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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Isolation and propagation of muscle-derived mesenchymal cells and assessment of their regenerative potential in skeletal muscle injuries in mice
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-08) Godiyal, Akanksha; Kandpal, Manjul
    The present study was conducted on 24 clinically healthy Swiss albino mice (8-10 weeks old) of either sex, weighing 25-35 gm. The animals were divided into three groups viz. group A, B and C, having 8 animals in each. In all the groups, the gastrocnemius muscle was injured by surgical resection of the muscle. After creating muscle injury, PBS was injected in the mice of group A (control group) on day 1 and 5. In the mice of group B, MDSCs were injected in the injured muscle on day 1 and 5. While in the mice of group C, MDSCs were injected in the injured muscle on day 5 and 10. Muscle-derived mesenchymal cells were isolated by modified preplate method and cultured in proliferation media and incubated at 37 °C in a humidified 5% CO2 incubator. When the cells reached 70-80 % confluency, they were harvested using 0.25% trypsin-EDTA and cells were implanted at the site of injury in animals of group B and C. The regenerative potential of MDSCs for repair of the injured muscle was assessed by the evaluation of physical parameters, physiological parameters, wound condition, biochemical parameters, histopathological studies and free wire hanging test at different time intervals. Also, gene expression profiling of mVEGF was done for the evaluation of muscle regeneration via angiogenesis on day 7 and 15 after creation of muscle injury wound. On the basis of parameters observed in the present study, early and better healing of injured muscle was revealed in the mice of group B. There was early reduction in swelling, exudation, warmth and pain at the repaired site in the mice of group B. The level of enzymes CK, LDH, AST and AST was decreased significantly in the mice of group B. Also, the score of free wire hanging test was highest in the mice of group B as compared to the mice of group A and C indicating complete regeneration of the injured muscle. Histopathological observations revealed the presence of well organized, polygonal shaped regenerated myofibers having peripherally arranged nucleus in the mice of group B. Gene expression analysis showed upregulation of the gene mVEGF in group B as compared to control group. Results concluded that muscle-derived mesenchymal cells can be isolated by modified preplate method, cultured in-vitro and could be used without any scaffold for regeneration of injured skeletal muscle. Therefore, the current study confirmed that muscle-derived mesenchymal cells may be used for early, better and complete muscle regeneration in clinical cases of muscle injuries.