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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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2004 - 20096
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Subject: Biochemistry × End date: 2009 × Start date: 2004 × Thesis Type: Ph.D ×
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Now showing 1 - 6 of 6
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    ThesisItemOpen Access
    Biochemical and molecular tools for screening ashwagandha genotypes combining phenotypic and chemotypic attributes for elite germplasm
    (G.B. Pant University of Agriculture and Technology, Pantnagar (Uttarakhand), 2006) Punetha, Himanshu; Gaur, A.K.
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    ThesisItemOpen Access
    Genetic Delineation Of Molecular Basis Of Resistance Against Isoproturon In Phalaris Minor Retz.
    (Govind Ballabh Pant University of Agriculture and Technology;Pantnagar, 2005) Kachare, Dilipkumar; Mishra, D. P
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    ThesisItemOpen Access
    Characterization And Cloning Of Alpha-Amylase Inhibitor Gene From Indian Wheat And Millet
    (Govind Ballabh Pant University of Agriculture and Technology;Pantnagar, 2004) Singh, Annika; Mishra, D.P.
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    ThesisItemOpen Access
    Biochemical, physiological and molecular aspects of drought and cold tolerance in finger millet (Eleusine coracana L.) germplasm
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2008-08) Rai, Shubha; Agarwal, Sanjeev
    Finger millet (Eleusine coracana L.) is considered a drought-tolerant crop species; however, within this species, there are considerable genotypic differences in tolerance to this environmental stress. In the present work, the effect of mannitol induced drought stress on germination, plant growth, proline and hydrogen peroxide content in ten finger millet genotypes was analyzed. These genotypes were further explored for their biochemical adaptation mechanism of drought and cold tolerance in the glasshouse condition. Different biochemical parameters viz. MDA content, hydrogen peroxide content, chlorophyll content APX activity, ascorbate content and proline were determined under drought and cold stress. PRM-6107, VL-283 and VL-328 registered minimum reduction in per cent germination, shoot length and root length; however, genotype PES-400, VR-708, VL-149 and VL-146 registered maximum reduction in per cent germination, shoot length and root length, under mannitol induced drought stress. Accordingly they were classified as relatively tolerant and susceptible group, respectively. Relatively tolerant genotypes registered lower hydrogen peroxide content and higher proline content compared to susceptible ones under mannitol induced drought stress. These genotypes also recorded lower MDA and hydrogen peroxide content and higher APX activity and proline content compared to susceptible one under cold and drought stress in the glasshouse condition. Biochemical responses of all the genotypes in terms of MDA, H2O2, ascorbate, proline content and APX activity were more pronounced in drought as compared to cold stress. In addition to this total leaf protein profile of all the ten genotypes under drought stress was studied. In tolerant genotypes A4 protein band with molecular weight of less than ~66 KDa was more pronounced under drought stress. During this study, efforts have been made to explore these genotypes for ascorbate peroxidase and cold shock domain protein gene(s).The APX and CSDP gene(s) are successfully amplified. Nucleotide sequence data have been submitted to GeneBank database (Accession nos. EU795006 and EU795007). These nucleotide sequences could be used as probe to fishout the corresponding full length gene in E. coracana plant.
