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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    X-sperm enrichment of bovine semen by percoll density gradient method and its effect on fertility of semen, sex ratio and conception rate
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-08) Bhat, Mohd Yaqoob; Sharma, Mridula
    The present research work was conducted to investigate the effect of percoll density gradient method of X-sperm semen enrichment on fertility of semen, conception rate and sex ratio. Semen samples collected by artificial vagina method from two cross-bred bovine bulls with mass motility of ˃ +3 and progressive motility ˃ 70% were considered for the study. Fresh semen was centrifuged twice in percoll density gradient medium composed of 90%, 70% and 50% percoll gradients with dense layer at bottom and lighter layer at top at 500xg for 15 minutes at 22-24ºC. X-spermatozoa enriched semen pellet after 2nd centrifugation was diluted in EYC dilutor and used for AI within 48 hours of semen collection. The effect of percoll density gradient on semen quality (fertility) was revealed by evaluating various seminal parameters like volume, pH, motility, viability, concentration, metabolic activity (MBRT), membrane integrity (HOST) and acrosomal integrity at three stages; before centrifugation (stage I), after 1st centrifugation (stage II) and after 2nd centrifugation (stage III) respectively. The pregnancy rate was detected at 45-60 days post AI. The sex of fetus was detected at 60-80 days post AI by locating the position of genital tubercle with the help of ultrasonography. There was non-significant (P ˃ 0.05) change observed in pH and mass motility of semen at all three stages. The volume, progressive motility, concentration and HOST reactive sperms significantly decreased at stage ІІ and stage І ІІ compared to stage І with no significant difference between stage ІІ and stage ІІ І . The percent live sperms were significantly (P<0.05) higher at stage І compared to stage ІІ and stage І ІІ . There was also significant decrease (P<0.05) in percent live sperms at stage ІІІ compared to stage ІІ . The time taken to reduce methylene blue at stage ІІ І and stage ІІ was significantly (P<0.05) higher compared to stage І . There was non-significant effect on percent of spermatozoa with intact acrosomal membrane and fully damaged acrosomal membrane at all three stages however, partially damaged acrosomal membrane sperms were significantly (P<0.05) decreased at Stage ІІ and Stage ІІІ compared to Stage І with non-significant difference found between stage І І and stage І І І . The conception rate significantly (P<0.05) decreased with X-enriched semen compared to non-sexed liquid semen. However, it was significantly (P<0.05) higher compared to the conception rate with sexed semen from flow cytometry. The female sex ratio of calves significantly (P<0.05) increased to 66.66% with X- sperm enriched semen compared to the male percentage of 33.33% with sexed semen and female percentage of 46.15% with non-sexed semen. In conclusion, centrifugation of fresh bovine semen twice in percoll density gradient medium did not affect the sperm quality much but, resulted in decrease in conception rate as compared to nonsexed semen and deviation of 66.66% sex ratio towards female calves.