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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Expression and evaluation of immune-potential of recombinant penton base and fibre proteins of Inclusion body Hepatitis-Hydropericardium Syndrome (IBH-HPS) virus of domestic fowl
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-07) Trivedi, Raj Narayan; Rajesh Kumar
    Present study deals with epidemiology and phylogenetic analysis, expression and immune potential of recombinant penton base and fibre proteins of IBH-HPS virus isolates recovered from natural outbreaks. Epidemiological analysis revealed that mortality ranged from 0.5% to 20% and disease outbreak was mainly observed in 21 to 42 days old broiler chickens. Twenty samples, found positive for IBH-HPS virus antigens serologically by AGID, were subjected to virus isolation in CEL culture. Followed by DNA was extracted from infected CEL cells (4th passage). Amplification of L1 loop region (~900bp) of hexon gene was done followed by RFLP analysis with six restriction enzymes which revealed two RFLP pattern. L1 loop region of hexon gene amplicon was cloned and sequenced to determine serotype of IBH-HPS isolates, which indicated that nineteen isolates are FAdV- 11/2 while one is FAdV-8b. L1 loop sequence based phylogenetic analysis revealed that our sequences clustered into one major cluster having similarity with the Fowl adenovirus class D, E and C along with strains FAdV-2, 4, 10, 11, 8b. Penton base and fibre genes from selected isolate (Pantnagar/HA-14/R-21) were PCR amplified and amplicons of about 1713bp and 1668bp respectively were obtained. Cloning and sequencing of the penton base and fibre gene was done and checked for the presence of complete ORF. Both the genes were expressed in E.coli and expressed proteins were found to be immunoreactive in western blotting with known serum. Immune – potential of recombinant proteins was evaluated by inoculating with FCA in SPF chicks. Maximum protection (80%) was observed with fibre protein and antibody titre was maximum for fibre protein at every point of time. Virus shedding was observed in feces for shortest period i.e. upto 8th day post challenge in chicks receiving fibre protein.