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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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Subject: Veterinary Surgery and Radiology × Author: Khalid Mubarik × End date: 2019 × Start date: 2016 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    Isolation, propagation and characterization of bone marrow derived mesenchymal stem cells and their differentiation into cardiac myocytes in canines
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-07) Khalid Mubarik; Kandpal, Manjul
    Mesenchymal stem cells are valuable source of multipotent cells which have the ability to differentiate into various types of tissue cells and contribute to the regeneration of a variety of mesenchymal tissues including bone, cartilage, muscle, and adipose. Bone marrow-derived mesenchymal stem cell (MSC)-based therapy is an emerging approach for the treatment of both acute and chronic heart failure. The aim the current study was to focus on canine mesenchymal stem cells and to analyse their differentiation capacity into cardiomyocyte phenotype in vitro. An adult mongrel dog of 5 years of age weighing 25 kgs was used in this study. Bone marrow was aspirated from iliac crest after anaesthesia using xylazine and ketamine. Mesenchymal stem cells were harvested using density gradient centrifugation from the mononuclear cells of bone marrow. The cells were seeded in tissue culture flasks and allowed to grow in monolayer till the attainment of 80-90% of confluency. At this confluency passaging was done using trypsin-EDTA. The cells were subcultured upto four passages. These cells were characterized at their baseline for morphology, culture behavior and also by scanning electron microscopy. At passage three, the cells were exposed to medium containing 5-Azacytidine (10µl/ml) to induce differentiation into cardiac myocyte. The media was changed into media without 5-Azacytidine after 24 hours. The cells were cultured for 21 days in an incubator maintained at 5% CO2 and 37oC. The differentiation of these cells was then analysed using cellular morphology, gene expression and scanning electron microscopy. PCR was carried out for expression of genes Nkx 2.5, Cardiac troponin-T (cTnT), β-Cardiac myosin in differentiated cells. SEM was also done for analyzing the morphology of differentiated cells. Canine MSCs demonstrated morphological changes towards cardiomyocyte like phenotype during differentiation. Cells assumed a stick-like morphology and were connecting with adjoining cells forming myotubelike structures. The cells demonstrated increased expression of cardiac specific markers. On the basis of results obtained in this study it was concluded that Mesenchymal stem cells possess potential for differentiating into cardiac myocyte like cells in vitro. The exploitation of these in vitro harvested cardiac myocyte like cells may prove an effective therapy for cardiac repair under various pathological conditions.