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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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2006 - 20072
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Subject: Veterinary Microbiology × End date: 2008 × Start date: 2005 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    Isolation and characterization of fowlpox virus of poultry
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2007-07) Madanpal; Rao, V.D.P.
    Fowl pox is a contagious and slow spreading viral disease. It affects the birds of all age, sex and breeds. The disease is manifested in three different clinical forms–cutaneous, diphtheritic and oculo nasal forms. The mucosal lesions involving the mouth, esophagus and trachea can be confused with other respiratory diseases like infectious laryngotrachaetis, coryza etc. Keeping in view of the impact of disease on economics of poultry industry, the present study was undertaken to isolate the virus from scab lesions of birds of a poultry farm near Barielly following isolation of virus on chorioallantoic membrane (CAM), of developing chicken embryos, further characterization was carried by studying cytopathogenicity in cell culture and sero diagnostic tests. It was observed that virus was successfully adapted to CAM, CEF cells as well as in BHK 21 cell line. Characteristic pock lesion in CAM and CPE in unstained and stained preparations confirmed the presence of virus. In MGG staining, the cytopathic changes characterized by rounding of cells 24 hrs PI and the cytoplasmic vacuolation and syncytia formation by 48 hrs PI. In few cells, the nucleus occupied eccentric position and degenerative changes in the nucleus characterized by fragmentation of nuclear membrane in the infected CEF cells while the cytopathic changes in infected BHK 21cells were characterized by rounding of cells 36 hrs PI and the cytoplasmic vacuolation and syncytia formation by 48 hrs PI. The infectivity titre was calculated to be log104.25/ml (EID50/ml) on CAM and log10 9.79/ml (TCID50/ml) in CEF cell culture. The agar gel precipitation test (AGPT) revealed the precipitation band, which confirms the presence of antigen and antibody. Counter immunoelectrophorasis (CIE) showed a precipitation line within one hr of electrophoretic run. The indirect fluorescent antibody technique (IFAT) was used to demonstrate the virus in infected cell culture and cell line. The infected chicken embryo fibroblast cells revealed small particulate fluorescence in the cytoplasm of the cells. These tests confirmed that the virus isolate as fowlpoxvirus SDS-PAGE analysis of cell culture supernatant infected with FPV isolate revealed 11 polypeptides with molecular weights ranging from 120k Da to 15 kDa.
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    ThesisItemOpen Access
    Studies on vaccine strain of Fowlpoxvirus with special reference to its adaptation in primary cell cultures and its characterization
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2006-08) Tripathi, Ashok Kumar; Rajesh Chandra
    Fowlpox is an economically important and widespread disease of poultry that causes heavy morbidity and mortality in the affected birds resulting in heavy losses in the form of low egg and meat production. It affects the birds of all age, sex and breeds. The disease is caused by Avipoxvirus of the family Poxviridae. The disease is manifested in three different clinical forms–cutaneous, diphtheritic and oculo nasal forms. The mucosal lesions involving the mouth, esophagus and trachea can be confused with other respiratory diseases like infectious laryngotrachaetis, coryza etc. Keeping in view the economic impact of disease on poultry industry, the present study was undertaken to adapt the vaccine strain of FPV in chorioallantoic membrane, chicken embryo fibroblast cell culture as well as in chicken kidney cell culture and evaluate the various serological tests for rapid diagnosis of FPV infection. Fowlpoxvirus was successfully adapted to CAM, CEF and CK cells. Characteristic pock lesions on CAM and CPE in unstained and stained preparations confirmed the presence of virus. In MGG staining, the cytopathic changes were characterized by rounding of cells 12 hrs PI and the cytoplasmic vacuolation and syncytia formation by 18 hrs PI. In few cells, the nucleus occupied eccentric position and degenerative changes in the nucleus were characterized by fragmentation of nuclear membrane in the infected CEF cells, while the cytopathic changes in infected CK cells were characterized by rounding of cells 36 hrs PI and the cytoplasmic vacuolation and syncytia formation by 48 hrs PI. The infectivity titre was calculated to be log107.14 EID50/ml on CAM and log10 9.25TCID50/ml in CEF cell culture. The agar gel immunodiffusion test revealed the precipitation band, which confirms the presence of antigen and antibody. The serum neutralization test using the beta (constant virus serum dilution) procedure, revealed an antibody titre of 1:160. CIE showed a precipitation line within one hr of electrophoretic run. The indirect fluorescent antibody technique (IFAT) demonstrated the presence of virus in infected cell culture. The infected embryonic chicken kidney cells as well as the infected chicken embryo fibroblast cells revealed small particulate fluorescence in the cytoplasm of the infected cells.