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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Immunological and molecular characterization of Marek’s disease virus isolated from field outbreaks
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2007-07) Chamling, Prerna; Umapathi, V.
  • ThesisItemOpen Access
    Isolation of species specific proteins of Salmonella and its characterization
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2006-08) Rai, Kartikey Kumar; Singh, S.P.
    Present study was conducted to find genus specific proteins of Salmonella and to study cytotoxic effects of cytotoxic preparations of Salmonella on VERO, MDBK and BHK 21 cells. It was also aimed to standardize polyclonal ELISA based test for detection of Salmonella in foods of animal origin. Cell lysates of Salmonella serovars (12), E.coli (1), Shigella flexneri and Klebsiella spp.(1), were prepared by ultrasonication. The protein concentration of cell lysate obtained from Salmonella serovars were found to range between 1.4 to 4.6 mg/ml. On the basis of consistent cytotoxic activity indicated by Salmonella Typhimurium cell lysates, PDP and CFCS of S.Typhimurium were prepared for comparative study. Cell lysates of different Salmonella serovars were tested for cytotoxicity on MDBK, BHK 21 and VERO cells. Partial purification of Salmonella protein preparations was achieved using Sephacryl S-200 HR and Sephadex G-100, which revealed four and two distinct peaks, respectively. On gel filtration through Sephacryl S-200 HR, the cell lysate of Klebsiella spp, E.coli and Shigella flexneri yielded 2,3 and 4 distinct peaks respectively. PDP and PDCL revealed two separate peaks. Pooled and concentrated peak contents were tested for cytotoxic activity on BHK 21, VERO and MDBK cells. The first and second peak content showed mild cytotoxicity, where as fourth peak content showed moderate cytotoxicity. On SDS-PAGE Cell lysate revealed 31 polypeptides. Number of polypeptides kept on decreasing as the purification steps advanced. PDCL and PDP revealed 24 and 10 polypeptide, respectively with different mol wt ranging from 126.9 to 11.2 kDa. The first, second and fourth peak contents obtained after eluting the cell lysate and Second peak of PDP of S. Typhimurium through Sephacryl S200 HR showed 14, 7, 3 and 2 bands, respectively. Immunological characterization of cytotoxic preparations was done by performing ELISA using antiserum raised against cell lysate of S. Typhimurium. On the basis of the results obtained in the present investigation it seems possible to isolate species specific cytotoxic protein which can later be used as important diagnostic tool for detection of salmonella in foods of animal origin. The difference in the chromatographic profile of Salmonella, E. coli and Klebsiella spp clearly indicate that cytotoxic fourth peak content may be specific to salmonellae except of Shigella flexneri. The cytotoxic protein of these two organism need to be studied immunologically. Further it appears from the present study that in-vitro assay model like BHK 21 and MDBK cells are quite promising and it may be used for future studies.
