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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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Subject: Veterinary Surgery and Radiology × Author: Joshi, Pratibha × End date: 2019 × Start date: 2016 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    Isolation, propagation and characterization of bone marrow derived mesenchymal stem cells and their differentiation into chondrocytes in dogs
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-06) Joshi, Pratibha; Jadon, Narendra Singh
    The intriguing biology of stem cells and their vast clinical potential is emerging rapidly for gene therapy. Bone marrow stem cells are contemplated as potential targets for cell and gene therapy-based approaches against a variety of different diseases. The leitmotif of this study was differentiating cBM-MSCs into chondrocytes in vitro. The aspiration of bone marrow was done aseptically from ileac crest of the mongrel dog. The procedure was done after administration of general anaesthesia. The aspirate from bone marrow was processed within half an hour of its collection. Pellet of MSCs along with mononuclear cells were isolated by density gradient centrifugation technique. Mesenchymal stem cells were isolated on the basis of plastic adherence and elongated spindle –shape. At 80-90% confluency, cells were passaged using Trypin-EDTA to amplify the cell population. Passaging of cells were done upto 4 passages and then MSCs were grown in chondrogenic differentiation media in CO2 incubator at 37 ° C and 5% CO2 for 21 days. The morphology of cells was transitioned from spindle elongated shape to round/ spherical shape. The cells were characterized at regular intervals by staining with Toluidine blue at 7th and 21st days with positive result which coloured the extracellular matrix purple. The RT-PCR for the genes SOX9 and COMP at 10th day supported with Type II collagen and Aggrecan at 22nd day revealed bands confirming the differentiation process. The sample on gelatin scaffold and on pellet when subjected to scanning electron microscopy revealed round shape cells with cell aggregates. It was concluded with the support of this study that MSCs possess potential to differentiate into chondrocytes when cultured in chondrogenic medium in vitro and techniques for identification are affluent. It had nevertheless paved the way for the establishment of regenerative and gene therapy for repair and regeneration of damaged and diseased tissue.