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    ThesisItemOpen Access
    Engineering antioxidant defense mechanism to combat salinity stress in Brassica juncea
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-06) Saxena, Saurabh Chandra; Arora, Sandeep
    Brassica juncea is a widely distributed and economically important oil seed crop, grown throughout the Indian sub-continent. The production and yield statistics of the crop has recorded wide fluctuations because of several environmental factors. Salinity stress is one of the major constraints limiting the overall yield potential of Brassica. An underlying mechanism for reduction in crop yield under salinity stress is the excessive production of reactive oxygen species (ROS) that can potentially damage lipids, nucleic acids and proteins, leading to disruption of essential physiological & biochemical processes. Plants possess a complex antioxidative defense system, comprising of enzymatic and non enzymatic molecules for scavenging these ROS. Ascorbate peroxidase (APX,E.C. 1.11.1.11), an important and widely distributed antioxidant enzyme is a part of Ascorbate –Glutathione pathway in the cell. It plays a crucial role in the detoxification of hydrogen peroxide in plants. The efficient scavenging of ROS is needed for increased tolerance to salinity stress. In the present studies, experiments were conducted to determine whether over-expression of APX could provide protection against salinity stress. Cytosolic ascorbate peroxidase (apx1) gene, isolated from Arabidopsis thaliana, was chosen as the candidate gene for strengthening the antioxidative defense system of Brassica juncea. An efficient and reproducible protocol for Agrobacterium mediated transformation of Brassica juncea (var. pusa jaikisan) was developed using hypocotyl explants. Transgenic plants over-expressing apx1 gene were developed and the successful integration of apx1 gene insert was confirmed through PCR using nptII and apx1 cDNA specific primers. The growth & physiological status of the transgenic plants was assessed using parameters like photosynthetic efficiency, stomatal conductance, chlorophyll fluorescence and SPAD values. The results indicate that the physiological and metabolic status of the transgenic plants was comparable with that of the wild type plants. Leaf disc assay was performed to evaluate the salinity stress tolerance potential of transgenic plants, using standardized biochemical parameters. Under 200 mM NaCl stress, the transgenic plants decisively performed better than the wild type plants; as revealed through greater Proline accumulation, increased Chlorophyll stability index, lower chlorophyll a/b ratio, higher activities of Ascorbate peroxidase, Guaiacol peroxidase & Total peroxidase along with lower H2O2 levels and lesser membrane damage as measured through MDA contents. From the present studies it can be concluded that cytosolic ascorbate peroxidase (apx1) can be used to strengthen the anti-oxidative defense system in plants and the transgenic Brassica juncea plants developed during the study have improved tolerance against salinity stress.
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    ThesisItemOpen Access
    Molecular typing of Salmonella isolated from Gangetic water
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-01) Balwant; Mishra, D.P.
    The present study was conducted at the Animal Biotechnology Center, Pantnagar to check the level of Salmonella contamination in the river Ganga. Total 500 samples were collected from 10 stations (50 samples from each station) viz; Gangotri, Uttarkashi, Rishikesh, Haridwar, Hastinapur, Garhmukteshwer, Narora, Kanpur Allahabad and Varanasi. These samples were subjected to Salmonella specific ‘his’ gene PCR. Samples were then Biochemically screened and serotyped at National Salmonella Research Centre IVRI, Izatnagar U.P. These isolates were serotyped as S. Abuja, S. Lagos. S. Pontypridd, S. Chinkual, S. Zwickau, S. Goldenberg and S. Oritamerin. DNA from these samples were extracted. ERIC-PCR, REP-PCR and OMPs profiling were conducted for serovar identification and strain differentiation of Salmonella rare serovars. For molecular typing ERIC- PCR, REP- PCR and OMPs analysis were conducted and it was found that REP- PCR was more efficient in strain differentiation. Therefore the data of ERIC PCR and REP PCR and OMPs profiling were used in Combined Molecular Typing and it was found that combined molecular typing was best typing method. Discriminating ability of ERIC PCR, REP PCR, OMPs profiling and Combined Molecular Typing methods were calculated by Simpson’s Index of Diversity. ERIC PCR showed D value of 0.92 and REP PCR gave 0.99 Value and of OMPs profiling it was found to be 0.94, while it was found 1 in Combined Molecular Typing. A Dendrogram was constructed by using Tree Con software by feeding data of Combined Molecular Typing then similarity and dissimilarity of isolates was checked. Twenty rare isolates showed maximum similarity. Therefore it can be concluded that combined molecular typing by using ERIC-PCR, REP-PCR and OMPs profiling gave better identification of Salmonella rare isolates.