  • ThesisItemOpen Access
    Antigenic characterization of major cytotoxic proteins of Salmonella Weltevreden
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2005-08) Sonwane, Arvind Asaram; Ambwani, Tanuj
    In spite of years of research, Salmonella has remained to be one of the most important pathogens of humans as well as animals. Salmonellosis is the major foodborne illness world over. The menace of Salmonella can only be contained by ensuring its in-time detection in suspected food/feed. For this purpose, a rapid, sensitive and specific test based upon genus specific bacterial toxin-protein antigen may be of great value. Prior to exploring the use of these toxins as diagnostic antigens they must be studied in some detail for their antigenic characters. Meager information is available on such aspect. Considering this, the present investigation was undertaken to study the toxic/cytotoxic preparations obtained from cefotaxime extract (CE) of Salmonella Weltevreden with the help of SDS-PAGE, western blotting and circular dichroism (CD) spectroscopy. From CE, the toxic fraction viz. - 40-70% DCP (desalted and concentrated protein) was obtained by differential salt fractionation with the help of ammonium sulfate precipitation and then eventually desalting and concentrating with spin columns. Also, the sub-fractions of 40-70% DCP viz. - 40-50% DCP, 50-60% DCP and 60-70% DCP were prepared. The cytotoxic fraction from among the 40-70% DCP was separated as 2nd major peak (cytotoxic peak/CP) after elution on Sephadex G-100 column. Studies for cytotoxicity of these proteins were carried out on chicken embryo fibroblast (CEF) cell culture. The cytotoxicity shown by 2nd peak contents (CP) was characteristic. The SDS-PAGE analyses of 40-70% DCP; 40-50%, 50-60% & 60-70% DCPs and CP revealed 25, 9, 15, 22 and 7 polypeptides respectively of different molecular weights ranging from 106.3 kDa to 16.7 kDa. The toxic/cytotoxic preparations used in this experiment were highly immunogenic. On western blot analysis, 40-70% DCP revealed 14 immunogenic polypeptides in the range of 106.3 kDa to 35.1 kDa while, CP revealed 6 immunogenic bands in the range of 98.8 kDa to 52.0 kDa. This indicates that not all the proteins from these immunogenic toxic/cytotoxic preparations were immunogenic. Also, their individual immunogenicities were variable. The polypeptide, identified as SP-2 (Salmonella Polypeptide-2) was the most immunogenic one. The polypeptides in the descending order of their individual immunogenicities were SP-2, SP-9, SP-4, SP-3, SP-15, SP-1, SP-5, SP-6, SP-8, SP-16, SP-17, SP-18, SP-19 and SP-20. Secondary structure studies of the major immunogenic proteins those were observed with help of western blotting of CP were tried using CD spectroscopy. No conclusive results were obtained, which may be attributed to the probable partial denaturation of proteins during sample processing and/or the use of a CD spectra analysis database that is incompatible for the proteins of the present study. On the basis of above study it is concluded that, spin column method is superior over conventional dialysis tubing method; the CEF cell culture can be used for the cyotoxicity studies of Salmonella; the cytopathic effects induced by 2nd peak contents may be considered as „characteristic‟ for such studies in CEF cell culture system; there is a need to study the major immunogenic polypeptides obtained in this study for their immunological and molecular characters by purifying them to their homogeneity and with a more rational approach, Viz.- adopting enhanced protein purification processes and/or using proper CD data analysis database, CD spectroscopic studies should be performed for these proteins to analyze their secondary structures.
  • ThesisItemOpen Access
    Molecular characterization of field and vaccine strains of infectious bursal disease virus by reverse transcription-polymerase chain reaction and restriction fragment length polymorphism
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2005-07) Khan, Deena; Deo, Indra
    Bursal homogenates of three infectious bursal disease virus (IBDV) isolates, one from Uttaranchal (UA 5/4) and two from Tamil Nadu (TN 7/4 –A and TN 7/4 –B) were obtained and inoculated in experimental birds. Confirmation of IBDV was done by agar gel precipitation test (AGPT). All the isolates were successfully adapted to chicken embryo fibroblast cells and cytopathic effects (CPE) were observed 48-72 hours post inoculation. Amplification of 643 bp of hypervariable region of VP2 gene of IBDV was done with VP2 specific primers, using RNA isolated from bursal samples of experimentally infected birds and vaccine strain adapted to CEF cells, by reverse transcription polymerase chain reaction (RT-PCR). The restriction fragment length polymorphism (RFLP) was used to compare the amplified region of the VP2 gene among the viruses used in the study. Five restriction enzymes Dra1, Mbo1, Sac1, Ssp1 and Taq1 have been used to determine the molecular group of isolates and vaccine strain. The vaccine strain had pattern similar to that of classical and attenuated strain, which was confirmed by the presence of Sac1 site. The presence of Ssp1 site in UA5/4 in the 643 bp RT-PCR fragment was used to characterize its very virulent (vv) phenotype. TN7/4-A and TN7/4-B also had RE pattern similar to that observed in very virulent type.