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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Studies on virulence, antimicrobial resistance and genetic diversity of Salmonella typhimurium isolates from North india
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2016-08) Prasanna Kumar, V.; Singh, S.P.
    Salmonellosis is one of the most frequently reported food-borne diseases world-wide commonly caused by Salmonella Typhimurium and Salmonella Enteritidis serovars. The present study was undertaken to understand the pathogenic nature, antimicrobial resistance pattern and heterogeneity of S. Typhimurium isolates (n=94) of diverse origin from northern states of India (Uttar Pradesh, Uttarakhand, Bihar, Assam and Jammu & Kashmir). All the 94 isolates were confirmed to be S. Typhimurium through various morphological, biochemical, serological and molecular methods. Salmonella Typhimurium isolates revealed varying distribution pattern of virulence viz., invA (100 %), sipA (100 %), sopE1 (61.7%), fliC (80.85%), mgtC (100 %), spvC (12.76%). stn (89.36%), sopB (94.68%) and gipA (45.74%). The presence of virulence determinants located on prophages (sopE1 and gipA) and plasmids (spvC) of S. Typhimurium isolates from North India was found to be less compared to the presence of virulence genes located on SPIs (invA, sipA, fliC, mgtC, stn and sopB). Antimicrobial susceptibility testing revealed higher resistance against nalidixic acid and sulfafurazole (28.72% each) followed by tetracycline (14.89%), ampicillin and ciprofloxacin (12.76% each), levofloxacin and norfloxacin (11.7% each). This appears to be the first study to report the emergence of S. Typhimurium isolates (animal origin) resistant to third generation fluroquinolones in Patna (Bihar) and Pantnagar (Uttarakhand). The findings also suggest the presence of MDR strains of S. Typhimurium in northern states of India except Assam. The present study also detected various antimicrobial resistance genes corresponding to the respective antibiotic classes viz., blaTEM, strA, strB, tet(A), sul1 and sul2 along with class 1 integron. The class 1 integron were identified with two type of gene cassette viz., dfrA5 and aac(3-Id)-aadA7 0.4 kb and 1.3 kb size, respectively. Genetic diversity analysis performed by ERIC-PCR revealed a discriminative index of 0.7817 while, one of the isolates (S145) from Guwahati (Assam) was found untypable. The PFGE analysis of 16 isolates revealed a discriminatory power of 0.9 and it was proved to be the more efficient tool in comparison to ERIC-PCR. The genetic similarity among the isolate from different sources and locations indicate their common origin.
  • ThesisItemOpen Access
    Molecular cloning, expression and In-silico characterization, immunogenicity, Salmonella typhimurium, polymerase chain reaction, isolates, immunity
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2012-01) Rajeev Kumar; Singh, S.P.
    The present investigation was undertaken to isolate and characterize the immunogenic Omp28 protein from S. Typhimurium. The Genus-specific PCR assay was standardized with aview to amplify specific region of 16S rDNA gene and subsequently subjected for molecular characterization for 28 different field isolates of Salmonella from different sources. Theisolates were S. Abortuseqaui, S. Anatum, S. Agona, S. Bareilly, S. Bonariensis, S. Bredeney,S. Berta, S. Bergen, S. Choleraesuis, S. Derby, S. Enteritidis, S. Gallinarum, S. Greenside, S. Heidelberg, S. Illinois, S. Infantis, S. Java, S. Kentucky, S. Newport, S. Paratyphi-B, S. Pollurum, S. Rubislaw, S. Saintpaul, S. Stanley, S. Seftenberg, S. Typhi, S. Typhimurium and S. Weltevreden along with standard Salmonella Typhimurium culture (MTCC-3214). All the samples revealed a specific banding pattern of amplified PCR products with a presence of specific 574bp band, for genus Salmonella. In order to determine Salmonella genus specific Omp28 gene, PCR condition was standardized, gene was amplified and sequenced. All these serovars were also subjected to PCR for determination of presence of immunogenic Omp28 gene in the form of specific amplified products size specifically of 341bp. The outer membrane protein was analyzed using SDS-PAGE which revealed the presence of low molecular weight of 12.32kDa main protein as outer membrane protein of Salmonella Typhimurium. The Immunogenic Omp28 gene of S. Typhimurium was successfully cloned in cloning Vector(pUC19) and confirmed by nucleotide sequencing. The construct pUC19-Omp28 was further Sub-cloned in expression vector pET32a(+). The expression construct pET32a(+)-Omp28 was then used for expression of protein through induction with IPTG and expressed fusion recombinant protein was further characterized through SDS-PAGE analysis, which shows about 29.59 kDa fusion expressed recombinant proteins, consisting of about 17.27 kDa fusion tag of pET32a(+) expression vector and about 12.32 kDa of expressed protein of Omp28 gene of S. Typhimurium which was inserted in expression vector. The sequenced Omp28 gene of S. Typhimurium, S. Enteritidis and S. Paratyphi-B was subjected to in-silico characterization and structure determination (primary, secondary and 3D structure of amino acid sequence derived from sequenced gene). Further phylogenetic tree analysis, B-cell epitope, MHC I, II binding prediction with Allele HLA (Human) and homology modeling was also carried out with predicted amino acid of proteins. Analysis of Pfam showed that the protein belong to Pfam A family, hdeB super family [PRK11566]. The immunogenicity of protein was computed through antigenic plot and found antigenicity index of Omp28 protein were about 2.2 (0.1-2.2) for S. Typhimurium and S. Enteritidis and for S. Paratyphi-B about 2.1(0.1-2.1). More than 1.2 antigencity index suggests that all isolates possessing Omp28 gene protein can trigger the immune system and produce antibody in vivo. i.e. this expressed protein from Omp28 gene could be immunogenic in nature.