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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Screening of tea cultivars grown in Kumaon region for their total phenolics, pigments and individual catechin contents
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2005-08) Pandey, Sudhanshu; Mishra, D.P.
    Total chlorophylls, chlorophyll a , chlorophyll b, total carotenoids and total phenolics contents and HPLC profiling of various catechins and quantitative estimation of (-)EGCG,(-)EC and (-)ECG were carried out in fresh tea leaf leaves (dried immediately after sampling), of 6 varieties grown in Kumaon region and a black orthodox tea sample processed locally. The main objective of the study was to screen these varieties for their catechin contents and other flavanoid constituents. Dried leaf powder was used to estimate chlorophylls, carotenoids and total phenols. For catechins, dried leaf powder was first extracted with aqueous methanol. This extract was subjected to separation of catechins on HPLC and identification and quantitative estimation were done using standard catechins. Contents of various parameters were variable among varieties and there was no set pattern. Kangra Jat had highest content of chlorophyll a, and total carotenoids while chlorophyll b, total chlorophyll and total phenols were highest in UPAS1-9. T-78 exhibited lowest content of all these parameters. Black orthodox tea had pretty high content of chlorophylls and carotenoids but its phenolics content was lowest, perhaps due to oxidation of phenolics during processing. The HPLC profiles gave eight peaks, out of which first two peak, were probably of some non-catechin compounds, hence ignored. Out of remaining 6 peaks, 3 were of (-)EGCG,(-)EC and (-)ECG. Other three were named as U1, U2 and U3.Out of them U1 was supported to be (-)EGC. By comparison of peak areas,retention times etc., quantitative estimation of (-)EGCG, (-)EC and (-)EC was performed, which revealed these three catechins comprised nearly 0.11-0.14% of dried solids (110-140 mg g-1) and (-)EGCG was nearly (69-82 mg g-1),half of all catechins estimated. On the basis of increasing catechin contents, order of varieties was: Black Orthodox tea(commercial)
  • ThesisItemOpen Access
    Biochemical evaluation of genotypes of Jatropha curcas L., and optimization of micropropagation schedule
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2005-07) Munagapati, Sandhya; Gaur, A.K.
    Five genotypes of Jatropha curcas viz. shu03001, shu03002, shu03004, shu04001 and shu04004 were selected for their further identification and biochemical characterization. Identification of genotypes had been done on the basis of banding pattern on SDS-PAGE of total seed storage protein and its fractions viz. globulins and albumins. Based on the Jaccard‟s similarity coefficient obtained from the banding patterns of SDS-PAGE, the five genotypes are grouped into two categories. Group1 consisting of shu03001, shu03004 and shu04004 and Group2 consisting of shu03002 and shu04001. These results were based in accordance with the percentage protein content of the respective genotypes taken for study and the oil contents of these genotypes were almost comparable. However, the lipid/protein ratio was highest in shu04001. In view of optimizing micropropagation schedule, the experiments were also conducted on three genotypes of J.curcas viz. shu03001, shu03002 and shu03004 to study the effect of various parameters viz. genotypes, growth regulators and explants and their interactions on callus induction frequency. Two explants viz., leaf and petiole from all the three genotypes were placed on different combinations of growth regulators. Calluses were induced from the two explants on all types of callus induction media containing four levels of 2,4-D (1, 2, 2.5and 3mg/l) with BAP (0.1,0.2,0.25 and 0.3mg/l) and two levels of NAA (1 and 2 mg/l) with two levels of BAP (0.1 and 0.2 mg/l). Maximum callus induction frequency of 92.8, 93.1; 97.4, 98.7 and 88.8, 96.1% was observed for leaf and petiole explants of shu03001, shu03002 and shu03004 genotypes respectively. In a generalized way, it was observed that shu03004 genotype showed highest callus induction frequency followed by shu03002 and shu03001. Media with NAA (1mg/l) and BAP (0.1mg/l) showed highest callus induction frequency with both leaf and petiole explants in all the three genotypes. Amongst explants, petiole showed higher callus induction frequency followed by leaf explant on all types of callus induction media.
  • ThesisItemOpen Access
    Biochemical and molecular tools for screening ashwagandha genotypes combining phenotypic and chemotypic attributes for elite germplasm
    (G.B. Pant University of Agriculture and Technology, Pantnagar (Uttarakhand), 2006) Punetha, Himanshu; Gaur, A.K.
  • ThesisItemOpen Access
    Genetic Delineation Of Molecular Basis Of Resistance Against Isoproturon In Phalaris Minor Retz.
    (Govind Ballabh Pant University of Agriculture and Technology;Pantnagar, 2005) Kachare, Dilipkumar; Mishra, D. P
  • ThesisItemOpen Access
    Biochemical, physiological and molecular aspects of drought and cold tolerance in finger millet (Eleusine coracana L.) germplasm
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2008-08) Rai, Shubha; Agarwal, Sanjeev
    Finger millet (Eleusine coracana L.) is considered a drought-tolerant crop species; however, within this species, there are considerable genotypic differences in tolerance to this environmental stress. In the present work, the effect of mannitol induced drought stress on germination, plant growth, proline and hydrogen peroxide content in ten finger millet genotypes was analyzed. These genotypes were further explored for their biochemical adaptation mechanism of drought and cold tolerance in the glasshouse condition. Different biochemical parameters viz. MDA content, hydrogen peroxide content, chlorophyll content APX activity, ascorbate content and proline were determined under drought and cold stress. PRM-6107, VL-283 and VL-328 registered minimum reduction in per cent germination, shoot length and root length; however, genotype PES-400, VR-708, VL-149 and VL-146 registered maximum reduction in per cent germination, shoot length and root length, under mannitol induced drought stress. Accordingly they were classified as relatively tolerant and susceptible group, respectively. Relatively tolerant genotypes registered lower hydrogen peroxide content and higher proline content compared to susceptible ones under mannitol induced drought stress. These genotypes also recorded lower MDA and hydrogen peroxide content and higher APX activity and proline content compared to susceptible one under cold and drought stress in the glasshouse condition. Biochemical responses of all the genotypes in terms of MDA, H2O2, ascorbate, proline content and APX activity were more pronounced in drought as compared to cold stress. In addition to this total leaf protein profile of all the ten genotypes under drought stress was studied. In tolerant genotypes A4 protein band with molecular weight of less than ~66 KDa was more pronounced under drought stress. During this study, efforts have been made to explore these genotypes for ascorbate peroxidase and cold shock domain protein gene(s).The APX and CSDP gene(s) are successfully amplified. Nucleotide sequence data have been submitted to GeneBank database (Accession nos. EU795006 and EU795007). These nucleotide sequences could be used as probe to fishout the corresponding full length gene in E. coracana plant.