of the anaesthetic combination was determined by performing various surgical operations.The mean induction time reduced in the animals of groups A-2, A-3, B-2 and B-3 after the administration of preanaesthetic drugs whereas longer duration of anaesthesia, recovery time, sterna recumbency time, standing time and complete recovery time were observed in the animals of group A-2, A-3, B-2 and B-3 after the administration of preanaesthetic drugs. Adequate muscle relaxation and excellent analgesia was observed in the post-induction period in all the animals of different groups. Pedal and palpebral reflexes were very weak after the induction with tiletamine-zolazepam in group A-1, A-2 and A-3 whereas it was completely lost after the induction with propofol in group B-1, B-2 and B-3. Preanaesthetic administration of dexmedetomidine and butorphanol reduced the induction dose of drugs (propofol and tiletamine-zolazepam) and MAC of sevoflurane. The rectal temperature, heart rate and respiration rate showed significant decrease at various time interval and reached to the baseline values by 24 hours post-anaesthesia. SAP, DAP and MAP was decreased non-significantly in group A-1 and B-1 whereas it showed a significant increase in group A-2, A-3, B-2 and B-3 at respective time interval. Significant decrease in haemoglobin oxygen saturation was observed after administration of induction agents. Significant reduction in PR interval was observed in animals of group A-1 whereas non-significant changes were observed in QRS and QT interval in all the groups of animals. Inverted T-wave and sinus dysrhythmia were observed after administration of dexmedetomidine whereas sinus tachycardia was observed after induction with tiletamine-zolazepam. Transient changes within physiological limits were observed in haemato-biochemical parameters which reached to baseline values by 24 hours. On the basis of above mentioned parameters, it was observed that the anaesthetic combinations used in the study have least deleterious effects on different body systems and the most efficacious anaesthetic combination was atropine sulphate-dexmedetomidine-butorphanol-tiletamine-zolazepam-sevoflurane
  • ThesisItemOpen Access
    Studies on applicability of titanium mesh cage for osteosynthesis of induced long bone segmental defects in rabbits (Oryctolagus cuniculus )
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-08) Raj Kumari; Das, Arup Kumar
    The present study was conducted in two phases. In the first phase suitable anaesthetic protocol, for the development of orthopaedic model in rabbits, was explored. It was conducted in twenty (20) clinically healthy adult New Zealand white rabbits (~1.5-2.0 years old), of either sex, weighing 1.5 to 2.0 kg and were arbitrarily allocated for anaesthetic protocol-1 and anaesthetic protocol-2 containing 10 rabbits in each protocol. The common features included in these two anaesthetic protocols that premedication and induction agents along with dose rate were kept similar and an interval of five minutes was kept between each of these injections. Afterwards anaesthesia was maintained by sevoflurane in rabbits of protocol-1 and with isoflurane in rabbits of protocol-2. On the basis of evaluation of anaesthesia related parameters - sevoflurane was found relatively a better maintenance anaesthetic agent. Thereafter sevoflorane was used during the model development and surgical repair of left tibial midshaft segmental defects (LBSDs). In the second phase of study sixteen (16) adult New Zealand white rabbits (~1.5-2.0 years old) of either sex, weighing 1.5 to 2.5 kg were selected and were divided randomly into two groups (A and B) with eight animals in each group. In these rabbits LBSDs were created and cylindrical Ti-mesh cages and full length intramedullary Ti K-wire were used as implants for osteosynthesis. In rabbits of Group-B Ti-mesh cages were filled with HA ladened Mg-gelatin-collagen-spacer. On the basis of evaluation of parameters, better wound healing was observed in rabbits of Group-B compared to Group-A rabbits. Evaluation of biochemical parameters also showed greater intensity in healing of LBSDs of Group-B. The radiological parameters showed faster rate of new bone formation, reduction in gap (1.00 cm) and remodeling of segmental defect in rabbits of Group- B. On gross observations of segmental defect at the test limb it was found that a clear visible new bone bridging the entire defect and was closely integrated with the proximal and distal host bone-cage junction was observed in Group-B rabbits. However a slightly visible gap was observed in Group -A animals of this study. Although in both the groups the implant materials remained completely stable and in correct alignment that might have adequately aided in process fracture healing. The findings of histopathological and tetracycline labeling were also corroborating the findings of clinical and radiological parameters. Conclusively a better fracture healing and bridging of critical size defect was observed in rabbits of Group B as compared to Group -A rabbits and it can be stated that spacer used in this group might have aided for it and possessing attributes of osteogenicity, biodegradibity and biocompatability. In summated form it can be stated that the outcome of surgical management of large segmental defects of the tibia using cylindrical titanium mesh cages has advantages of comparative simplicity and versatility. So it has the scope to be used for the treatment of long segmental bone defects in clinical veterinary practice. However, further clinical studies are required in order to evaluate of its efficacy.
  • ThesisItemOpen Access
    Studies on regenerative potential of bone marrow derived differentiated osteoblasts and their gene expression in fracture repair in rabbit model
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-03) Bhatt, Jyotsana; Jadon, Narendra S.
    The present study was conducted to evaluate regenerative potential of bone marrow derived differentiated osteoblast along with β-TCP scaffold in augmenting fracture healing in rabbits and to delineate expression profile of various genes involved in fracture repair as molecular markers of healing via Real time-PCR. During first phase of study isolation and propagation of bone marrow derived MSCs, its differentiation into osteoblasts and their characterization utilizing specific techniques was done followed by second phase of clinical evaluation of therapeutic potential of undifferentiated MSCs and differentiated osteoblasts in fracture repair in rabbit model. Thirty six rabbits used in this study were randomly divided into four equal groups (A, B, C and D) having 9 animals each. Animals were anaesthetized with xylazine @ 6mg/kg followed by ketamine @ 60mg/kg, intramuscularly and a 7mm segmental defect was created in mid diaphysis of left radius bone. Animals of different groups were subjected to different treatment protocols. Animals of group I kept as negative control and only external splint was applied. In animals of group II, only β- TCP implant was used to bridge the segmental defect. Animals of group III were treated with undifferentiated MSCs along with β- TCP implant and animals of group IV were treated with differentiated osteoblasts along with β- TCP implant. Quality of fracture repair was assessed by clinical parameters, radiography, estimation of biochemical parameters, gross evaluation of test bone, histopathology, histochemistry using Van Gieson’s staining, flourochrome labelling, scanning electron microscopy and molecular assessment of bone turnover markers via Real time-PCR. Radiographic findings, gross evaluation of test bone and histopathology revealed that the key criteria for evaluating bone healing like level of callus formation, bony union between cut ends and bone remodelling were significantly (P<0.05) prominent in animals of group IV treated with differentiated osteoblasts along with β-TCP implant closely followed by animals of group III treated with undifferentiated MSCs along with β-TCP implant. Biochemical parameters showed significant (P<0.05) increase in its level on day 30 postoperatively in all the groups, however these values returned to normal level at day 45 in group III and IV signifying faster pace of healing in stem cell treated groups. Tetracycline labelling and scanning electron microscopic study of regenerated tissue from different groups of animals revealed significantly (P<0.05) superior quality of healing in animals of group IV. Gene expression profiling showed upregulation of osteopontin and osteocalcin genes, associated with remodelling, in animals of group IV whereas genes like alkaline phosphatise and collagen type-1, associated with proliferative stage of fracture healing, were upregulated in animals of group I. Results of this study reveals that animals treated with osteogenically predifferentiated MSCs (osteoblasts) showed rapid and superior quality healing of critical sized segmental bone defects as compared to animals treated with undifferentiated MSCs, β-TCP implant alone and control animals.